重组Anti-CUL-4A + CUL-4B抗体[EPR3200] (ab76470)
![Western blot - Anti-CUL-4A + CUL-4B antibody [EPR3200] (ab76470)](https://www.abcam.cn/ps/products/76/ab76470/Images/ab76470-91903-anti-cullin-4a-b-antibody-epr3200-western-blot.jpg "Western blot - Anti-CUL-4A + CUL-4B antibody [EPR3200] (ab76470)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3200] to CUL-4A + CUL-4B
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-CUL-4A + CUL-4B抗体[EPR3200]
参阅全部 CUL-4A + CUL-4B 一抗 -
描述
兔单克隆抗体[EPR3200] to CUL-4A + CUL-4B -
宿主
Rabbit -
特异性
Due to the close homology between Cullin 4A and 4B this antibody is likely to react with both forms. -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: ICC/IF or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human CUL-4A + CUL-4B aa 850-950. The exact sequence is proprietary.
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阳性对照
- Fetal uterus, HeLa, A375 and fetal testis lysates and human brain tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3200 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76470于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 88, 102 kDa (predicted molecular weight: 102 kDa).
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary. |
| 说明 |
|---|
|
WB
1/2000 - 1/10000. Detects a band of approximately 88, 102 kDa (predicted molecular weight: 102 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary. |
应用说明
Is unsuitable for ICC/IF or IP.
靶标
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相关性
Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. -
数据库链接
- Entrez Gene: 8450 Human
- Entrez Gene: 8451 Human
- SwissProt: Q13619 Human
- SwissProt: Q13620 Human
图片
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Western blot - Anti-CUL-4A + CUL-4B antibody [EPR3200] (ab76470)All lanes : Anti-CUL-4A + CUL-4B antibody [EPR3200] (ab76470) at 1/5000 dilution
Lane 1 : Fetal uterus lysate
Lane 2 : HeLa lysate
Lane 3 : A375 lysate
Lane 4 : Fetal testis lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 102 kDa
Observed band size: 102,88 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CUL-4A + CUL-4B antibody [EPR3200] (ab76470)](https://www.abcam.cn/ps/products/76/ab76470/Images/ab76470-91902-anti-cullin-4a-b-antibody-epr3200-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CUL-4A + CUL-4B antibody [EPR3200] (ab76470)ab76470 at 1/100 dilution staining Cullin 4B in human brain tissue, using a HRP/AP polymerized secondary antibody.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-CUL-4A + CUL-4B antibody [EPR3200] (ab76470)](https://www.abcam.cn/ps/products/76/ab76470/Images/ab76470-3-benefits-of-recombinant-antibodies.png)
Anti-CUL-4A + CUL-4B antibody [EPR3200] (ab76470)
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab76470 被引用在 7 文献中.
- Song R et al. Knockdown of circMFN2 inhibits cell progression and glycolysis by miR-198/CUL4B pathway in ovarian cancer. J Biochem Mol Toxicol 37:e23383 (2023). PubMed: 37158446
- Gu Z et al. Inhibition of MicroRNA miR-101-3p on prostate cancer progression by regulating Cullin 4B (CUL4B) and PI3K/AKT/mTOR signaling pathways. Bioengineered 12:4719-4735 (2021). PubMed: 34338146
- Homma K et al. Genome-wide siRNA screening reveals that DCAF4-mediated ubiquitination of optineurin stimulates autophagic degradation of Cu,Zn-superoxide dismutase. J Biol Chem 295:3148-3158 (2020). PubMed: 32014991
- Hou L et al. Restriction of hepatitis B virus replication by c-Abl-induced proteasomal degradation of the viral polymerase. Sci Adv 5:eaau7130 (2019). PubMed: 30775435
- Füzesi-Levi MG et al. CSNAP, the smallest CSN subunit, modulates proteostasis through cullin-RING ubiquitin ligases. Cell Death Differ N/A:N/A (2019). PubMed: 31367012
- Song T et al. CRL4 antagonizes SCFFbxo7-mediated turnover of cereblon and BK channel to regulate learning and memory. PLoS Genet 14:e1007165 (2018). PubMed: 29370161
- Wang YL et al. The E3 Ubiquitin Ligase CRL4 Regulates Proliferation and Progression Through Meiosis in Chinese Mitten Crab Eriocheir sinensis. Biol Reprod 94:65 (2016). PubMed: 26864195