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重组RabMAb

重组Anti-CRTAM抗体[EPR23786-12] (ab272723)

Submit a review Submit a question References (1)
Flow Cytometry - Anti-CRTAM antibody [EPR23786-12] (ab272723)
  • Western blot - Anti-CRTAM antibody [EPR23786-12] (ab272723)
  • Western blot - Anti-CRTAM antibody [EPR23786-12] (ab272723)
  • Anti-CRTAM antibody [EPR23786-12] (ab272723)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23786-12] to CRTAM
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-CRTAM抗体[EPR23786-12]
    参阅全部 CRTAM 一抗

  • 描述

    兔单克隆抗体[EPR23786-12] to CRTAM

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, Flow Cytmore details
    不适用于: ICC/IF,IHC-P or IP

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Mouse thymus tissue lysate; Rat cerebellum tissue lysate; Human PBMC (untreated and treated with 1ng/mL phorbol-12-myristate-13-acetate and 0.2 µM ionomycin for 5 hours) whole cell lysates. Flow Cyt: Human PBMCs.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR23786-12

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab272723于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 45 kDa).
Flow Cyt
1/2110.
说明
WB
1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 45 kDa).
Flow Cyt
1/2110.
应用说明
Is unsuitable for ICC/IF,IHC-P or IP.

靶标

  • 功能

    Interaction with CADM1 promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo.

  • 组织特异性

    In the immune system, expression is restricted to activated class-I MHC-restricted cells, including NKT and CD8 cells. Strongly expressed in spleen, thymus, small intestine, peripheral blood leukocyte, and in Purkinje neurons in cerebellum. Expressed at much lower levels in testis, ovary, colon, lung and lymphoid tissues.

  • 序列相似性

    Belongs to the nectin family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • CD355 antibody
    • Class I MHC restricted T cell associated molecule antibody
    • Class-I MHC-restricted T-cell-associated molecule antibody
    • CRTAM antibody
    • CRTAM_HUMAN antibody
    • Cytotoxic and regulatory T cell molecule antibody
    • Cytotoxic and regulatory T-cell molecule antibody
    see all

图片

  • Flow Cytometry - Anti-CRTAM antibody [EPR23786-12] (ab272723)
    Flow Cytometry - Anti-CRTAM antibody [EPR23786-12] (ab272723)

    Flow cytometric analysis of Human peripheral blood mononuclear cells (PBMC) (treated with 1 ng/mL PMA and 0.2 μM Ionomycin for 5h) labelling CRTAM with ab272723 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG or ab272723. Then stained with anti-CD8 alpha conjugated to Pacific blue.

    Gated on viable cells.

  • Western blot - Anti-CRTAM antibody [EPR23786-12] (ab272723)
    Western blot - Anti-CRTAM antibody [EPR23786-12] (ab272723)
    All lanes : Anti-CRTAM antibody [EPR23786-12] (ab272723) at 1/1000 dilution

    Lane 1 : Untreated human peripheral blood mononuclear cell (PBMC) whole cell lysate
    Lane 2 : Human PBMC treated with 1ng/mL phorbol-12-myristate-13-acetate (PMA) (ab120297) and 0.2 µM ionomycin (ab120116) for 5 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody)

    Predicted band size: 45 kDa
    Observed band size: 70,85 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    The molecular weight observed is consistent with what has been described in the literature (PMID:10811014, 23637756, 16300832).

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Western blot - Anti-CRTAM antibody [EPR23786-12] (ab272723)
    Western blot - Anti-CRTAM antibody [EPR23786-12] (ab272723)
    All lanes : Anti-CRTAM antibody [EPR23786-12] (ab272723) at 1/1000 dilution

    Lane 1 : Mouse thymus tissue lysate
    Lane 2 : Rat cerebellum tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 45 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    The molecular weight observed is consistent with what has been described in the literature (PMID:10811014, 23637756, 16300832).

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Anti-CRTAM antibody [EPR23786-12] (ab272723)
    Anti-CRTAM antibody [EPR23786-12] (ab272723)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (1)

发表研究结果有使用 ab272723？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab272723 被引用在 1 文献中.

  • He X  et al. Identification and Validation of Hub Genes for Predicting Treatment Targets and Immune Landscape in Rheumatoid Arthritis. Biomed Res Int 2022:8023779 (2022). PubMed: 36317112

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