重组Anti-CTAG1B抗体[SP349] (ab223498)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] (ab223498)](https://www.abcam.cn/ps/products/223/ab223498/Images/ab223498-7-AntiCTAG1B-antibody-SP349-IHC-Human-gastric-carcinoma.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] (ab223498)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP349] to CTAG1B
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CTAG1B抗体[SP349]
参阅全部 CTAG1B 一抗 -
描述
兔单克隆抗体[SP349] to CTAG1B -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human CTAG1B.
Database link: P78358 -
阳性对照
- IHC-P: Human testis, and Human gastric carcinoma tissue; WB: NY-ESO-1 or LAGE-2 transfected HEK-293 cell lysates.
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常规说明
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
纯度
Protein A/G purified -
克隆
单克隆 -
克隆编号
SP349 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223498于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/100.
|
|
| IHC-P |
1/100.
|
|
| Flow Cyt |
1/100.
|
| 说明 |
|---|
|
WB
1/100. |
|
IHC-P
1/100. |
|
Flow Cyt
1/100. |
靶标
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组织特异性
Expressed in testis and ovary and in a wide variety of cancers. Detected in uterine myometrium. Expressed from 18 weeks until birth in human fetal testis. In the adult testis, is strongly expressed in spermatogonia and in primary spermatocytes, but not in post-meiotic cells or in testicular somatic cells (at protein level). -
序列相似性
Belongs to the CTAG family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 1485 Human
- Entrez Gene: 246100 Human
- Omim: 300156 Human
- SwissProt: P78358 Human
- Unigene: 534310 Human
- Unigene: 729551 Human
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别名
- CTAG1A antibody
- CTAG1B antibody
- Autoimmunogenic cancer/testis antigen NY ESO 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] (ab223498)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human gastric carcinoma tissue sections labeling CTAG1B with Purified ab223498 at 1/100 dilution (3.83 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] (ab223498)](https://www.abcam.cn/ps/products/223/ab223498/Images/ab223498-6-AntiCTAG1B-antibody-SP349-IHC-Human-testis.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] (ab223498)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling CTAG1B with Purified ab223498 at 1/100 dilution (3.83 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Flow Cytometry - Anti-CTAG1B antibody [SP349] (ab223498)](https://www.abcam.cn/ps/products/223/ab223498/Images/ab223498-333805-anti-ctag1b-antibody-sp349-flow-cytometry.jpg)
Flow Cytometry - Anti-CTAG1B antibody [SP349] (ab223498)Flow Cytometry analysis of NCI-H1299 (human lung carcinoma epithelial cell) cells labeling CTAG1B with purified ab223498 at 1:380 dilution (1.01 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] (ab223498)](https://www.abcam.cn/ps/products/223/ab223498/Images/ab223498-282983-anti-ctag1b-antibody-sp349-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] (ab223498)Formalin-fixed, paraffin-embedded human testis tissue stained for CTAG1B using ab223498 at 1/100 dilution in immunohistochemical analysis.
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![Western blot - Anti-CTAG1B antibody [SP349] (ab223498)](https://www.abcam.cn/ps/products/223/ab223498/Images/ab223498-282984-anti-ctag1b-antibody-sp349-western-blot.jpg)
Western blot - Anti-CTAG1B antibody [SP349] (ab223498)All lanes : Anti-CTAG1B antibody [SP349] (ab223498) at 1/100 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) transfected with LAGE-1 cell lysate.
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) CTAG1B isoform1a transfected cell lysate. -
![Flow Cytometry - Anti-CTAG1B antibody [SP349] (ab223498)](https://www.abcam.cn/ps/products/223/ab223498/Images/ab223498-282982-anti-ctag1b-antibody-sp349-flow-cytometry.jpg)
Flow Cytometry - Anti-CTAG1B antibody [SP349] (ab223498)Flow cytometric analysis of ab223498 staining HeLa (human epithelial cell line from cervix adenocarcinoma) cells for CTAG1B (green) at 1/100 dilution compared to negative control of rabbit IgG (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] (ab223498)](https://www.abcam.cn/ps/products/223/ab223498/Images/ab223498-282992-anti-ctag1b-antibody-sp349-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] (ab223498)Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for CTAG1B with ab223498 at 1/100 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab223498 被引用在 1 文献中.
- Magara T et al. Tumor immune microenvironment of cutaneous angiosarcoma with cancer testis antigens and the formation of tertiary lymphoid structures. Front Oncol 13:1106434 (2023). PubMed: 37081973