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AB10474

Anti-CSNK2A2 抗体

Anti-CSNK2A2 antibody

5

(6 Reviews)

|

(5 Publications)

Rabbit Polyclonal CSNK2A2 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human CSNK2A2 aa 300 to C-terminus.

查看别名

CK2A2, CSNK2A2, Casein kinase II subunit alpha', CK II alpha'

4 Images
Immunoprecipitation - Anti-CSNK2A2 antibody (AB10474)
  • IP

Supplier Data

Immunoprecipitation - Anti-CSNK2A2 antibody (AB10474)

CSNK2A2 was immunoprecipitated from 1mg of HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate with ab10474 at 6 μg/ml. Western blot was performed from the immunoprecipitate using ab10474 at 1 μg/ml.

Lane 1 : ab10474 IP in HeLa whole cell lysate.

Lane 2 : Ctrl IgG instead of ab10474.

All lanes:

Immunoprecipitation - Anti-CSNK2A2 antibody (ab10474)

Predicted band size: 41 kDa

false

Exposure time: 10s

Western blot - Anti-CSNK2A2 antibody (AB10474)
  • WB

Unknown

Western blot - Anti-CSNK2A2 antibody (AB10474)

Detection of human and mouse CSNK2A1 by western blot.
Samples : Whole cell lysate (50 μg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer.
Antibody : Affinity purified rabbit anti-CSNK2A1 antibody A300-199A (lot A300-199A-5) used for WB at 0.1 μg/ml.
Detection : Chemiluminescence

All lanes:

Western blot - Anti-CSNK2A2 antibody (ab10474)

Predicted band size: 41 kDa

false

Exposure time: 30s

Western blot - Anti-CSNK2A2 antibody (AB10474)
  • WB

Unknown

Western blot - Anti-CSNK2A2 antibody (AB10474)

Detection of human CSNK2A2 by Western blot and immunoprecipitation.

A. RIPA extract (30 mg) from HeLa cells treated with CSNK2A2 siRNA or vimentin siRNA (v).

B. RIPA extract (30 mg) from HeLa cells treated with CSNK2A2, CSNK2A2 prime, or vimentin (v) siRNA.

C. RIPA extract (0.5mg) from HeLa cells.

Antibody : ab10474 used at 0.33 (A) or 0.2 (B) mg/ml for WB or 5 mg/0.5 mg lysate (C) for IP.

Detection of human CSNK2A2 by Western blot and immunoprecipitation. A. RIPA extract (30 mg) from HeLa cells treated with CSNK2A2 siRNA or vimentin siRNA (v). B. RIPA extract (30 mg) from HeLa cells treated with CSNK2A2, CSNK2A2, or vimentin (v) siRNA. C. RIPA extract (0.5mg) from HeLa cells. Antibody : ab10474 used at 0.33 (A) or 0.2 (B) mg/ml for WB or 5 ug/0.5 mg lysate (C) for IP.

All lanes:

Western blot - Anti-CSNK2A2 antibody (ab10474)

Predicted band size: 41 kDa

false

Western blot - Anti-CSNK2A2 antibody (AB10474)
  • WB

AbReview25167****

Western blot - Anti-CSNK2A2 antibody (AB10474)

All lanes:

Western blot - Anti-CSNK2A2 antibody (ab10474) at 1/500 dilution

Lane 1:

Whole cell lysate prepared from alpha prime knock out MEFs at 40 µg

Lane 2:

Whole cell lysate prepared from alpha prime heterozygous MEFs at 40 µg

Secondary

All lanes:

Goat anti rabbit IgG-HRP at 1/5000 dilution

Predicted band size: 41 kDa

Observed band size: 37 kDa

true

Exposure time: 30min

Image courtesy of an anonymous Abreview.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Human

应用

IP, WB

applications

免疫原

Synthetic Peptide within Human CSNK2A2 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P19784

特异性

Reacts with the alpha prime subunit.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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产品详情

CKII is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The gene for this protein is found on chromosome 20, but a related transcribed pseudogene is found on chromosome 11. Three transcript variants have been found, encoding two different isoforms. Protein kinase CKII is recognized as one of the key cellular signals for cell growth and proliferation; it also has an important role in apoptosis.
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
Antibodies were affinity purified using the peptide immobilized on solid support.
存储溶液
pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine (PubMed : 11239457, PubMed : 11704824, PubMed : 16193064, PubMed : 30898438). Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection (PubMed : 11704824, PubMed : 16193064, PubMed : 30898438). May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response (PubMed : 12631575, PubMed : 19387551, PubMed : 19387552). During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage (PubMed : 12631575, PubMed : 19387551, PubMed : 19387552). Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation (PubMed : 11239457). Phosphorylates a number of DNA repair proteins in response to DNA damage, such as MDC1, RAD9A, RAD51 and HTATSF1, promoting their recruitment to DNA damage sites (PubMed : 20545769, PubMed : 21482717, PubMed : 22325354, PubMed : 26811421, PubMed : 30898438, PubMed : 35597237). Can also negatively regulate apoptosis (PubMed : 19387551, PubMed : 19387552). Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3 (PubMed : 12631575, PubMed : 19387551, PubMed : 19387552). Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8 (PubMed : 12631575, PubMed : 19387551, PubMed : 19387552). Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV (PubMed : 12631575, PubMed : 19387551, PubMed : 19387552). Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB (PubMed : 12631575, PubMed : 19387551, PubMed : 19387552). Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function (PubMed : 19387550). Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1 (PubMed : 19387549). Acts as an ectokinase that phosphorylates several extracellular proteins (PubMed : 12631575, PubMed : 19387551, PubMed : 19387552). During viral infection, phosphorylates various proteins involved in the viral life cycles of EBV, HSV, HBV, HCV, HIV, CMV and HPV (PubMed : 12631575, PubMed : 19387551, PubMed : 19387552). May phosphorylate histone H2A on 'Ser-1' (PubMed : 38334665).
See full target information CSNK2A2
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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of biological rhythms 34:144-153 PubMed30898060

2019

Casein Kinase 1 Underlies Temperature Compensation of Circadian Rhythms in Human Red Blood Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew D Beale,Emily Kruchek,Stephen J Kitcatt,Erin A Henslee,Jack S W Parry,Gabriella Braun,Rita Jabr,Malcolm von Schantz,John S O'Neill,Fatima H Labeed

Cell death discovery 4:39 PubMed29531836

2018

Mitochondrial fission protein Drp1 inhibition promotes cardiac mesodermal differentiation of human pluripotent stem cells.

Applications

WB

Species

Unspecified reactive species

Ashfaqul Hoque,Priyadharshini Sivakumaran,Simon T Bond,Naomi X Y Ling,Anne M Kong,John W Scott,Nadeeka Bandara,Damián Hernández,Guei-Sheung Liu,Raymond C B Wong,Michael T Ryan,Derek J Hausenloy,Bruce E Kemp,Jonathan S Oakhill,Brian G Drew,Alice Pébay,Shiang Y Lim

Molecular and cellular biology 33:3461-72 PubMed23816881

2013

The casein kinase 2-nrf1 axis controls the clearance of ubiquitinated proteins by regulating proteasome gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshiki Tsuchiya,Hiroaki Taniguchi,Yoshiyuki Ito,Tomoko Morita,M Rezaul Karim,Norihito Ohtake,Kousuke Fukagai,Takao Ito,Shota Okamuro,Shun-Ichiro Iemura,Tohru Natsume,Eisuke Nishida,Akira Kobayashi

Molecular and cellular biochemistry 353:81-91 PubMed21431367

2011

The cytosolic protein kinase CK2 phosphorylates cardiac calsequestrin in intact cells.

Applications

WB

Species

Human

Timothy P McFarland,Naama H Sleiman,Daniel B Yaeger,Steven E Cala

The Journal of biological chemistry 285:1661-70 PubMed19933278

2009

Differential regulation of mitogen- and stress-activated protein kinase-1 and -2 (MSK1 and MSK2) by CK2 following UV radiation.

Applications

WB

Species

Unspecified reactive species

Kellie A Jacks,C Anne Koch
View all publications
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