Anti-CTRP4抗体(ab36871)
Key features and details
- Rabbit polyclonal to CTRP4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat
- Isotype: IgG
概述
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产品名称
Anti-CTRP4抗体 -
描述
兔多克隆抗体to CTRP4 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Rat -
免疫原
A 16 amino acid peptide from near the carboxy terminus of human CTRP4.
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阳性对照
- Rat brain cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab36871于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).
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| IHC-P |
Use a concentration of 10 µg/ml.
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| ICC/IF |
Use a concentration of 20 µg/ml.
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| 说明 |
|---|
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WB
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa). |
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IHC-P
Use a concentration of 10 µg/ml. |
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ICC/IF
Use a concentration of 20 µg/ml. |
靶标
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功能
May act as a pro-inflammatory cytokine, that induces the activation of NF-kappa-B signaling pathway and up-regulates IL6 production. -
组织特异性
Widely expressed at low levels. -
序列相似性
Contains 2 C1q domains. -
细胞定位
Secreted. - Information by UniProt
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别名
- C1q and tumor necrosis factor related protein 4 antibody
- C1QT4_HUMAN antibody
- C1qtnf4 antibody
see all
图片
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Western blot - Anti-CTRP4 antibody (ab36871)Lane 1 : Anti-CTRP4 antibody (ab36871) at 1 µg/ml
Lane 2 : Anti-CTRP4 antibody (ab36871) at 2 µg/ml
Lane 3 : Anti-CTRP4 antibody (ab36871) at 4 µg/ml
All lanes : Rat brain cell lysate
Lysates/proteins at 15 µg/ml per lane.
Secondary
All lanes : anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size: 37 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Lanes read from left to right 1, 2 and 3. These proteins are often highly modified post-translationally and may migrate in SDS-PAGE at positions other than their predicted size. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTRP4 antibody (ab36871)ab36891 at 10µg/ml staining CTRP4 in rat brain by IHC
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Immunocytochemistry/ Immunofluorescence - Anti-CTRP4 antibody (ab36871)Immunofluorescence of CTRP4 in Rat Brain cells using ab36871 at 20 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab36871 被引用在 3 文献中.
- Li Q et al. Ctrp4, a new adipokine, promotes the differentiation of osteoblasts. Biochem Biophys Res Commun 512:224-229 (2019). PubMed: 30885436
- Bando H et al. CXCR4 regulates Plasmodium development in mouse and human hepatocytes. J Exp Med 216:1733-1748 (2019). PubMed: 31189656
- Byerly MS et al. C1q/TNF-related protein 4 (CTRP4) is a unique secreted protein with two tandem C1q domains that functions in the hypothalamus to modulate food intake and body weight. J Biol Chem 289:4055-69 (2014). WB ; Mouse . PubMed: 24366864