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Signal Transduction Protein Trafficking Nuclear Import / Export

Anti-CRM1抗体(ab3459)

  • Datasheet
Reviews (1) Submit a question References (4)

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Western blot - Anti-CRM1 antibody (ab3459)

    Key features and details

    • Rabbit polyclonal to CRM1
    • Suitable for: WB
    • Reacts with: Mouse, Human
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-CRM1 antibody [EPR14123(B)] (ab180144)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术,可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-CRM1抗体
      参阅全部 CRM1 一抗
    • 描述

      兔多克隆抗体to CRM1
    • 宿主

      Rabbit
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Mouse, Human
    • 免疫原

      Synthetic peptide corresponding to Human CRM1 aa 1043-1058.
      Sequence:

      RQADEEKHKRQMSVPGS


      (Peptide available as ab41768)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      Preservative: 0.05% Sodium azide
      Constituents: 0.1% BSA, 99% PBS
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • Primary antibody说明

      Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC’s. NPC’s contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Active transport of molecules through the NPC is a signal-mediated process that is not well understood. Proteins to be exported from the nucleus to cytoplasm contain leucine-rich nuclear export signals (NESs). NESs are recognized by transport receptors belonging to the importin-beta family of proteins. It has been shown that NES export is mediated by CRM 1 (Chromosome Region Maintenance 1), an importin-beta family member that binds NESs, Ran:GTP, and Ran binding protein (RanBP). CRM 1 mediates the nuclear exportation of numerous proteins, including histone deacetylases (HDACs), viral proteins, immunophilins, and STAT 1.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Signal Transduction
      • Protein Trafficking
      • Nuclear Import / Export
      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • RNA Processing
      • Other
      • Epigenetics and Nuclear Signaling
      • Nuclear Signaling Pathways
      • Nuclear Receptors
      • Nuclear Pore Complex

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human CRM1 protein (ab131897)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab3459于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    1/500 - 1/5000. Detects a band of approximately 135 kDa (predicted molecular weight: 119 kDa).
    说明
    WB
    1/500 - 1/5000. Detects a band of approximately 135 kDa (predicted molecular weight: 119 kDa).

    靶标

    • 功能

      Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of an nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap. Several viruses, among them HIV-1, HTLV-1 and influenza A use it to export their unspliced or incompletely spliced RNAs out of the nucleus. Interacts with, and mediates the nuclear export of HIV-1 Rev and HTLV-1 Rex proteins. Involved in HTLV-1 Rex multimerization.
    • 组织特异性

      Expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Not expressed in the kidney.
    • 序列相似性

      Belongs to the exportin family.
      Contains 10 HEAT repeats.
      Contains 1 importin N-terminal domain.
    • 翻译后修饰

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位

      Cytoplasm. Nucleus > nucleoplasm. Nucleus > Cajal body. Nucleus > nucleolus. Located in the nucleoplasm, Cajal bodies and nucleoli. Shuttles between the nucleus/nucleolus and the cytoplasm.
    • Target information above from: UniProt accession O14980 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 7514 Human
      • Entrez Gene: 103573 Mouse
      • Omim: 602559 Human
      • SwissProt: O14980 Human
      • SwissProt: Q6P5F9 Mouse
      • Unigene: 370770 Human
      • Unigene: 217547 Mouse
      • 别名

        • Chromosome region maintenance 1 protein homolog antibody
        • CRM 1 antibody
        • CRM1 homolog antibody
        • DKFZp686B1823 antibody
        • emb antibody
        • Exp 1 antibody
        • Exp1 antibody
        • Exportin 1 antibody
        • Exportin-1 antibody
        • Exportin1 antibody
        • XPO 1 antibody
        • xpo1 antibody
        • XPO1_HUMAN antibody
        see all

      图片

      • Western blot - Anti-CRM1 antibody (ab3459)
        Western blot - Anti-CRM1 antibody (ab3459)
        All lanes : Anti-CRM1 antibody (ab3459) at 1/1000 dilution

        Lane 1 : SK-BR-3 (Human mammary gland adenocarcinoma cell line) whole cell lysate
        Lane 2 : HeLa (Human epithelial adenocarcinoma cell line) whole cell lysate
        Lane 3 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate

        Lysates/proteins at 25 µg per lane.

        Secondary
        All lanes : HRP-conjugated anti-rabbit

        Predicted band size: 119 kDa
        Observed band size: 130 kDa why is the actual band size different from the predicted?

      实验方案

      • Western blot protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet download

        Download

      文献 (4)

      发表研究结果有使用 ab3459?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab3459 被引用在 4 文献中.

      • Lussi YC  et al. The nucleoporin Nup88 is interacting with nuclear lamin A. Mol Biol Cell 22:1080-90 (2011). PubMed: 21289091
      • Castanotto D  et al. CRM1 mediates nuclear-cytoplasmic shuttling of mature microRNAs. Proc Natl Acad Sci U S A 106:21655-9 (2009). IP ; Human . PubMed: 19955415
      • Lai MC  et al. The DEAD-box RNA helicase DDX3 associates with export messenger ribonucleoproteins as well as tip-associated protein and participates in translational control. Mol Biol Cell 19:3847-58 (2008). ICC/IF ; Human . PubMed: 18596238
      • Mostaqul Huq MD  et al. Suppression of receptor interacting protein 140 repressive activity by protein arginine methylation. EMBO J 25:5094-104 (2006). WB . PubMed: 17053781

      客户评价及客户问答

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      Immunocytochemistry abreview for Anti-CRM1 antibody

      Excellent
      Abreviews
      Abreviews
      Application
      Immunocytochemistry
      Sample
      Human Cell (HeLa)
      Specification
      HeLa
      Fixative
      Paraformaldehyde
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Sep 06 2006

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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