Anti-CSTF3抗体(ab72299)
Key features and details
- Rabbit polyclonal to CSTF3
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CSTF3抗体
参阅全部 CSTF3 一抗 -
描述
兔多克隆抗体to CSTF3 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Horse, Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human CSTF3 (N terminal). Corresponding to a region between residue 1 and 50 of human CSTF3 (NP_001317.1)
Database link: Q12996 -
阳性对照
- Whole cell lysates from HeLa and 293T cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72299于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
1/2000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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相关性
CSTF3 (cleavage stimulation factor subunit 3) is required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs and is part of the CSTF complex. The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). CSTF3 functions as a homodimer and interacts directly with CSTF1 and CSTF2. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 1479 Human
- Entrez Gene: 100174283 Orangutan
- Omim: 600367 Human
- SwissProt: Q12996 Human
- SwissProt: Q5RDW9 Orangutan
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别名
- CF 1 77 kDa subunit antibody
- Cleavage stimulation factor 3' pre RNA subunit 3 antibody
- Cleavage stimulation factor 77 kDa subunit antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSTF3 antibody (ab72299)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling CSTF3 with ab72299 at 1/200 (1µg/ml). Detection: DAB.
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Western blot - Anti-CSTF3 antibody (ab72299)All lanes : Anti-CSTF3 antibody (ab72299) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 83 kDa
Observed band size: 83 kDa
Additional bands at: 150 kDa, 31 kDa, 50 kDa, 60 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Anti-CSTF3 antibody (ab72299)Detection of Human CSTF3 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72299 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab72299 尚未被引用在任何文献中。