Anti-CREST抗体(ab126126)
Key features and details
- Rabbit polyclonal to CREST
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CREST抗体
参阅全部 CREST 一抗 -
描述
兔多克隆抗体to CREST -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow
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免疫原
Recombinant fragment within Human CREST aa 200-383. The exact sequence is proprietary.
Database link: O75177 -
阳性对照
- WB: MCF7 whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab126126 is purified by antigen-affinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126126于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 43 kDa.
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| 说明 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 43 kDa. |
靶标
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功能
Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. -
组织特异性
Ubiquitous; with lowest levels in spleen. -
序列相似性
Belongs to the SS18 family. -
结构域
The MFD (multi-functional domain) domain is involved in transcription transactivation, nuclear body targeting and dimerization. -
细胞定位
Nucleus. Chromosome > centromere > kinetochore. Localizes to nuclear bodies. Co-localizes with SGOL1 at kinetochore. - Information by UniProt
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数据库链接
- Entrez Gene: 26039 Human
- Entrez Gene: 269397 Mouse
- Entrez Gene: 192352 Rat
- Omim: 606472 Human
- SwissProt: O75177 Human
- SwissProt: Q8BW22 Mouse
- SwissProt: Q91XJ0 Rat
- Unigene: 154429 Human
see all -
别名
- Calcium-responsive transactivator antibody
- CREST antibody
- CREST_HUMAN antibody
see all
图片
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Western blot - Anti-CREST antibody (ab126126)Anti-CREST antibody (ab126126) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg
Predicted band size: 43 kDa
10% SDS PAGE
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab126126 尚未被引用在任何文献中。