Anti-CSNK2A2抗体(ab10474)
Key features and details
- Rabbit polyclonal to CSNK2A2
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
-
产品名称
Anti-CSNK2A2抗体
参阅全部 CSNK2A2 一抗 -
描述
兔多克隆抗体to CSNK2A2 -
宿主
Rabbit -
特异性
Reacts with the alpha prime subunit. -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant -
免疫原
Synthetic peptide corresponding to Human CSNK2A2 aa 300 to the C-terminus.
Database link: 1459 -
阳性对照
- WB: HeLa, HEK293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
-
常规说明
CKII is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The gene for this protein is found on chromosome 20, but a related transcribed pseudogene is found on chromosome 11. Three transcript variants have been found, encoding two different isoforms. Protein kinase CKII is recognized as one of the key cellular signals for cell growth and proliferation; it also has an important role in apoptosis.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
Antibodies were affinity purified using the peptide immobilized on solid support. -
Primary antibody说明
CKII is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The gene for this protein is found on chromosome 20, but a related transcribed pseudogene is found on chromosome 11. Three transcript variants have been found, encoding two different isoforms. Protein kinase CKII is recognized as one of the key cellular signals for cell growth and proliferation; it also has an important role in apoptosis. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10474于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (4) |
1/1000 - 1/5000. Detects a band of approximately 39 kDa (predicted molecular weight: 41 kDa).
|
IP | (1) |
Use at 2-10 µg/mg of lysate.
|
说明 |
---|
WB
1/1000 - 1/5000. Detects a band of approximately 39 kDa (predicted molecular weight: 41 kDa). |
IP
Use at 2-10 µg/mg of lysate. |
靶标
-
功能
Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins. During viral infection, phosphorylates various proteins involved in the viral life cycles of EBV, HSV, HBV, HCV, HIV, CMV and HPV. -
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.
Contains 1 protein kinase domain. - Information by UniProt
-
数据库链接
- Entrez Gene: 282420 Cow
- Entrez Gene: 1459 Human
- Entrez Gene: 13000 Mouse
- Omim: 115442 Human
- SwissProt: P20427 Cow
- SwissProt: P19784 Human
- SwissProt: O54833 Mouse
- Unigene: 82201 Human
see all -
别名
- Casein kinase 2 alpha prime polypeptide antibody
- Casein kinase II alpha' chain antibody
- Casein kinase II subunit alpha' antibody
see all
图片
-
Detection of human and mouse CSNK2A1 by western blot.
Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer.
Antibody: Affinity purified rabbit anti-CSNK2A1 antibody A300-199A (lot A300-199A-5) used for WB at 0.1 µg/ml.
Detection: Chemiluminescence -
CSNK2A2 was immunoprecipitated from 1mg of HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate with ab10474 at 6 µg/ml. Western blot was performed from the immunoprecipitate using ab10474 at 1 µg/ml.
Lane 1: ab10474 IP in HeLa whole cell lysate.
Lane 2: Ctrl IgG instead of ab10474.
-
Detection of human CSNK2A2 by Western blot and immunoprecipitation.
A. RIPA extract (30 mg) from HeLa cells treated with CSNK2A2 siRNA or vimentin siRNA (v).
B. RIPA extract (30 mg) from HeLa cells treated with CSNK2A2, CSNK2A2 prime, or vimentin (v) siRNA.
C. RIPA extract (0.5mg) from HeLa cells.
Antibody: ab10474 used at 0.33 (A) or 0.2 (B) mg/ml for WB or 5 mg/0.5 mg lysate (C) for IP.
Detection of human CSNK2A2 by Western blot and immunoprecipitation. A. RIPA extract (30 mg) from HeLa cells treated with CSNK2A2 siRNA or vimentin siRNA (v). B. RIPA extract (30 mg) from HeLa cells treated with CSNK2A2, CSNK2A2, or vimentin (v) siRNA. C. RIPA extract (0.5mg) from HeLa cells. Antibody: ab10474 used at 0.33 (A) or 0.2 (B) mg/ml for WB or 5 ug/0.5 mg lysate (C) for IP.
-
All lanes : Anti-CSNK2A2 antibody (ab10474) at 1/500 dilution
Lane 1 : Whole cell lysate prepared from alpha prime knock out MEFs
Lane 2 : Whole cell lysate prepared from alpha prime heterozygous MEFs
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti rabbit IgG-HRP at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 30 minutes
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (5)
ab10474 被引用在 5 文献中.
- Beale AD et al. Casein Kinase 1 Underlies Temperature Compensation of Circadian Rhythms in Human Red Blood Cells. J Biol Rhythms 34:144-153 (2019). PubMed: 30898060
- Hoque A et al. Mitochondrial fission protein Drp1 inhibition promotes cardiac mesodermal differentiation of human pluripotent stem cells. Cell Death Discov 4:39 (2018). WB . PubMed: 29531836
- Tsuchiya Y et al. The casein kinase 2-nrf1 axis controls the clearance of ubiquitinated proteins by regulating proteasome gene expression. Mol Cell Biol 33:3461-72 (2013). PubMed: 23816881
- McFarland TP et al. The cytosolic protein kinase CK2 phosphorylates cardiac calsequestrin in intact cells. Mol Cell Biochem 353:81-91 (2011). WB ; Human . PubMed: 21431367
- Jacks KA & Koch CA Differential regulation of mitogen- and stress-activated protein kinase-1 and -2 (MSK1 and MSK2) by CK2 following UV radiation. J Biol Chem 285:1661-70 (2010). WB . PubMed: 19933278