Anti-CRSP2 / MED14抗体(ab72141)
Key features and details
- Rabbit polyclonal to CRSP2 / MED14
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CRSP2 / MED14抗体
参阅全部 CRSP2 / MED14 一抗 -
描述
兔多克隆抗体to CRSP2 / MED14 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Whole cell lysate from Hela cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72141于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | ||
| IP |
| 说明 |
|---|
应用说明
IP: Use at 2-5µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects bands of approximately 71 and 160 kDa and various other bands (predicted molecular weight: 160 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Detects bands of approximately 71 and 160 kDa and various other bands (predicted molecular weight: 160 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the Mediator complex subunit 14 family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 9282 Human
- Omim: 300182 Human
- SwissProt: O60244 Human
- Unigene: 407604 Human
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别名
- Activator-recruited cofactor 150 kDa component antibody
- ARC150 antibody
- Cofactor required for Sp1 transcriptional activation subunit 2 antibody
see all
图片
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Western blot - Anti-CRSP2 / MED14 antibody (ab72141)All lanes : Anti-CRSP2 / MED14 antibody (ab72141) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Predicted band size: 160 kDa
Observed band size: 160 kDa
Additional bands at: 120 kDa, 71 kDa. We are unsure as to the identity of these extra bands.
Various other bands are detected. -
Immunoprecipitation - Anti-CRSP2 / MED14 antibody (ab72141)Immunoprecipitation/ Western Blot of CRSP2 / MED14.
Lane 1: ab72141 at 3µg/mg whole cell lysate for IP.
Lane 2: Control IgG.
ab72141 at 1µg/ml for subsequent WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection:Chemiluminescence with an exposure time of 3 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab72141 被引用在 3 文献中.
- Pintacuda G et al. Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders. Cell Genom 3:100250 (2023). PubMed: 36950384
- Batlle C et al. MED15 prion-like domain forms a coiled-coil responsible for its amyloid conversion and propagation. Commun Biol 4:414 (2021). PubMed: 33772081
- Ruiz A et al. Characterization of the influence of mediator complex in HIV-1 transcription. J Biol Chem 289:27665-76 (2014). Human . PubMed: 25100719