重组Anti-CRMP2 + CRMP3 + CRMP4抗体[EPR7590] (ab128875)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7590] to CRMP4 + CRMP2 + CRMP3
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-CRMP2 + CRMP3 + CRMP4抗体[EPR7590] -
描述
兔单克隆抗体[EPR7590] to CRMP4 + CRMP2 + CRMP3 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Brain: cerebellum (right) (Human) Cytoplasmic Lysate - adult normal tissue (ab30062) can be used as a positive control in WB. SH-SY5Y, Neuro-2a, U-87 MG and Human cerebellum lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7590 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128875于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/10000 - 1/50000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
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说明 |
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WB
1/10000 - 1/50000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa). |
靶标
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细胞定位
CRMP4: Cytoplasm. Cell projection > growth cone. Colocalizes with synaptic vesicle protein 2 in the central region of the growth cone. CRMP2: Cytoplasm. CRMP3: Cytoplasm. -
数据库链接
- Entrez Gene: 10570 Human
- Entrez Gene: 1808 Human
- Entrez Gene: 1809 Human
- Entrez Gene: 12934 Mouse
- Entrez Gene: 22240 Mouse
- Entrez Gene: 26757 Mouse
- Entrez Gene: 25416 Rat
- Entrez Gene: 25417 Rat
see all
图片
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All lanes : Anti-CRMP2 + CRMP3 + CRMP4 antibody [EPR7590] (ab128875) at 1/10000 dilution
Lane 1 : His tagged full length CRMP1 recombinant protein, 15ng
Lane 2 : His tagged full length CRMP3 recombinant protein, 15ng
Lane 3 : His tagged full length CRMP2 recombinant protein, 15ng
Lane 4 : His tagged full length CRMP4 recombinant protein, 15ng
Lane 5 : His tagged full length CRMP5 recombinant protein, 15ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 50-75 kDa why is the actual band size different from the predicted?
Exposure time: 80 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
ab213204 was used as a loading control at 1/10000 dilution.
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All lanes : Anti-CRMP2 + CRMP3 + CRMP4 antibody [EPR7590] (ab128875) at 1/10000 dilution
Lane 1 : SH-SYSY cell lysate
Lane 2 : Neuro-2a cell lysate
Lane 3 : U-87 MG cell lysate
Lane 4 : Human cerebellum lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 62 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab128875 被引用在 1 文献中.
- Hung YH et al. Semaphorin 4C promotes motility and immunosuppressive activity of cancer cells via CRMP3 and PD-L1. Am J Cancer Res 12:713-728 (2022). PubMed: 35261797