Anti-Cullin 1/CUL-1抗体[AS97.1] (ab11047)
Key features and details
- Mouse monoclonal [AS97.1] to Cullin 1/CUL-1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG2a
概述
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产品名称
Anti-Cullin 1/CUL-1抗体[AS97.1]
参阅全部 Cullin 1/CUL-1 一抗 -
描述
小鼠单克隆抗体[AS97.1] to Cullin 1/CUL-1 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant full length protein corresponding to Human Cullin 1/CUL-1.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
AS97.1 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab11047于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use at an assay dependent concentration.
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说明 |
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WB
Use at an assay dependent concentration. |
靶标
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功能
Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(SKP2) directs ubiquination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110. -
组织特异性
Expressed in lung fibroblasts. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the cullin family. -
翻译后修饰
Neddylated; which enhances the ubiquitination activity of SCF. Deneddylated via its interaction with the COP9 signalosome (CSN) complex. Deneddylated by Epstein-Barr virus BPLF1 leading to a S-phase-like environment that is required for efficient replication of the viral genome. - Information by UniProt
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数据库链接
- Entrez Gene: 8454 Human
- Entrez Gene: 26965 Mouse
- Omim: 603134 Human
- SwissProt: Q13616 Human
- SwissProt: Q9WTX6 Mouse
- Unigene: 146806 Human
- Unigene: 87611 Mouse
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别名
- CUL 1 antibody
- CUL-1 antibody
- CUL1 antibody
see all
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab11047 被引用在 2 文献中.
- Youssef Y et al. Transducin ß-like protein 1 controls multiple oncogenic networks in diffuse large B-cell lymphoma. Haematologica Online ahead of print:N/A (2020). PubMed: 33054136
- Ang K et al. Mediator acts upstream of the transcriptional activator Gal4. PLoS Biol 10:e1001290 (2012). WB ; Human . PubMed: 22479149