Anti-CSF-1-R抗体(ab223642)
Key features and details
- Goat polyclonal to CSF-1-R
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-CSF-1-R抗体
参阅全部 CSF-1-R 一抗 -
描述
山羊多克隆抗体to CSF-1-R -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cat
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免疫原
Synthetic peptide corresponding to Human CSF-1-R aa 700-800 (internal sequence) (Cysteine residue). (NP_005202.2).
Database link: P07333 -
阳性对照
- WB: HEK-293 lysate overexpressing human CSF-1-R with C-terminal MYC tag.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab223642 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223642于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 108 kDa.
A 1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 108 kDa. A 1 hour primary incubation is recommended for this product. |
靶标
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功能
Protein tyrosine-kinase transmembrane receptor for CSF1 and IL34. -
组织特异性
Expressed in bone marrow and in differentiated blood mononuclear cells. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 493706 Cat
- Entrez Gene: 1436 Human
- Entrez Gene: 12978 Mouse
- Entrez Gene: 307403 Rat
- Omim: 164770 Human
- SwissProt: P13369 Cat
- SwissProt: P07333 Human
- SwissProt: P09581 Mouse
see all -
别名
- C FMS antibody
- CD 115 antibody
- CD115 antibody
see all
图片
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Western blot - Anti-CSF-1-R antibody (ab223642)Lane 1 : Anti-CSF-1-R antibody (ab223642) at 0.5 µg/ml
Lane 2 : Anti-CSF-1-R antibody (ab223642) at 1 mg/ml
Lane 3 : Anti-MYC tag at 1/1000 dilution
Lanes 1 & 3 : HEK-293 (human epithelial cell line from embryonic kidney) lysate overexpressing human CSF-1-R with C-terminal MYC tag (in RIPA buffer)
Lane 2 : Mock transfected HEK-293 (human epithelial cell line from embryonic kidney) lysate (in RIPA buffer)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 108 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?Primary incubations were for 1 hour.
数据表及文件
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Datasheet download
文献 (0)
ab223642 尚未被引用在任何文献中。
