重组Anti-CrkL (phospho Y207)抗体[EP270Y] (ab52908)
![Western blot - Anti-CrkL (phospho Y207) antibody [EP270Y] (ab52908)](https://www.abcam.cn/ps/products/52/ab52908/Images/ab52908-65395-anti-crkl-phospho-y207-antibody-ep270y-western-blot.jpg "Western blot - Anti-CrkL (phospho Y207) antibody [EP270Y] (ab52908)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP270Y] to CrkL (phospho Y207)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CrkL (phospho Y207)抗体[EP270Y]
参阅全部 CrkL 一抗 -
描述
兔单克隆抗体[EP270Y] to CrkL (phospho Y207) -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt or IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: K562 cell lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP270Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52908于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/50000. Detects a band of approximately 39 kDa (predicted molecular weight: 34 kDa).
|
| 说明 |
|---|
|
WB
1/50000. Detects a band of approximately 39 kDa (predicted molecular weight: 34 kDa). |
应用说明
Is unsuitable for Flow Cyt or IHC-P.
靶标
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功能
May mediate the transduction of intracellular signals. -
序列相似性
Belongs to the CRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains. - Information by UniProt
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数据库链接
- Entrez Gene: 1399 Human
- Entrez Gene: 12929 Mouse
- Omim: 602007 Human
- SwissProt: P46109 Human
- SwissProt: P47941 Mouse
- Unigene: 5613 Human
- Unigene: 21048 Mouse
- Unigene: 451241 Mouse
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别名
- Crk L antibody
- Crk like protein antibody
- Crk-like protein antibody
see all
图片
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Western blot - Anti-CrkL (phospho Y207) antibody [EP270Y] (ab52908)All lanes : Anti-CrkL (phospho Y207) antibody [EP270Y] (ab52908) at 1/50000 dilution
Lane 1 : (A) K562 cell lysates untreated
Lane 2 : (B) K562 cell lysates treated with AP
Lysates/proteins at 10 µg per lane.
Predicted band size: 34 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted? -
![Anti-CrkL (phospho Y207) antibody [EP270Y] (ab52908)](https://www.abcam.cn/ps/products/52/ab52908/Images/ab52908-2-benefits-of-recombinant-antibodies.png)
Anti-CrkL (phospho Y207) antibody [EP270Y] (ab52908)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab52908 被引用在 6 文献中.
- Ouellette SB et al. A Cell-Based Assay for Measuring Endogenous BcrAbl Kinase Activity and Inhibitor Resistance. PLoS One 11:e0161748 (2016). PubMed: 27598410
- Li J et al. Inhibition of 32Dp210 cells harboring T315I mutation by a novel derivative of emodin correlates with down-regulation of BCR-ABL and its downstream signaling pathways. J Cancer Res Clin Oncol N/A:N/A (2014). Human . PubMed: 25217883
- Frietsch JJ et al. LASP1 is a novel BCR-ABL substrate and a phosphorylation-dependent binding partner of CRKL in chronic myeloid leukemia. Oncotarget 5:5257-71 (2014). WB . PubMed: 24913448
- Yang TY et al. A multiple reaction monitoring (MRM) method to detect Bcr-Abl kinase activity in CML using a peptide biosensor. PLoS One 8:e56627 (2013). WB ; Human . PubMed: 23437189
- Borgo C et al. Aberrant signalling by protein kinase CK2 in imatinib-resistant chronic myeloid leukaemia cells: biochemical evidence and therapeutic perspectives. Mol Oncol 7:1103-15 (2013). PubMed: 24012109
- Placzek EA et al. A peptide biosensor for detecting intracellular Abl kinase activity using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal Biochem 397:73-8 (2010). WB ; Human . PubMed: 19818327