AB172952

重组Anti-CSRP3抗体[EPR12615(B)]

Anti-CSRP3 antibody [EPR12615(B)]

RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal CSRP3 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 3 publications.

查看别名

CLP, MLP, CSRP3, Cysteine and glycine-rich protein 3, Cardiac LIM protein, Cysteine-rich protein 3, Muscle LIM protein, CRP3

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (AB172952)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (AB172952)

ab172952 showing -ve staining in Human normal lung tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (AB172952)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (AB172952)

ab172952 showing -ve staining in Human normal brain tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (AB172952)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (AB172952)

ab172952 showing -ve staining in Human normal liver tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (AB172952)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (AB172952)

Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling CSRP3 with ab172592 at 1/1000 dilution.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (AB172952)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (AB172952)

ab172952 showing -ve staining in Human normal tonsil tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (AB172952)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (AB172952)

Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling CSRP3 with ab172952 at 1/1000 dilution.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Immunoprecipitation - Anti-CSRP3 antibody [EPR12615(B)] (AB172952)

IP

Supplier Data

Immunoprecipitation - Anti-CSRP3 antibody [EPR12615(B)] (AB172952)

Western blot analysis on immunoprecipitation pellet from (1) fetal heart lysate or (2) 1X PBS (negative control) using ab172952, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-CSRP3 antibody [EPR12615(B)] (ab172952)

Predicted band size: 21 kDa

false

Western blot - Anti-CSRP3 antibody [EPR12615(B)] (AB172952)

WB

Supplier Data

Western blot - Anti-CSRP3 antibody [EPR12615(B)] (AB172952)

All lanes:

Western blot - Anti-CSRP3 antibody [EPR12615(B)] (ab172952) at 1/1000 dilution

Lane 1:

Fetal heart at 10 µg

Lane 2:

Human skeletal muscle at 10 µg

Lane 3:

Fetal muscle at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit antibody at 1/2000 dilution

Predicted band size: 21 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-CSRP3 antibody [EPR12615(B)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12615(B)

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB, IP, IHC-P

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

推荐的短期储存时间

1-2 weeks

推荐的短期储存条件

+4°C

推荐的长期储存条件

-20°C

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Positive regulator of myogenesis. Acts as a cofactor for myogenic bHLH transcription factors such as MYOD1, and probably MYOG and MYF6. Enhances the DNA-binding activity of the MYOD1 : TCF3 isoform E47 complex and may promote formation of a functional MYOD1 : TCF3 isoform E47 : MEF2A complex involved in myogenesis (By similarity). Plays a crucial and specific role in the organization of cytosolic structures in cardiomyocytes. Could play a role in mechanical stretch sensing. May be a scaffold protein that promotes the assembly of interacting proteins at Z-line structures. It is essential for calcineurin anchorage to the Z line. Required for stress-induced calcineurin-NFAT activation (By similarity). The role in regulation of cytoskeleton dynamics by association with CFL2 is reported conflictingly : Shown to enhance CFL2-mediated F-actin depolymerization dependent on the CSRP3 : CFL2 molecular ratio, and also shown to reduce the ability of CLF1 and CFL2 to enhance actin depolymerization (PubMed : 19752190, PubMed : 24934443). Proposed to contribute to the maintenance of muscle cell integrity through an actin-based mechanism. Can directly bind to actin filaments, cross-link actin filaments into bundles without polarity selectivity and protect them from dilution- and cofilin-mediated depolymerization; the function seems to involve its self-association (PubMed : 24934443). In vitro can inhibit PKC/PRKCA activity (PubMed : 27353086). Proposed to be involved in cardiac stress signaling by down-regulating excessive PKC/PRKCA signaling (By similarity).. Isoform 2. May play a role in early sarcomere organization. Overexpression in myotubes negatively regulates myotube differentiation. By association with isoform 1 and thus changing the CSRP3 isoform 1 : CFL2 stoichiometry is proposed to down-regulate CFL2-mediated F-actin depolymerization.

See full target information CSRP3

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 17:5332-5343 PubMed40821094

2025

Endothelial progenitor cell-derived microvesicles therapy relieves myocardial infarction symptoms by altering left ventricular protein expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yanling Song,Shuai Wang,Huade Mai,Minghui Chen,Yunyun Lin,Huajun Wu,Shenhong Gu

Reviews in cardiovascular medicine 23:247 PubMed39076905

2022

Construction of Prediction Model for Atrial Fibrillation with Valvular Heart Disease Based on Machine Learning.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaoqiao Li,Shenghong Lei,Xueshan Luo,Jintao He,Yuan Fang,Hui Yang,Yang Liu,Chun-Yu Deng,Shulin Wu,Yu-Mei Xue,Fang Rao

Nature communications 13:1109 PubMed35232997

2022

ADAP1 promotes latent HIV-1 reactivation by selectively tuning KRAS-ERK-AP-1 T cell signaling-transcriptional axis.

Applications

Unspecified application

Species

Unspecified reactive species

Nora-Guadalupe P Ramirez,Jeon Lee,Yue Zheng,Lianbo Li,Bryce Dennis,Didi Chen,Ashwini Challa,Vicente Planelles,Kenneth D Westover,Neal M Alto,Iván D'Orso

View all publications

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