重组Anti-CstF-50抗体[EPR12332] (ab173288)
![Western blot - Anti-CstF-50 antibody [EPR12332] (ab173288)](https://www.abcam.cn/ps/products/173/ab173288/Images/ab173288-196670-ab173288WB1.jpg "Western blot - Anti-CstF-50 antibody [EPR12332] (ab173288)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12332] to CstF-50
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CstF-50抗体[EPR12332]
参阅全部 CstF-50 一抗 -
描述
兔单克隆抗体[EPR12332] to CstF-50 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Recombinant fragment within Human CstF-50. The exact sequence is proprietary.
Database link: Q05048 -
阳性对照
- 293T, HeLa, A549 and K562 cell lysates; Human cervical carcinoma and testis tissues; A549 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR12332 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab173288于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/50 - 1/100.
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| WB |
1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
|
| 说明 |
|---|
|
ICC/IF
1/50 - 1/100. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 48 kDa. |
靶标
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功能
One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. May be responsible for the interaction of CSTF with other factors to form a stable complex on the pre-mRNA. -
序列相似性
Contains 6 WD repeats. -
结构域
The WD6 domain is required for interaction with BARD1. WD domains are responsible for interaction with CSTF3.
N-terminus mediates homodimerization. -
翻译后修饰
The N-terminus is blocked. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 1477 Human
- Entrez Gene: 67337 Mouse
- Entrez Gene: 311670 Rat
- Omim: 600369 Human
- SwissProt: Q05048 Human
- SwissProt: Q99LC2 Mouse
- SwissProt: Q5BJQ6 Rat
- Unigene: 172865 Human
see all -
别名
- CF 1 50 kDa subunit antibody
- CF-1 50 kDa subunit antibody
- Cleavage stimulation factor 50 kDa subunit antibody
see all
图片
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Western blot - Anti-CstF-50 antibody [EPR12332] (ab173288)All lanes : Anti-CstF-50 antibody [EPR12332] (ab173288) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A549 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 48 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CstF-50 antibody [EPR12332] (ab173288)](https://www.abcam.cn/ps/products/173/ab173288/Images/ab173288-196671-ab173288IHC11.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CstF-50 antibody [EPR12332] (ab173288)Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling CstF-50 with ab173288 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CstF-50 antibody [EPR12332] (ab173288)](https://www.abcam.cn/ps/products/173/ab173288/Images/ab173288-196672-ab173288IHC21.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CstF-50 antibody [EPR12332] (ab173288)Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling CstF-50 with ab173288 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-CstF-50 antibody [EPR12332] (ab173288)](https://www.abcam.cn/ps/products/173/ab173288/Images/ab173288-196673-ab173288ICC.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CstF-50 antibody [EPR12332] (ab173288)Immunofluorescent analysis of A549 cells labeling CstF-50 with ab173288 at 1/50 dilution.
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![Anti-CstF-50 antibody [EPR12332] (ab173288)](https://www.abcam.cn/ps/products/173/ab173288/Images/ab173288-5-benefits-of-recombinant-antibodies.png)
Anti-CstF-50 antibody [EPR12332] (ab173288)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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