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重组RabMAb

重组Anti-CSNK1A1 + CSNK1A1L抗体[EPR1961(2)] (ab108296)

Reviews (2) Submit a question References (19)
Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296)
  • Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296)
  • Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296)
  • Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296)
  • Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296)
  • Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1961(2)] to CSNK1A1 + CSNK1A1L
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-CSNK1A1 + CSNK1A1L抗体[EPR1961(2)]
    参阅全部 CSNK1A1 + CSNK1A1L 一抗

  • 描述

    兔单克隆抗体[EPR1961(2)] to CSNK1A1 + CSNK1A1L

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: MCF-7, HeLa, Jurkat, Rat brain lysates, RAW 264.7, PC-12 and HT-1376 cell lysates. N-GST tagged Human CSNK1A1 and CSNK1A1L full-length recombinant protein.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab108296于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (2)
1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 39 kDa).

For unpurified use at 1/1000 - 1/10000
说明
WB
1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 39 kDa).

For unpurified use at 1/1000 - 1/10000

靶标

图片

  • Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296)
    Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296)
    All lanes : Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296) at 1/1000 dilution

    Lane 1 : N-GST tagged Human CSNK1A1L full-length (aa1 to 337) recombinant protein 10ng
    Lane 2 : N-GST tagged Human CSNK1A1 full-length (aa1 to 337) recombinant protein 10ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 39 kDa

  • Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296)
    Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296)
    All lanes : Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296) at 1/1000 dilution (purified)

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
    Lane 2 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 39 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296)
    Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296)
    Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296) at 1/2000 dilution + RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 39 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296)
    Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296)
    All lanes : Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296) at 1/1000 dilution (purified)

    Lane 1 : Rat brain lysates
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 39 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296)
    Western blot - Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296)
    All lanes : Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296) at 1/1000 dilution (unpurified)

    Lane 1 : MCF7 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HT-1376 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 39 kDa
    Observed band size: 34 kDa why is the actual band size different from the predicted?

  • Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296)
    Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296)

文献 (19)

发表研究结果有使用 ab108296？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab108296 被引用在 19 文献中.

  • Yamanaka S  et al. Lenalidomide derivatives and proteolysis-targeting chimeras for controlling neosubstrate degradation. Nat Commun 14:4683 (2023). PubMed: 37596276
  • Teng M  et al. Development of PDE6D and CK1α Degraders through Chemical Derivatization of FPFT-2216. J Med Chem 65:747-756 (2022). PubMed: 34965125
  • Kang K  et al. Dishevelled phase separation promotes Wnt signalosome assembly and destruction complex disassembly. J Cell Biol 221:N/A (2022). PubMed: 36342472
  • Aubrey BJ  et al. IKAROS and MENIN coordinate therapeutically actionable leukemogenic gene expression in MLL-r acute myeloid leukemia. Nat Cancer 3:595-613 (2022). PubMed: 35534777
  • Yamanaka S  et al. A proximity biotinylation-based approach to identify protein-E3 ligase interactions induced by PROTACs and molecular glues. Nat Commun 13:183 (2022). PubMed: 35013300
View all Publications for this product

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Casein Kinase 1 alpha antibody [EPR1961(2)]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse macrophage)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
15 µg
Specification
Mouse macrophage
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 20 2017

Western blot abreview for Anti-Casein Kinase 1 alpha antibody [EPR1961(2)]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (lymphoid cell)
Gel Running Conditions
Reduced Denaturing (10 % gel)
Loading amount
10 µg
Specification
lymphoid cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 18 2015

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