Anti-CRMP2 (phospho T514)抗体(ab62478)
Key features and details
- Rabbit polyclonal to CRMP2 (phospho T514)
- Suitable for: ELISA, ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-CRMP2 (phospho T514)抗体
参阅全部 CRMP2 一抗 -
描述
兔多克隆抗体to CRMP2 (phospho T514) -
宿主
Rabbit -
经测试应用
适用于: ELISA, ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human CRMP2 aa 450-550 (phospho T514).
Database link: Q16555 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab62478 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62478于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
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| ICC/IF |
1/500 - 1/1000.
|
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| IHC-P |
1/50 - 1/100.
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| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
|
| 说明 |
|---|
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ELISA
Use at an assay dependent concentration. |
|
ICC/IF
1/500 - 1/1000. |
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IHC-P
1/50 - 1/100. |
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa). |
靶标
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功能
Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily. -
翻译后修饰
3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 1808 Human
- Entrez Gene: 12934 Mouse
- Entrez Gene: 25416 Rat
- Omim: 602463 Human
- SwissProt: Q16555 Human
- SwissProt: O08553 Mouse
- SwissProt: P47942 Rat
- Unigene: 173381 Human
see all -
别名
- Collapsin response mediator protein 2 antibody
- Collapsin response mediator protein antibody
- Collapsin response mediator protein hCRMP 2 antibody
see all
图片
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Western blot - Anti-CRMP2 (phospho T514) antibody (ab62478)Anti-CRMP2 (phospho T514) antibody (ab62478) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 63 kDa
Observed band size: 63 kDa
Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP2 (phospho T514) antibody (ab62478)ab62478, at 1/50 dilution, staining CRMP2 in paraffin embedded human brain tissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunising peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-CRMP2 (phospho T514) antibody (ab62478)ab62478, at 1/500 dilution, staining CRMP2 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-CRMP2 (phospho T514) antibody (ab62478)Image from Ou Y et al., PLoS ONE 7(11): e48773. Fig 5.; doi: 10.1371/journal.pone.0048773. Epub 2012 Nov 21.Immunofluorescence analysis of RPE (human retinal pigmented epithelium) cells, staining CRMP2 (phospho T514) with ab62478. Cells were fixed in cold methanol and incubated with primary antibody, followed by an AlexaFluor®488-conjugated anti rabbit IgG.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab62478 被引用在 14 文献中.
- Paik S et al. Somatostatin Ameliorates ß-Amyloid-Induced Cytotoxicity via the Regulation of CRMP2 Phosphorylation and Calcium Homeostasis in SH-SY5Y Cells. Biomedicines 9:N/A (2021). PubMed: 33401710
- Chen L et al. Nogo-A Induced Polymerization of Microtubule Is Involved in the Inflammatory Heat Hyperalgesia in Rat Dorsal Root Ganglion Neurons. Int J Mol Sci 22:N/A (2021). PubMed: 34638704
- Yang L et al. Effect of Neurorepair for Motor Functional Recovery Enhanced by Total Saponins From Trillium tschonoskii Maxim. Treatment in a Rat Model of Focal Ischemia. Front Pharmacol 12:763181 (2021). PubMed: 34955834
- Hansson O Biomarkers for neurodegenerative diseases. Nat Med 27:954-963 (2021). PubMed: 34083813
- Li W et al. Downregulation of hippocampal SIRT6 activates AKT/CRMP2 signaling and ameliorates chronic stress-induced depression-like behavior in mice. Acta Pharmacol Sin 41:1557-1567 (2020). PubMed: 32265492