AB274447

重组Anti-CTHRC1抗体[EPR22851-215] - BSA and Azide free (Detector)

Anti-CTHRC1 antibody [EPR22851-215] - BSA and Azide free (Detector)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal CTHRC1 antibody. Carrier free. Suitable for sELISA, IP and reacts with Human samples.

查看别名

UNQ762/PRO1550, CTHRC1, Collagen triple helix repeat-containing protein 1

2 Images

Supplier Data

Immunoprecipitation - Anti-CTHRC1 antibody [EPR22851-215] - BSA and Azide free (Detector) (AB274447)

Immunoprecipitation of CTHRC1 in A-375 cells. Lysates were prepared and immunoprecipitation was performed using 2 μg of ab274447 pre-coupled to Protein A beads. Samples were then washed and processed for western blot.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Immunoprecipitation - Anti-CTHRC1 antibody [EPR22851-215] - BSA and Azide free (Detector) (ab274447) at 2 µg

All lanes:

A-375 cell lysates

Observed band size: 26 kDa

false

Sandwich ELISA - Anti-CTHRC1 antibody [EPR22851-215] - BSA and Azide free (Detector) (AB274447)

sELISA

Supplier Data

Sandwich ELISA - Anti-CTHRC1 antibody [EPR22851-215] - BSA and Azide free (Detector) (AB274447)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab274399).

不同偶联物与剂型 (1)

Biotin

Biotin Anti-CTHRC1 antibody [EPR22851-215] (Detector)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR22851-215

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

IP, sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2 µg", "IP-species-notes": "<p></p>" } } }

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产品详情

ab274447 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human CTHRC1. The recommended pair for sandwich ELISA is:
Capture: ab274446, Human CTHRC1 Capture Antibody (unconjugated)
Detector: ab274447, Human CTHRC1 Detector Antibody (unconjugated)
The reference range value is 93.75 - 6000 pg/ml.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Constituents: PBS

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The CTHRC1 protein also known as Collagen Triple Helix Repeat Containing 1 is a secreted protein with a mass of approximately 28 kDa. It plays a mechanical role in the extracellular matrix by modulating collagen fibrillogenesis and cross-linking. Increased expression of CTHRC1 protein often appears in areas undergoing active remodeling like those around wound healing sites fibrotic lesions and certain tumor tissues. The expression is not uniform across tissues and often corresponds to areas requiring structural reorganization or repair.

Biological function summary

CTHRC1 impacts cell migration and invasion. It associates with several extracellular matrix components influencing cellular response through cytoskeletal reorganization. There is no evidence for its participation as a part of a larger protein complex. The modulation of collagen interactions by CTHRC1 enables it to play a significant role in angiogenesis and tissue repair processes affecting endothelial and fibroblast cell functions.

Pathways

CTHRC1 participates in signaling pathways related to Transforming Growth Factor-beta (TGF-β) and Wnt. In the TGF-β pathway it impacts cellular responses to growth signals enhancing remodeling and repair mechanisms. Through the Wnt signaling pathway CTHRC1 interacts with Dishevelled proteins assisting in regulation and pathway activation related to cell proliferation and migration. These pathways highlight its involvement in important biological processes that influence tissue structure and cell communication.

CTHRC1 has relevance to both cancer progression and fibrotic diseases. Its overexpression is often noted in breast cancer where it drives tumor growth and metastasis interacting with other matrix components. Similarly in fibrosis CTHRC1 contributes to excessive collagen deposition and tissue stiffness. Proteins like TGF-β intersect with CTHRC1 functions further modulating disease pathway outcomes. Understanding CTHRC1's role in these disorders could inform therapeutic targets for modulating matrix dynamics.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

May act as a negative regulator of collagen matrix deposition.

See full target information CTHRC1

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Abcam Product Promise

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详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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重组Anti-CTHRC1抗体[EPR22851-215] - BSA and Azide free (Detector)