重组Anti-CTNNBL1抗体[EP2669Y] (ab76243)

Flow cytometry analysis showing positive staining in HeLa cells using ab76243 at 1:20 dilution, 5 µg/mL.

Cells were fixed with 100μl 4% PFA for 10 min at RT, then permeabilized with 100μl 90% methanol for 30 min (-20° C) followed by blocking with 10% goat serum for 1h at room temperature. Incubate ab76243 for 30 min at room temperature (RT) and then incubate Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) for 30 min at room temperature.

HeLa cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab76243 at 1:50 was incubated overnight at 4° C, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution at RT for 45 min. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889) at 1/200 dilution, was co-incubated with ab76243 overnight at 4° C. Nucleus were visualized using DAPI.

Immunohistochemical analysis of human breast tissue staining CTNNBL1 with ab76243 at 1/100 dilution.

The human breast sections were performed by LeicaDS9800 (Bond™ Polymer Refine Detection). The sections were using Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins, then labeling ab76243 at 1/100 dilution for 30 mins at room temperature. Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Blocking and diluting buffer and concentration: 5% NFDM/TBST,

Primary antibody: incubated for 1h at room temperature.

Secondary antibody: incubated for 1h at 1:20,000 at room temperature (ab97051)