重组Anti-CSN7b抗体[EPR6464] (ab133548)
![Western blot - Anti-CSN7b antibody [EPR6464] (ab133548)](https://www.abcam.cn/ps/products/133/ab133548/Images/ab133548-393616-anti-csn7b-antibody-epr6464-western-blot-human-knockout-lysate.jpg "Western blot - Anti-CSN7b antibody [EPR6464] (ab133548)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6464] to CSN7b
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
-
产品名称
Anti-CSN7b抗体[EPR6464]
参阅全部 CSN7b 一抗 -
描述
兔单克隆抗体[EPR6464] to CSN7b -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-Pmore details
不适用于: IP -
种属反应性
与反应: Mouse, Human
预测可用于: Rat
-
免疫原
Synthetic peptide within Human CSN7b aa 50-150. The exact sequence is proprietary.
-
阳性对照
- WB: HEK293T, HAP1, Jurkat and HeLa cell lysates. IHC-P: Human kidney tissue.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 1.12 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6464 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
KO cell lines
-
KO cell lysates
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133548于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/200.
|
|
| WB | (1) |
1/10000 - 1/50000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
|
| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/200. |
|
WB
1/10000 - 1/50000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
-
功能
Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, JUN, I-kappa-B-alpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. -
序列相似性
Belongs to the CSN7/EIF3M family. CSN7 subfamily.
Contains 1 PCI domain. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 64708 Human
- Entrez Gene: 26895 Mouse
- Entrez Gene: 363273 Rat
- Omim: 616010 Human
- SwissProt: Q9H9Q2 Human
- SwissProt: Q8BV13 Mouse
- Unigene: 335061 Human
- Unigene: 12973 Mouse
-
别名
- COP9 Complex Homolog Subunit 7b antibody
- COP9 Constitutive Photomorphogenic Homolog Subunit 7b antibody
- COP9 Signalasome Subunit 7b antibody
see all
图片
-
Western blot - Anti-CSN7b antibody [EPR6464] (ab133548)All lanes : Anti-CSN7b antibody [EPR6464] (ab133548) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : COPS7B knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 30 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab133548 observed at 32 kDa. Red - loading control ab8245 observed at 36 kDa.
ab133548 Anti-CSN7b antibody [EPR6464] was shown to specifically react with CSN7b in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266646 (knockout cell lysate ab257895) was used. Wild-type and CSN7b knockout samples were subjected to SDS-PAGE. ab133548 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-CSN7b antibody [EPR6464] (ab133548)](https://www.abcam.cn/ps/products/133/ab133548/Images/ab133548-266733-ab133548KOWB.jpg)
Western blot - Anti-CSN7b antibody [EPR6464] (ab133548)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: CSN7b knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab133548 observed at 32 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab133548 was shown to specifically react with CSN7b when CSN7b knockout samples were used. Wild-type and CSN7b knockout samples were subjected to SDS-PAGE. ab133548 and ab18058 (loading control to vinculin) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Flow Cytometry (Intracellular) - Anti-CSN7b antibody [EPR6464] (ab133548)](https://www.abcam.cn/ps/products/133/ab133548/Images/ab133548-275869-ab133548FC1.jpg)
Flow Cytometry (Intracellular) - Anti-CSN7b antibody [EPR6464] (ab133548)Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling CSN7b with unpurified ab133548 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti-rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN7b antibody [EPR6464] (ab133548)](https://www.abcam.cn/ps/products/133/ab133548/Images/CSN7b-Primary-antibodies-ab133548-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN7b antibody [EPR6464] (ab133548)Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling CSN7b with ab133548 antibody at a dilution of 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Western blot - Anti-CSN7b antibody [EPR6464] (ab133548)](https://www.abcam.cn/ps/products/133/ab133548/Images/CSN7b-Primary-antibodies-ab133548-1.jpg)
Western blot - Anti-CSN7b antibody [EPR6464] (ab133548)All lanes : Anti-CSN7b antibody [EPR6464] (ab133548) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa -
![OI-RD Scanning - Anti-CSN7b antibody [EPR6464] (ab133548)](https://www.abcam.cn/ps/products/133/ab133548/Images/CSN7b-Primary-antibodies-ab133548-3.jpg)
OI-RD Scanning - Anti-CSN7b antibody [EPR6464] (ab133548) -
![Anti-CSN7b antibody [EPR6464] (ab133548)](https://www.abcam.cn/ps/products/133/ab133548/Images/ab133548-7-benefits-of-recombinant-antibodies.png)
Anti-CSN7b antibody [EPR6464] (ab133548)
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab133548 被引用在 2 文献中.
- Liang E et al. COP9 Signalosome Subunit 3 Restricts Neuroinflammatory Responses During Cerebral Ischemia/Reperfusion Injury Through Stabilizing Suppressor of Cytokine Signaling 3 Protein. Neuropsychiatr Dis Treat 17:1217-1227 (2021). PubMed: 33911869
- Liu J et al. A genome-scale CRISPR-Cas9 screening in myeloma cells identifies regulators of immunomodulatory drug sensitivity. Leukemia N/A:N/A (2018). PubMed: 30026574