Anti-CRM1抗体(ab3459)
Key features and details
- Rabbit polyclonal to CRM1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-CRM1抗体
参阅全部 CRM1 一抗 -
描述
兔多克隆抗体to CRM1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human CRM1 aa 1043-1058.
Sequence:RQADEEKHKRQMSVPGS
(Peptide available asab41768) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC’s. NPC’s contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Active transport of molecules through the NPC is a signal-mediated process that is not well understood. Proteins to be exported from the nucleus to cytoplasm contain leucine-rich nuclear export signals (NESs). NESs are recognized by transport receptors belonging to the importin-beta family of proteins. It has been shown that NES export is mediated by CRM 1 (Chromosome Region Maintenance 1), an importin-beta family member that binds NESs, Ran:GTP, and Ran binding protein (RanBP). CRM 1 mediates the nuclear exportation of numerous proteins, including histone deacetylases (HDACs), viral proteins, immunophilins, and STAT 1. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/5000. Detects a band of approximately 135 kDa (predicted molecular weight: 119 kDa).
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| 说明 |
|---|
|
WB
1/500 - 1/5000. Detects a band of approximately 135 kDa (predicted molecular weight: 119 kDa). |
靶标
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功能
Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of an nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap. Several viruses, among them HIV-1, HTLV-1 and influenza A use it to export their unspliced or incompletely spliced RNAs out of the nucleus. Interacts with, and mediates the nuclear export of HIV-1 Rev and HTLV-1 Rex proteins. Involved in HTLV-1 Rex multimerization. -
组织特异性
Expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Not expressed in the kidney. -
序列相似性
Belongs to the exportin family.
Contains 10 HEAT repeats.
Contains 1 importin N-terminal domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm. Nucleus > nucleoplasm. Nucleus > Cajal body. Nucleus > nucleolus. Located in the nucleoplasm, Cajal bodies and nucleoli. Shuttles between the nucleus/nucleolus and the cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 7514 Human
- Entrez Gene: 103573 Mouse
- Omim: 602559 Human
- SwissProt: O14980 Human
- SwissProt: Q6P5F9 Mouse
- Unigene: 370770 Human
- Unigene: 217547 Mouse
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别名
- Chromosome region maintenance 1 protein homolog antibody
- CRM 1 antibody
- CRM1 homolog antibody
see all
图片
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Western blot - Anti-CRM1 antibody (ab3459)All lanes : Anti-CRM1 antibody (ab3459) at 1/1000 dilution
Lane 1 : SK-BR-3 (Human mammary gland adenocarcinoma cell line) whole cell lysate
Lane 2 : HeLa (Human epithelial adenocarcinoma cell line) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit
Predicted band size: 119 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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Datasheet download
文献 (4)
ab3459 被引用在 4 文献中.
- Lussi YC et al. The nucleoporin Nup88 is interacting with nuclear lamin A. Mol Biol Cell 22:1080-90 (2011). PubMed: 21289091
- Castanotto D et al. CRM1 mediates nuclear-cytoplasmic shuttling of mature microRNAs. Proc Natl Acad Sci U S A 106:21655-9 (2009). IP ; Human . PubMed: 19955415
- Lai MC et al. The DEAD-box RNA helicase DDX3 associates with export messenger ribonucleoproteins as well as tip-associated protein and participates in translational control. Mol Biol Cell 19:3847-58 (2008). ICC/IF ; Human . PubMed: 18596238
- Mostaqul Huq MD et al. Suppression of receptor interacting protein 140 repressive activity by protein arginine methylation. EMBO J 25:5094-104 (2006). WB . PubMed: 17053781
