重组Anti-Ctip1/BCL-11A抗体[123] (ab119275)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] (ab119275)](https://www.abcam.cn/ps/products/119/ab119275/Images/ab119275-368225-antictip1bcl11aantibody123immunohistochemistryspleenrat.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] (ab119275)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [123] to Ctip1/BCL-11A
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-Ctip1/BCL-11A抗体[123]
参阅全部 Ctip1/BCL-11A 一抗 -
描述
小鼠单克隆抗体[123] to Ctip1/BCL-11A -
宿主
Mouse -
经测试应用
适用于: IHC-Pmore details
不适用于: Flow Cyt,ICC/IF,IP or WB -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human tonsil and breast carcinoma tissue; mouse cerebrum tissue; rat spleen tissue.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
123 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119275于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 0.526 - 5.26 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Use at 5.26µg/ml for human and 0.526µg/ml for mouse and rat. |
| 说明 |
|---|
|
IHC-P
Use a concentration of 0.526 - 5.26 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Use at 5.26µg/ml for human and 0.526µg/ml for mouse and rat. |
靶标
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功能
Functions as a myeloid and B-cell proto-oncogene. May play important roles in leukemogenesis and hematopoiesis. An essential factor in lymphopoiesis, is required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1. -
组织特异性
Expressed at high levels in brain, spleen thymus, bone marrow and testis. Expressed in CD34-positive myeloid precursor cells, B-cells, monocytes and megakaryocytes. Expression is tightly regulated during B-cell development. -
疾病相关
Note=Chromosomal aberrations involving BCL11A may be a cause of lymphoid malignancies. Translocation t(2;14)(p13;q32.3) causes BCL11A deregulation and amplification. -
序列相似性
Contains 6 C2H2-type zinc fingers. -
翻译后修饰
Sumoylated by SUMO1. -
细胞定位
Cytoplasm. Nucleus. Associates with the nuclear body. Colocalizes with SUMO1 and SENP2 in nuclear speckles. - Information by UniProt
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数据库链接
- Entrez Gene: 53335 Human
- Entrez Gene: 14025 Mouse
- Entrez Gene: 305589 Rat
- Omim: 606557 Human
- SwissProt: Q9H165 Human
- SwissProt: Q9QYE3 Mouse
- Unigene: 370549 Human
- Unigene: 441102 Mouse
see all -
别名
- 2810047E18Rik antibody
- B cell CLL/lymphoma 11A (zinc finger protein) antibody
- B cell CLL/lymphoma 11A (zinc finger protein) isoform 2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] (ab119275)
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Ctip1/BCL-11A with ab119275 at 0.526µg/ml, followed by a ready to use secondary antibody. Nuclear staining on rat spleen is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use secondary antibody.
The section was incubated with ab119275 for 30 mins at room temperature.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] (ab119275)](https://www.abcam.cn/ps/products/119/ab119275/Images/ab119275-368224-antictip1bcl11aantibody123immunohistochemistrytonsilhuman.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] (ab119275)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Ctip1/BCL-11A with ab119275 at 5.26µg/ml, followed by a ready to use secondary antibody. Nuclear staining on human tonsil is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use secondary antibody.
The section was incubated with ab119275 for 30 mins at room temperature.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] (ab119275)](https://www.abcam.cn/ps/products/119/ab119275/Images/ab119275-368222-antictip1bcl11aantibody123immunohistochemistrycerebrummouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] (ab119275)Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Ctip1/BCL-11A with ab119275 at 0.526µg/ml, followed by a ready to use secondary antibody. Nuclear staining on mouse cerebrum is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use secondary antibody.
The section was incubated with ab119275 for 30 mins at room temperature.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] (ab119275)](https://www.abcam.cn/ps/products/119/ab119275/Images/ab119275-368221-antictip1bcl11aantibody123immunohistochemistrybreastcarcinomahuman.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] (ab119275)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling Ctip1/BCL-11A with ab119275 at 5.26µg/ml, followed by a ready to use secondary antibody. Nuclear staining on human breast carcinoma is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use secondary antibody.
The section was incubated with ab119275 for 30 mins at room temperature.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
-
![Anti-Ctip1/BCL-11A antibody [123] (ab119275)](https://www.abcam.cn/ps/products/119/ab119275/Images/ab119275-8-benefits-of-recombinant-antibodies.png)
Anti-Ctip1/BCL-11A antibody [123] (ab119275)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab119275 被引用在 5 文献中.
- Chetaille B et al. Molecular profiling of classical Hodgkin lymphoma tissues uncovers variations in the tumor microenvironment and correlations with EBV infection and outcome. Blood 113:2765-3775 (2009). PubMed: 19096012
- Marafioti T et al. Novel markers of normal and neoplastic human plasmacytoid dendritic cells. Blood 111:3778-92 (2008). PubMed: 18218851
- Liu H et al. Functional studies of BCL11A: characterization of the conserved BCL11A-XL splice variant and its interaction with BCL6 in nuclear paraspeckles of germinal center B cells. Mol Cancer 5:18 (2006). PubMed: 16704730
- Pulford K et al. The BCL11AXL transcription factor: its distribution in normal and malignant tissues and use as a marker for plasmacytoid dendritic cells. Leukemia 20:1439-41 (2006). PubMed: 16710303
- Weniger MA et al. Gains of the proto-oncogene BCL11A and nuclear accumulation of BCL11A(XL) protein are frequent in primary mediastinal B-cell lymphoma. Leukemia 20:1880-2 (2006). PubMed: 16871282