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重组RabMAb

重组Anti-CT A抗体[EPR3940(2)] (ab109263)

Submit a review Submit a question References (9)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)
  • Immunocytochemistry/ Immunofluorescence - Anti-CT A antibody [EPR3940(2)] (ab109263)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)
  • Western blot - Anti-CT A antibody [EPR3940(2)] (ab109263)
  • Western blot - Anti-CT A antibody [EPR3940(2)] (ab109263)
  • Western blot - Anti-CT A antibody [EPR3940(2)] (ab109263)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)
  • Anti-CT A antibody [EPR3940(2)] (ab109263)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3940(2)] to CT A
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-CT A抗体[EPR3940(2)]
    参阅全部 CT A 一抗

  • 描述

    兔单克隆抗体[EPR3940(2)] to CT A

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP

  • 种属反应性

    与反应: Mouse, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HeLa, HepG2, A549, K562 , Mouse brain, and RAW 264.7 lysates. IHC-P: human colon, human prostatic hyperplasia tissue and mouse pancreas tissues. ICC/IF: HeLa cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab109263于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

1:500 in human tissues / 1:1000 in mouse tissues

See IHC antigen retrieval protocols.
ICC/IF
1/50.
说明
WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

1:500 in human tissues / 1:1000 in mouse tissues

See IHC antigen retrieval protocols.
ICC/IF
1/50.
应用说明
Is unsuitable for Flow Cyt or IP.

靶标

  • 功能

    Controls phosphatidylcholine synthesis.

  • 通路

    Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 1/2.

  • 序列相似性

    Belongs to the cytidylyltransferase family.

  • 翻译后修饰

    The serine residues of the C-terminus are phosphorylated. The inactive soluble form is stabilized by phosphorylation, the active membrane bound form is promoted by anionic lipids or diacylglycerol, and is stabilized by dephosphorylation.

  • 细胞定位

    Cytoplasm > cytosol. Membrane. It can interconvert between an inactive cytosolic form and an active membrane-bound form.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CCT A antibody
      • CCT alpha antibody
      • CCT-alpha antibody
      • Choline phosphate cytidylyltransferase A antibody
      • Choline-phosphate cytidylyltransferase A antibody
      • CT A antibody
      • CT antibody
      • CTP:phosphocholine cytidylyltransferase A antibody
      • CTPCT antibody
      • PCY1A_HUMAN antibody
      • PCYT1 antibody
      • Pcyt1a antibody
      • Phosphate cytidylyltransferase 1 choline alpha antibody
      • Phosphate cytidylyltransferase 1 choline alpha isoform antibody
      • Phosphorylcholine transferase A antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse pancreas tissue sections labeling CT A with Purified ab109263 at 1:1000 dilution (0.53 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunocytochemistry/ Immunofluorescence - Anti-CT A antibody [EPR3940(2)] (ab109263)
      Immunocytochemistry/ Immunofluorescence - Anti-CT A antibody [EPR3940(2)] (ab109263)
      Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling CT A with Purified ab109263 at 1:50 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling CT A with Purified ab109263 at 1:500 dilution (1.06 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Western blot - Anti-CT A antibody [EPR3940(2)] (ab109263)
      Western blot - Anti-CT A antibody [EPR3940(2)] (ab109263)
      Anti-CT A antibody [EPR3940(2)] (ab109263) at 1/5000 dilution (Purified) + RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 42 kDa
      Observed band size: 42 kDa

    • Western blot - Anti-CT A antibody [EPR3940(2)] (ab109263)
      Western blot - Anti-CT A antibody [EPR3940(2)] (ab109263)
      All lanes : Anti-CT A antibody [EPR3940(2)] (ab109263) at 1/5000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : Mouse brain lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 42 kDa
      Observed band size: 42 kDa

    • Western blot - Anti-CT A antibody [EPR3940(2)] (ab109263)
      Western blot - Anti-CT A antibody [EPR3940(2)] (ab109263)
      All lanes : Anti-CT A antibody [EPR3940(2)] (ab109263) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 42 kDa
      Observed band size: 42 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)

      ab109263, at a 1/50 dilution, staining CT A in paraffin embedded Human colon tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)

      ab109263, at a 1/50 dilution, staining CT A in paraffin embedded Human prostatic hyperplasia by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-CT A antibody [EPR3940(2)] (ab109263)
      Anti-CT A antibody [EPR3940(2)] (ab109263)

    文献 (9)

    发表研究结果有使用 ab109263？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab109263 被引用在 9 文献中.

    • Östlund C  et al. Hepatocytes Deficient in Nuclear Envelope Protein Lamina-associated Polypeptide 1 are an Ideal Mammalian System to Study Intranuclear Lipid Droplets. J Lipid Res 63:100277 (2022). PubMed: 36100089
    • Christensen KE  et al. Mild Choline Deficiency and MTHFD1 Synthetase Deficiency Interact to Increase Incidence of Developmental Delays and Defects in Mice. Nutrients 14:N/A (2021). PubMed: 35011003
    • Yang J  et al. CCT a is a novel biomarker for diagnosis of laryngeal squamous cell cancer. Sci Rep 9:11823 (2019). PubMed: 31413263
    • Petkevicius K  et al. Accelerated phosphatidylcholine turnover in macrophages promotes adipose tissue inflammation in obesity. Elife 8:N/A (2019). PubMed: 31418690
    • Huang Q  et al. Stimulated phospholipid synthesis is key for hepatitis B virus replications. Sci Rep 9:12989 (2019). PubMed: 31506451
    • Chen S  et al. In vitro fertilization alters phospholipid profiles in mouse placenta. J Assist Reprod Genet N/A:N/A (2019). PubMed: 30610659
    • Viswanath P  et al. 2-Hydroxyglutarate-Mediated Autophagy of the Endoplasmic Reticulum Leads to an Unusual Downregulation of Phospholipid Biosynthesis in Mutant IDH1 Gliomas. Cancer Res 78:2290-2304 (2018). PubMed: 29358170
    • Haider A  et al. PCYT1A Regulates Phosphatidylcholine Homeostasis from the Inner Nuclear Membrane in Response to Membrane Stored Curvature Elastic Stress. Dev Cell 45:481-495.e8 (2018). PubMed: 29754800
    • Payne F  et al. Mutations disrupting the Kennedy phosphatidylcholine pathway in humans with congenital lipodystrophy and fatty liver disease. Proc Natl Acad Sci U S A 111:8901-6 (2014). WB ; Human . PubMed: 24889630

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