Anti-CREST抗体(ab227535)
Key features and details
- Rabbit polyclonal to CREST
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-CREST抗体
参阅全部 CREST 一抗 -
描述
兔多克隆抗体to CREST -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment within Human CREST (internal sequence). The exact sequence is proprietary.
Database link: O75177 -
阳性对照
- WB: Mouse brain lysate; HeLa whole cell and nuclear lysates. IP: MCF7 whole cell extract. IHC-P: Huh-7 xenograft tissue. ICC/IF: A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab227535于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 43 kDa.
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IP |
1/100 - 1/500.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 43 kDa. |
IP
1/100 - 1/500. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. -
组织特异性
Ubiquitous; with lowest levels in spleen. -
序列相似性
Belongs to the SS18 family. -
结构域
The MFD (multi-functional domain) domain is involved in transcription transactivation, nuclear body targeting and dimerization. -
细胞定位
Nucleus. Chromosome > centromere > kinetochore. Localizes to nuclear bodies. Co-localizes with SGOL1 at kinetochore. - Information by UniProt
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数据库链接
- Entrez Gene: 26039 Human
- Entrez Gene: 269397 Mouse
- Omim: 606472 Human
- SwissProt: O75177 Human
- SwissProt: Q8BW22 Mouse
- Unigene: 154429 Human
- Unigene: 63068 Mouse
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别名
- Calcium-responsive transactivator antibody
- CREST antibody
- CREST_HUMAN antibody
see all
图片
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Anti-CREST antibody (ab227535) at 1/1000 dilution + Mouse brain lysate at 50 µg
Predicted band size: 43 kDa10% SDS-PAGE gel.
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All lanes : Anti-CREST antibody (ab227535) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa nuclear lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 43 kDa10% SDS-PAGE gel.
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Paraffin-embedded Huh-7 xenograft tissue stained for CREST with ab227535 at 1/500 dilution in immunohistochemical analysis.
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Paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for CREST (green) using ab227535 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
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CREST was immunoprecipitated from MCF7 (human breast adenocarcinoma cell line) whole cell extract with 5 μg ab227535. Western blot was performed from the immunoprecipitate using ab227535.
Lane 1: Control IgG IP in MCF7 whole cell extract.
Lane 2: ab227535 IP in MCF7 whole cell extract.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab227535 被引用在 2 文献中.
- Jin X et al. RAB7 activity is required for the regulation of mitophagy in oocyte meiosis and oocyte quality control during ovarian aging. Autophagy 18:643-660 (2022). PubMed: 34229552
- Beon J et al. Inositol polyphosphate multikinase physically binds to the SWI/SNF complex and modulates BRG1 occupancy in mouse embryonic stem cells. Elife 11:N/A (2022). PubMed: 35551737