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Signal Transduction Protein Phosphorylation Tyrosine Kinases Src Family

Anti-CSK抗体(ab744)

  • Datasheet
Submit a review Q&A (1)References (2)

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Western blot - Anti-CSK antibody (ab744)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSK antibody (ab744)
  • Western blot - Anti-CSK antibody (ab744)

Key features and details

  • Goat polyclonal to CSK
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-CSK抗体
    参阅全部 CSK 一抗
  • 描述

    山羊多克隆抗体to CSK
  • 宿主

    Goat
  • 经测试应用

    适用于: WB, IHC-Pmore details
  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to Human CSK aa 400 to the C-terminus (C terminal).
    Database link: NP_004374.1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • Jurkat cell lysate, Human colon tissue.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Src Family
    • Neuroscience
    • Development

相关产品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant human CSK protein (ab60764)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab744于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).

1 hour primary incubation is recommended for this product.

IHC-P
Use at an assay dependent concentration.
说明
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).

1 hour primary incubation is recommended for this product.

IHC-P
Use at an assay dependent concentration.

靶标

  • 功能

    Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.
  • 组织特异性

    Expressed in lung and macrophages.
  • 序列相似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • 翻译后修饰

    Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.
  • 细胞定位

    Cytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts.
  • Target information above from: UniProt accession P41240 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 509246 Cow
    • Entrez Gene: 487649 Dog
    • Entrez Gene: 1445 Human
    • Entrez Gene: 12988 Mouse
    • Omim: 124095 Human
    • SwissProt: Q0VBZ0 Cow
    • SwissProt: P41240 Human
    • SwissProt: P41241 Mouse
    • Unigene: 77793 Human
    • Unigene: 21974 Mouse
    • Unigene: 470150 Mouse
    see all
  • 别名

    • C SRC antibody
    • C SRC kinase antibody
    • C src Tyrosine Kinase antibody
    • C-SRC kinase antibody
    • c-src tyrosine kinase antibody
    • Csk A antibody
    • CSK antibody
    • CSK_HUMAN antibody
    • CYTOPLASMIC TYROSINE KINASE antibody
    • EC 2.7.10.2 antibody
    • MGC112926 antibody
    • MGC117393 antibody
    • MGC154049 antibody
    • P60 Src antibody
    • Protein tyrosine kinase CYL antibody
    • Protein-tyrosine kinase CYL antibody
    • Proto oncogene tyrosine protein kinase antibody
    • Tyrosine protein kinase CSK antibody
    • Tyrosine-protein kinase CSK antibody
    • zgc:154049 antibody
    see all

图片

  • Western blot - Anti-CSK antibody (ab744)
    Western blot - Anti-CSK antibody (ab744)
    ab744 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. ab744 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSK antibody (ab744)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSK antibody (ab744)
    ab744 at 4µg/ml staining CSK in Human Colon by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
  • Western blot - Anti-CSK antibody (ab744)
    Western blot - Anti-CSK antibody (ab744)
    Anti-CSK antibody (ab744) at 1 µg/ml + Mouse spleen lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 52 kDa

实验方案

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

数据表及文件

  • Datasheet download

    Download

文献 (2)

发表研究结果有使用 ab744?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab744 被引用在 2 文献中.

  • Yeh WL  et al. Fulvestrant-induced cell death and proteasomal degradation of estrogen receptor a protein in MCF-7 cells require the CSK c-Src tyrosine kinase. PLoS One 8:e60889 (2013). WB ; Human . PubMed: 23593342
  • Ribeiro-Oliveira A  et al. Protein western array analysis in human pituitary tumours: insights and limitations. Endocr Relat Cancer 15:1099-114 (2008). WB ; Human . PubMed: 18701577

客户评价及客户问答

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Question

Has this antibody ever been tested on any samples other than Jurkat lysate?

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Abcam community

Verified customer

Asked on Jun 01 2005

Answer

Thank you for your enquiry. I contacted the originator of this antibody and they said they also did a preliminary Western blot with mouse spleen lysate and saw a band at approximately 45 kDa - but they aren't sure if it's correct. Please let me know if you need additional assistance.

Read More

Abcam Scientific Support

回复于 Jun 02 2005

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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