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重组RabMAb

重组Anti-CRF抗体[EPR23255-162] (ab272391)

Submit a review Submit a question References (2)
Immunohistochemistry (Frozen sections) - Anti-CRF antibody [EPR23255-162] (ab272391)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRF antibody [EPR23255-162] (ab272391)
  • Immunohistochemistry (Frozen sections) - Anti-CRF antibody [EPR23255-162] (ab272391)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRF antibody [EPR23255-162] (ab272391)
  • Anti-CRF antibody [EPR23255-162] (ab272391)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23255-162] to CRF
  • Suitable for: IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 APC Carrier Free PE

概述

  • 产品名称

    Anti-CRF抗体[EPR23255-162]
    参阅全部 CRF 一抗

  • 描述

    兔单克隆抗体[EPR23255-162] to CRF

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-Fr, IHC-Pmore details
    不适用于: IP or WB

  • 种属反应性

    与反应: Mouse, Rat
    不与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Rat and mouse hypothalamus tissue. IHC-Fr: Rat and mouse hypothalamus tissue.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab272391于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-Fr
1/100.
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
IHC-Fr
1/100.
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
应用说明
Is unsuitable for IP or WB.

靶标

  • 相关性

    CRF precursor is a 41-amino acid peptide derived from a 196-amino acid preprohormone. CRF is a neuroendocrine hormone secreted by the paraventricular nucleus (PVN) of the hypothalamus and is a key player in the hypothalamic pituitary adrenal axis. It is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. Neuronal CRF is important in a variety of diverse roles including, memory, depression, stress, kidney function, parturition, metabolism and feeding (to mention a few).

  • 细胞定位

    Cytoplasmic and Secreted

  • 数据库链接

    • 别名

      • Corticoliberin antibody
      • Corticotropin releasing factor antibody
      • Corticotropin releasing hormone antibody
      • CRF antibody
      • Crh antibody
      • CRH1 antibody
      see all

    图片

    • Immunohistochemistry (Frozen sections) - Anti-CRF antibody [EPR23255-162] (ab272391)
      Immunohistochemistry (Frozen sections) - Anti-CRF antibody [EPR23255-162] (ab272391)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat hypothalamus tissue labeling CRF with ab272391 at /100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1000 (2 µg/ml) dilution (Green). Positive staining on median eminence of rat hypothalamus is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondaryat 1000 (2 µg/ml) dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRF antibody [EPR23255-162] (ab272391)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRF antibody [EPR23255-162] (ab272391)

      Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue labeling CRF with ab272391 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat hypothalamus is observed (PMID: 28883467). The section was incubated with ab272391 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Frozen sections) - Anti-CRF antibody [EPR23255-162] (ab272391)
      Immunohistochemistry (Frozen sections) - Anti-CRF antibody [EPR23255-162] (ab272391)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse hypothalamus tissue labeling CRF with ab272391 at /100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1000 (2 µg/ml) dilution (Green). Positive staining on median eminence of mouse hypothalamus is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondaryat 1000 (2 µg/ml) dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRF antibody [EPR23255-162] (ab272391)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRF antibody [EPR23255-162] (ab272391)

      Immunohistochemical analysis of paraffin-embedded mouse hypothalamus tissue labeling CRF with ab272391 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse hypothalamus is observed (PMID: 23724107). The section was incubated with ab272391 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Anti-CRF antibody [EPR23255-162] (ab272391)
      Anti-CRF antibody [EPR23255-162] (ab272391)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (2)

    发表研究结果有使用 ab272391？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab272391 被引用在 2 文献中.

    • Chang HC  et al. rAAV-CRISPRa therapy corrects Rai1 haploinsufficiency and rescues selective disease features in Smith-Magenis syndrome mice. J Biol Chem 299:102728 (2023). PubMed: 36410433
    • Sampedro-Piquero P  et al. Long-term consequences of alcohol use in early adolescent mice: Focus on neuroadaptations in GR, CRF and BDNF. Addict Biol 27:e13158 (2022). PubMed: 35229955

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