重组Anti-CRISPR-associated endonuclease Cas12a抗体[3D3-F7] - BSA and Azide free (ab273478)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [3D3-F7] to CRISPR-associated endonuclease Cas12a - BSA and Azide free
- Suitable for: WB
Related conjugates and formulations
概述
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产品名称
Anti-CRISPR-associated endonuclease Cas12a抗体[3D3-F7] - BSA and Azide free
参阅全部 CRISPR-associated endonuclease Cas12a 一抗 -
描述
小鼠单克隆抗体[3D3-F7] to CRISPR-associated endonuclease Cas12a - BSA and Azide free -
宿主
Mouse -
特异性
Reacts with Acidaminococcus sp.
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经测试应用
适用于: WBmore details -
种属反应性
与反应: Acidaminococcus sp. -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HEK-293T transfected with AsCpf1 Cas12a expression vector containing a myc-His-tag®, whole cell lysate.
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常规说明
ab273478 is the carrier-free version of ab273438.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
Constituent: 100% PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
3D3-F7 -
同种型
IgG2a
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273478于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration.
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说明 |
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WB
Use at an assay dependent concentration. |
图片
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Western blot - Anti-CRISPR-associated endonuclease Cas12a antibody [3D3-F7] - BSA and Azide free (ab273478)All lanes : Anti-CRISPR-associated endonuclease Cas12a antibody [3D3-F7] (ab273438) at 1/1000 dilution
Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293T transfected with AsCpf1 Cas12a expression vector containing a myc-His-tag®, whole cell lysate
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 5 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Exposure time: 3 secondsThis image was produced using ab273438, the same clone but in a different formulation.
Blocking/Dilution buffer: 5% NFDM/TBST.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
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