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重组RabMAb

重组Anti-CRTAM抗体[EPR23786-12] - BSA and Azide free (ab273884)

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Flow Cytometry - Anti-CRTAM antibody [EPR23786-12] - BSA and Azide free (ab273884)
  • Anti-CRTAM antibody [EPR23786-12] - BSA and Azide free (ab273884)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23786-12] to CRTAM - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-CRTAM抗体[EPR23786-12] - BSA and Azide free
    参阅全部 CRTAM 一抗

  • 描述

    兔单克隆抗体[EPR23786-12] to CRTAM - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, Flow Cytmore details
    不适用于: ICC/IF,IHC-P or IP

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Mouse thymus tissue lysate; Rat cerebellum tissue lysate. Flow cyt: Human PBMCs.

  • 常规说明

    ab273884 is the carrier-free version of ab272723.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab273884于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 45 kDa).
Flow Cyt
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 45 kDa).
Flow Cyt
Use at an assay dependent concentration.
应用说明
Is unsuitable for ICC/IF,IHC-P or IP.

靶标

  • 功能

    Interaction with CADM1 promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo.

  • 组织特异性

    In the immune system, expression is restricted to activated class-I MHC-restricted cells, including NKT and CD8 cells. Strongly expressed in spleen, thymus, small intestine, peripheral blood leukocyte, and in Purkinje neurons in cerebellum. Expressed at much lower levels in testis, ovary, colon, lung and lymphoid tissues.

  • 序列相似性

    Belongs to the nectin family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • CD355 antibody
    • Class I MHC restricted T cell associated molecule antibody
    • Class-I MHC-restricted T-cell-associated molecule antibody
    • CRTAM antibody
    • CRTAM_HUMAN antibody
    • Cytotoxic and regulatory T cell molecule antibody
    • Cytotoxic and regulatory T-cell molecule antibody
    see all

图片

  • Flow Cytometry - Anti-CRTAM antibody [EPR23786-12] - BSA and Azide free (ab273884)
    Flow Cytometry - Anti-CRTAM antibody [EPR23786-12] - BSA and Azide free (ab273884)

    Flow cytometric analysis of Human peripheral blood mononuclear cells (PBMC) (treated with 1 ng/mL PMA and 0.2 μM Ionomycin for 5h) labelling CRTAM with ab272723 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG or ab272723. Then stained with anti-CD8 alpha conjugated to Pacific blue.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272723).

  • Anti-CRTAM antibody [EPR23786-12] - BSA and Azide free (ab273884)
    Anti-CRTAM antibody [EPR23786-12] - BSA and Azide free (ab273884)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

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ab273884 尚未被引用在任何文献中。

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