重组Anti-CREB3抗体[EPR14215(B)] (ab180119)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14215(B)] to CREB3
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CREB3抗体[EPR14215(B)]
参阅全部 CREB3 一抗 -
描述
兔单克隆抗体[EPR14215(B)] to CREB3 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human CREB3 aa 50-150. The exact sequence is proprietary.
Database link: O43889 -
阳性对照
- HeLa and 293 cells; HeLa, HepG2 and 293 cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR14215(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180119于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
ICC/IF |
1/100.
|
|
WB |
1/2000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 44 kDa).
|
说明 |
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Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/100. |
WB
1/2000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 44 kDa). |
靶标
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相关性
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element, an octameric palindrome. The protein interacts with host cell factor C1, which also associates with the herpes simplex virus (HSV) protein VP16 that induces transcription of HSV immediate-early genes. This protein and VP16 both bind to the same site on host cell factor C1. It is thought that the interaction between this protein and host cell factor C1 plays a role in the establishment of latency during HSV infection. An additional transcript variant has been identified, but its biological validity has not been determined. -
细胞定位
Isoform 2: Endoplasmic reticulum membrane; Single-pass type II membrane protein. Membrane. Isoform 3: Nucleus. Cytoplasm. -
数据库链接
- Entrez Gene: 10488 Human
- Omim: 606443 Human
- SwissProt: O43889 Human
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别名
- Basic leucine zipper protein antibody
- cAMP responsive element binding protein 3 antibody
- Clic AMP responsive element binding protein 3 antibody
see all
图片
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Anti-CREB3 antibody [EPR14215(B)] (ab180119) at 1/2000 dilution + HeLa cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 44 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
All lanes : Anti-CREB3 antibody [EPR14215(B)] (ab180119) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 44 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
Immunofluorescent analysis of 4% paraformaldehyde-fixed 293 cells labeling CREB3 with ab180119 at 1/100 dilution. Counterstained with DAPI (blue).
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Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cellslabeling CREB3 with ab180119 at 1/100 dilution (red) compared toa rabbit IgGcontrol (green), followed by secondaryGoat anti rabbit IgG (FITC) at 1/75 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (3)
ab180119 被引用在 3 文献中.
- Wang M et al. Golgi stress induces SIRT2 to counteract Shigella infection via defatty-acylation. Nat Commun 13:4494 (2022). PubMed: 35918380
- Li J et al. CircBACH1/let-7a-5p axis enhances the proliferation and metastasis of colorectal cancer by upregulating CREB5 expression. J Gastrointest Oncol 11:1186-1199 (2020). PubMed: 33456992
- Zhang M et al. LncRNA SNHG5 affects cell proliferation, metastasis and migration of colorectal cancer through regulating miR-132-3p/CREB5. Cancer Biol Ther 20:524-536 (2019). PubMed: 30395767