重组Anti-CSDE1/NRU抗体[EPR17414]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSDE1/NRU antibody [EPR17414] (AB201688)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling CSDE1/NRU with ab201688 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on Human kidney tissue is observed.

Counter stained with Hematoxylin.

Negative control : Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CSDE1/NRU antibody [EPR17414] (AB201688)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling CSDE1/NRU with ab201688 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

Confocal image showing cytoplasmic staining on HeLa cell line is observed.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. ab201688 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSDE1/NRU antibody [EPR17414] (AB201688)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling CSDE1/NRU with ab201688 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on Human breast carcinoma tissue is observed.

Counter stained with Hematoxylin.

Negative control : Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CSDE1/NRU antibody [EPR17414] (AB201688)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling CSDE1/NRU with ab201688 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

Confocal image showing cytoplasmic staining on MCF7 cell line is observed.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. ab201688 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

• IP

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Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17414] (AB201688)

CSDE1/NRU was immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab201688 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab201688 at 1/1500 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500 dilution.

Lane 1 : K562 whole cell extract 10 μg (Input).

Lane 2 : ab201688 IP in K562 whole cell extract.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab20688 in K562 whole cell extract.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second

All lanes:

Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17414] (ab201688)

Predicted band size: 89 kDa

false

• WB

Supplier Data

Western blot - Anti-CSDE1/NRU antibody [EPR17414] (AB201688)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CSDE1/NRU antibody [EPR17414] (ab201688) at 1/1000 dilution

All lanes:

Human testis at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 89 kDa

Observed band size: 89 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-CSDE1/NRU antibody [EPR17414] (AB201688)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CSDE1/NRU antibody [EPR17414] (ab201688) at 1/10000 dilution

Lane 1:

K562 (Human chronic myelogenous leukemia cells from bone marrow) lysate at 20 µg

Lane 2:

HEK293 (Human embryonic kidney) at 20 µg

Lane 3:

HeLa (Human epithelial cells from cervix adenocarcinoma) lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 89 kDa

Observed band size: 89 kDa

false

Exposure time: 1min

• WB

Supplier Data

Western blot - Anti-CSDE1/NRU antibody [EPR17414] (AB201688)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CSDE1/NRU antibody [EPR17414] (ab201688) at 1/1000 dilution

Lane 1:

Mouse brain at 10 µg

Lane 2:

Rat brain at 10 µg

Lane 3:

Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 89 kDa

Observed band size: 89 kDa

false

Exposure time: 1min