Anti-CREBBP (acetyl K1535)抗体(ab61242)
Key features and details
- Rabbit polyclonal to CREBBP (acetyl K1535)
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CREBBP (acetyl K1535)抗体
参阅全部 CREBBP 一抗 -
描述
兔多克隆抗体to CREBBP (acetyl K1535) -
宿主
Rabbit -
特异性
Detects endogenous levels of CBP only when acetylated at lysine 1535. -
经测试应用
适用于: IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human CREBBP (acetyl K1535).
Sequence:SAKAcEL
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阳性对照
- Human lung carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from rabbit antiserum by affinity chromatography using epitope specific acetylated peptide. The antibody against non acetylated peptide was removed by chromatography using non acetylated peptide corresponding to the acetylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab61242于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50 - 1/100.
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| ICC/IF |
Use a concentration of 1 - 5 µg/ml.
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| 说明 |
|---|
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IHC-P
1/50 - 1/100. |
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ICC/IF
Use a concentration of 1 - 5 µg/ml. |
靶标
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功能
Acetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 coactivator. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1 in the presence of EP300. -
疾病相关
Note=Chromosomal aberrations involving CREBBP may be a cause of acute myeloid leukemias. Translocation t(8;16)(p11;p13) with MYST3/MOZ; translocation t(11;16)(q23;p13.3) with MLL/HRX; translocation t(10;16)(q22;p13) with MYST4/MORF. MYST3-CREBBP may induce leukemia by inhibiting RUNX1-mediated transcription.
Defects in CREBBP are a cause of Rubinstein-Taybi syndrome type 1 (RSTS1) [MIM:180849]. RSTS1 is an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies. -
序列相似性
Contains 1 bromo domain.
Contains 1 KIX domain.
Contains 2 TAZ-type zinc fingers.
Contains 1 ZZ-type zinc finger. -
结构域
The KIX domain mediates binding to HIV-1 Tat. -
翻译后修饰
Methylation of the KIX domain by CARM1 blocks association with CREB. This results in the blockade of CREB signaling, and in activation of apoptotic response.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Sumoylation negatively regulates transcriptional activity via the recruitment of DAAX. -
细胞定位
Cytoplasm. Nucleus. Recruited to nuclear bodies by SS18L1/CREST. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 1387 Human
- Entrez Gene: 12914 Mouse
- Entrez Gene: 54244 Rat
- Omim: 600140 Human
- SwissProt: Q92793 Human
- SwissProt: P45481 Mouse
- SwissProt: Q6JHU9 Rat
- Unigene: 459759 Human
see all -
别名
- CBP antibody
- CBP_HUMAN antibody
- CREB binding protein antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP (acetyl K1535) antibody (ab61242)
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using CREBBP (acetyl K1535) antibody (ab61242) at 1/50 dilution, in the presence (right panel) or absence (left panel) of acetylated peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-CREBBP (acetyl K1535) antibody (ab61242)ICC/IF image of ab61242 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61242, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab61242 被引用在 1 文献中.
- Huang YS et al. Cordyceps cicadae Prevents Renal Tubular Epithelial Cell Apoptosis by Regulating the SIRT1/p53 Pathway in Hypertensive Renal Injury. Evid Based Complement Alternat Med 2020:7202519 (2020). PubMed: 32419819