Anti-CROP抗体(ab80412)
Key features and details
- Rabbit polyclonal to CROP
- Suitable for: IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-CROP抗体
参阅全部 CROP 一抗 -
描述
兔多克隆抗体to CROP -
宿主
Rabbit -
经测试应用
适用于: IHC-P, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Chicken, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Tilapia, Orangutan
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免疫原
Synthetic peptide corresponding to a region between residue 1 and 50 of human CROP (NP_057508.2).
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阳性对照
- IHC-P: Human testicular seminoma, mouse colon carcinoma tissue. WB: HeLa cells whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab80412于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Binds cAMP regulatory element DNA sequence. May play a role in RNA splicing. -
组织特异性
Widely expressed. Highest levels in heart, brain, pancreas, thymus, ovary, small intestine and peripheral blood leukocytes, as well as cerebellum, putamen and pituitary gland. Lowest levels in lung, liver and kidney. Also expressed in fetal tissues, including brain, heart, kidney, thymus and lung. -
序列相似性
Belongs to the Luc7 family. -
翻译后修饰
Phosphorylated in vitro by SRPK1, SRPK2 and CLK1. -
细胞定位
Nucleus speckle. The subnuclear localization is affected by cisplatin. - Information by UniProt
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数据库链接
- Entrez Gene: 539955 Cow
- Entrez Gene: 51747 Human
- Entrez Gene: 67684 Mouse
- Entrez Gene: 100172991 Orangutan
- Entrez Gene: 360602 Rat
- Omim: 609434 Human
- SwissProt: Q3SX41 Cow
- SwissProt: O95232 Human
see all -
别名
- cAMP regulatory element-associated protein 1 antibody
- Cisplatin resistance-associated overexpressed protein antibody
- Cisplatin resistance-associated-overexpressed protein antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CROP antibody (ab80412)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma (left) and mouse colon carcinoma (right) tissue labelling CROP with ab80412 at 1/1000 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-CROP antibody (ab80412)Detection of Human CROP in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab80412 at 3 µg/mg lysate for IP (lane 1). ab80412 was used at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab80412 尚未被引用在任何文献中。