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AB91654

重组Anti-CRTC3抗体[EPR3440]

Anti-CRTC3 antibody [EPR3440]

5

(2 Reviews)

|

(12 Publications)

Anti-CRTC3 antibody [EPR3440] (ab91654) is a rabbit monoclonal antibody detecting CRTC3 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
- Trusted since 2010

查看别名

TORC3, CRTC3, CREB-regulated transcription coactivator 3, Transducer of regulated cAMP response element-binding protein 3, TORC-3, Transducer of CREB protein 3

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (AB91654)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (AB91654)

Immunohistochemical staining of paraffin embedded human tonsil with purified ab91654 at a working dilution of 1/250. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (AB91654)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (AB91654)

Immunohistochemical staining of paraffin embedded mouse spleen with purified ab91654 at a working dilution of 1/250. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Western blot - Anti-CRTC3 antibody [EPR3440] (AB91654)
  • WB

Unknown

Western blot - Anti-CRTC3 antibody [EPR3440] (AB91654)

All lanes:

Western blot - Anti-CRTC3 antibody [EPR3440] (ab91654) at 1/1000 dilution

Lane 1:

293T cell lysates at 10 µg

Lane 2:

Jurkat cell lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 67 kDa

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Western blot - Anti-CRTC3 antibody [EPR3440] (AB91654)
  • WB

Lab

Western blot - Anti-CRTC3 antibody [EPR3440] (AB91654)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-CRTC3 antibody [EPR3440] (ab91654) at 1/20000 dilution

Lane 1:

HEK293 whole cell lysate at 20 µg

Lane 2:

HeLa whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 67 kDa

Observed band size: 76 kDa

false

Western blot - Anti-CRTC3 antibody [EPR3440] (AB91654)
  • WB

Lab

Western blot - Anti-CRTC3 antibody [EPR3440] (AB91654)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-CRTC3 antibody [EPR3440] (ab91654) at 1/20000 dilution

Lane 1:

Raw 264.7 whole cell lysate at 20 µg

Lane 2:

PC-12 whole cell lysate at 20 µg

Lane 3:

NIH/3T3 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 67 kDa

Observed band size: 76 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (AB91654)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (AB91654)

Immunohistochemical staining of paraffin embedded rat spleen with purified ab91654 at a working dilution of 1/250. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (AB91654)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (AB91654)

Unpurified ab91654 at 1/100 dilution staining CRTC3 in Human tonsil by immunohistochemistry (paraffin-embedded section).

不同偶联物与剂型 (2)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR3440

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

ICC/IF, IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "<p><strong>For unpurified, use 1/1000 - 10000.</strong></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "<p><strong>For unpurified, use 1/1000 - 10000.</strong></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>" } } }
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为什么推荐这个？

我们推荐这个产品，因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

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产品详情

What is this antibody validated in?
Anti-CRTC3 antibody [EPR3440] (ab91654) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of CRTC3?
Anti-CRTC3 [EPR3440] (ab91654) specifically detects a band for CRTC3 (UniProt: Q6UUV7) at a molecular weight of 67kDa.

Trusted by the scientific community
Anti-CRTC3 [EPR3440] (ab91654) was first used in a scientific publication in 2010 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR3440] also available for your convenience: ab91654, Alexa Fluor® 488 - ab206248, Carrier free - ab220809, Biotin - ab302836

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).
See full target information CRTC3
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文献 (12)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 9:331 PubMed37666810

2023

In vitro CRISPR screening uncovers CRTC3 as a regulator of IFN-γ-induced ferroptosis of hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Li Li,Tao Xing,Yiran Chen,Weiran Xu,Bo Fan,Gaoda Ju,Jing Zhao,Li Lin,Cihui Yan,Jun Liang,Xiubao Ren

Journal of nanobiotechnology 20:453 PubMed36243711

2022

TiSe-mediated sonodynamic and checkpoint blockade combined immunotherapy in hypoxic pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Libin Chen,Wang Xue,Jing Cao,Shengmin Zhang,Yiqing Zeng,Ling Ma,Xuechen Qian,Qing Wen,Yurong Hong,Zhan Shi,Youfeng Xu

Arteriosclerosis, thrombosis, and vascular biology 41:2431-2451 PubMed34196217

2021

Salt-Inducible Kinase 3 Promotes Vascular Smooth Muscle Cell Proliferation and Arterial Restenosis by Regulating AKT and PKA-CREB Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yujun Cai,Xue-Lin Wang,Jinny Lu,Xin Lin,Jonathan Dong,Raul J Guzman

Reproduction (Cambridge, England) 161:573-580 PubMed33780908

2021

TORC2/3-mediated DUSP1 upregulation is essential for human decidualization.

Applications

Unspecified application

Species

Unspecified reactive species

Chunfang Xu,Weijie Zhao,Xixi Huang,Zhuxuan Jiang,Lu Liu,Liyuan Cui,Xinyi Li,Dajin Li,Meirong Du

Journal of neuroendocrinology 33:e12920 PubMed33314405

2020

Activation and expression of endogenous CREB-regulated transcription coactivators (CRTC) 1, 2 and 3 in the rat adrenal gland.

Applications

Unspecified application

Species

Unspecified reactive species

Lorna I F Smith,Zidong Zhao,Jamie Walker,Stafford Lightman,Francesca Spiga

Molecular and cellular endocrinology 499:110612 PubMed31604124

2019

Involvement of CREB-regulated transcription coactivators (CRTC) in transcriptional activation of steroidogenic acute regulatory protein (Star) by ACTH.

Applications

Unspecified application

Species

Unspecified reactive species

Lorna I F Smith,Victoria Huang,Mark Olah,Loc Trinh,Ying Liu,Georgina Hazell,Becky Conway-Campbell,Zidong Zhao,Antoine Martinez,Anne-Marie Lefrançois-Martinez,Stafford Lightman,Francesca Spiga,Greti Aguilera

Cancer discovery 9:1606-1627 PubMed31350328

2019

The AMPK-Related Kinases SIK1 and SIK3 Mediate Key Tumor-Suppressive Effects of LKB1 in NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Pablo E Hollstein,Lillian J Eichner,Sonja N Brun,Anwesh Kamireddy,Robert U Svensson,Liliana I Vera,Debbie S Ross,T J Rymoff,Amanda Hutchins,Hector M Galvez,April E Williams,Maxim N Shokhirev,Robert A Screaton,Rebecca Berdeaux,Reuben J Shaw

Journal of leukocyte biology 99:711-21 PubMed26590148

2015

SIK inhibition in human myeloid cells modulates TLR and IL-1R signaling and induces an anti-inflammatory phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Stella Lombardi,Corine Gilliéron,Damien Dietrich,Cem Gabay

The Journal of neuroscience : the official journal of the Society for Neuroscience 35:12248-60 PubMed26338335

2015

Oxytocin Regulates Stress-Induced Crf Gene Transcription through CREB-Regulated Transcription Coactivator 3.

Applications

ChIP, WB

Species

Human, Human

Benjamin Jurek,David A Slattery,Yuichi Hiraoka,Ying Liu,Katsuhiko Nishimori,Greti Aguilera,Inga D Neumann,Erwin H van den Burg

Nature communications 5:4535 PubMed25088745

2014

The LKB1-salt-inducible kinase pathway functions as a key gluconeogenic suppressor in the liver.

Applications

WB

Species

Unspecified reactive species

Kashyap Patel,Marc Foretz,Allison Marion,David G Campbell,Robert Gourlay,Nadia Boudaba,Emilie Tournier,Paul Titchenell,Mark Peggie,Maria Deak,Min Wan,Klaus H Kaestner,Olga Göransson,Benoit Viollet,Nathanael S Gray,Morris J Birnbaum,Calum Sutherland,Kei Sakamoto
View all publications
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