重组Anti-CRMP1抗体[EPR14520(B)] - N-terminal (ab180517)
![Western blot - Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517)](https://www.abcam.cn/ps/products/180/ab180517/Images/ab180517-211207-ab180517wb2.jpg "Western blot - Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14520(B)] to CRMP1 - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CRMP1抗体[EPR14520(B)] - N-terminal
参阅全部 CRMP1 一抗 -
描述
兔单克隆抗体[EPR14520(B)] to CRMP1 - N-terminal -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human CRMP1 aa 50-150 (N terminal). The exact sequence is proprietary.
Database link: Q14194 -
阳性对照
- Human cerebellum lysate, Fetal brain lysate, SH-SY5Y cell lysate, Paraffin-embedded Human brain sections.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR14520(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180517于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 62 kDa.
|
|
| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 62 kDa. |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. -
组织特异性
Brain. -
序列相似性
Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily. -
细胞定位
Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Associated with centrosomes and the mitotic spindle during metaphase. - Information by UniProt
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数据库链接
- Entrez Gene: 1400 Human
- Entrez Gene: 12933 Mouse
- Entrez Gene: 25415 Rat
- Omim: 602462 Human
- SwissProt: Q14194 Human
- SwissProt: P97427 Mouse
- SwissProt: Q62950 Rat
- Unigene: 135270 Human
see all -
别名
- Collapsin response mediator protein 1 antibody
- CRMP 1 antibody
- CRMP-1 antibody
see all
图片
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Western blot - Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517)All lanes : Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517) at 1/20000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : Fetal brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 62 kDa -
![Western blot - Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517)](https://www.abcam.cn/ps/products/180/ab180517/Images/ab180517-211206-ab180517wb.jpg)
Western blot - Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517)Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517) at 1/5000 dilution + Human cerebellum lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 62 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517)](https://www.abcam.cn/ps/products/180/ab180517/Images/ab180517-211208-ab180517IHCp.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517)Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling CRMP1 with ab180517 at 1/500 dilution. Anti-rabbit HRP seconday antibody was used and counterstaining with Hematoxylin was performed.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517)](https://www.abcam.cn/ps/products/180/ab180517/Images/ab180517-4-benefits-of-recombinant-antibodies.png)
Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517)
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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