AB72297

Anti-CstF-64抗体

Anti-CstF-64 antibody

BOND RX™ Validated
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(4 Publications)

Rabbit Polyclonal CstF-64 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human Cleavage stimulation factor subunit 2 aa 350-450.

查看别名

Cleavage stimulation factor subunit 2, CF-1 64 kDa subunit, Cleavage stimulation factor 64 kDa subunit, CSTF 64 kDa subunit, CstF-64, CSTF2

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CstF-64 antibody (AB72297)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CstF-64 antibody (AB72297)

IHC image of CstF-64 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72297, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CstF-64 antibody (AB72297)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling Cleavage stimulation factor 2 with ab72297 at 1/1000 (1µg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-CstF-64 antibody (AB72297)

Detection of human CstF-64 by Immunoprecipitation using whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), and ab72297 at 3 μg/mg lysate. Subsequent WB detection was performed using ab72297 at 0.1 μg/ml. Detection was by chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-CstF-64 antibody (ab72297)

Predicted band size: 61 kDa

false

Unknown

Western blot - Anti-CstF-64 antibody (AB72297)

All lanes:

Western blot - Anti-CstF-64 antibody (ab72297) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 61 kDa

Observed band size: 55 kDa,68 kDa

true

Exposure time: 10s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, WB, IP

applications

免疫原

Synthetic Peptide within Human Cleavage stimulation factor subunit 2 aa 350-450. The exact immunogen used to generate this antibody is proprietary information.

P33240

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab72297 was affinity purified using an epitope specific to CstF-64 immobilized on solid support.

存储溶液

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Do Not Freeze

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs.

See full target information Cleavage stimulation factor subunit 2

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Functional & integrative genomics 25:103 PubMed40381037

2025

3'UTR RNA editing driven by ADAR1 modulates MDM2 expression in breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Elanur Almeric,Deniz Karagozoglu,Mustafa Cicek,Didem Naz Dioken,Huseyin Avni Tac,Esra Cicek,Busra Aytul Kirim,Irmak Gurcuoglu,Osman Ugur Sezerman,Nurhan Ozlu,Ayse Elif Erson-Bensan

Scientific reports 14:10987 PubMed38745101

2024

Alternative 3'UTR expression induced by T cell activation is regulated in a temporal and signal dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Davia Blake,Matthew R Gazzara,Isabel Breuer,Max Ferretti,Kristen W Lynch

Molecular therapy. Nucleic acids 14:251-261 PubMed30641477

2019

Interference of DNAJB6/MRJ Isoform Switch by Morpholino Inhibits Replication of HIV-1 and RSV.

Applications

Unspecified application

Species

Unspecified reactive species

Shih-Han Ko,Yi-Jen Liau,Ya-Hui Chi,Mei-Ju Lai,Yu-Ping Chiang,Chun-Yi Lu,Luan-Yin Chang,Woan-Yuh Tarn,Li-Min Huang

Biochimica et biophysica acta 1800:977-85 PubMed20541591

2010

Regulation of post-translational protein arginine methylation during HeLa cell cycle.

Applications

Species

Human

Chongtae Kim,Yongchul Lim,Byong Chul Yoo,Nam Hee Won,Sangduk Kim,Gieun Kim

View all publications

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Abcam Product Promise

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详情请参阅我们的条款与条件。

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For licensing inquiries, please contact partnerships@abcam.com

Anti-CstF-64抗体

Anti-CstF-64 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CstF-64 antibody (AB72297)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CstF-64 antibody (AB72297)

Immunoprecipitation - Anti-CstF-64 antibody (AB72297)

Western blot - Anti-CstF-64 antibody (AB72297)