Anti-Creatine Kinase MM抗体(ab174672)
Key features and details
- Goat polyclonal to Creatine Kinase MM
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Creatine Kinase MM抗体
参阅全部 Creatine Kinase MM 一抗 -
描述
山羊多克隆抗体to Creatine Kinase MM -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Rabbit, Chicken, Cow, Dog, Pig
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免疫原
Synthetic peptide corresponding to Human Creatine Kinase MM aa 258-270 (internal sequence) (Cysteine residue). (NP_001815.2)
Sequence:QKIEEIFKKAGHP
Database link: P06732 -
阳性对照
- WB: Human and mouse heart lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab174672于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa).
A 1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa). A 1 hour primary incubation is recommended for this product. |
靶标
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功能
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. -
序列相似性
Belongs to the ATP:guanido phosphotransferase family.
Contains 1 phosphagen kinase C-terminal domain.
Contains 1 phosphagen kinase N-terminal domain. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 476435 Dog
- Entrez Gene: 1158 Human
- Entrez Gene: 12715 Mouse
- Entrez Gene: 24265 Rat
- Omim: 123310 Human
- SwissProt: P05123 Dog
- SwissProt: P06732 Human
- SwissProt: P07310 Mouse
see all -
别名
- CKM antibody
- CKMM antibody
- Creatine kinase M antibody
see all
图片
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Western blot - Anti-Creatine Kinase MM antibody (ab174672)All lanes : Anti-Creatine Kinase MM antibody (ab174672) at 0.03 µg/ml
Lane 1 : Human heart lysate (in RIPA buffer)
Lane 2 : Mouse heart lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 43 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab174672 被引用在 3 文献中.
- Fox CD et al. Frequent Manipulation of Resistance Training Variables Promotes Myofibrillar Spacing Changes in Resistance-Trained Individuals. Front Physiol 12:773995 (2021). PubMed: 34975527
- Ali HR et al. Defining decreased protein succinylation of failing human cardiac myofibrils in ischemic cardiomyopathy. J Mol Cell Cardiol 138:304-317 (2020). PubMed: 31836543
- Lynch JM et al. Mutant myocilin impacts sarcomere ultrastructure in mouse gastrocnemius muscle. PLoS One 13:e0206801 (2018). PubMed: 30395621