重组Anti-Creatine kinase B type抗体[EPR3927] (ab92452)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3927] to Creatine kinase B type
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Creatine kinase B type抗体[EPR3927]
参阅全部 Creatine kinase B type 一抗 -
描述
兔单克隆抗体[EPR3927] to Creatine kinase B type -
宿主
Rabbit -
特异性
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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经测试应用
适用于: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Creatine kinase B type aa 350-450 (C terminal). The exact sequence is proprietary.
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阳性对照
- WB: HeLa, HAP1, SHSY-5Y, Y-79, SW840, mouse cerebellum, mouse brain, C6 and rat brain whole cell lysates IHC-P: Human astrocytoma and glioma Tissue ICC/IF: HeLa cells Flow Cyt (intra): HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3927 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab92452于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 43 kDa.
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IHC-P |
1/100 - 1/1000.
See IHC antigen retrieval protocols. Use of an HRP/AP polymerized secondary antibody is recommended for enhanced staining. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). |
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ICC/IF |
1/50 - 1/500.
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
1/10000 - 1/50000. Predicted molecular weight: 43 kDa. |
IHC-P
1/100 - 1/1000. See IHC antigen retrieval protocols. Use of an HRP/AP polymerized secondary antibody is recommended for enhanced staining. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). |
ICC/IF
1/50 - 1/500. |
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. -
序列相似性
Belongs to the ATP:guanido phosphotransferase family.
Contains 1 phosphagen kinase C-terminal domain.
Contains 1 phosphagen kinase N-terminal domain. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 1152 Human
- Entrez Gene: 12709 Mouse
- Entrez Gene: 24264 Rat
- Omim: 123280 Human
- SwissProt: P12277 Human
- SwissProt: Q04447 Mouse
- SwissProt: P07335 Rat
- Unigene: 173724 Human
see all -
别名
- B CK antibody
- B-CK antibody
- BB-CK antibody
see all
图片
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Creatine kinase B knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: SH-SY5Y cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab92452 observed at 45 kDa. Red - loading control, ab18058, observed at 2X kDa.ab92452 (unpurified) was shown to specifically react with Creatine kinase B when ProteinX knockout samples were used. Wild-type and Creatine kinase B knockout samples were subjected to SDS-PAGE. ab92452 and ab18058 (loading control to vinculin) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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Anti-Creatine kinase B type antibody [EPR3927] (ab92452) at 1/10000 dilution (Purified) + Y79 (Human retinoblastoma retinoblastoma) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa -
Lanes 1-4 : Anti-Creatine kinase B type antibody [EPR3927] (ab92452) at 1/10000 dilution (Purified)
Lane 5 : Anti-Creatine kinase B type antibody [EPR3927] (ab92452) at 1/10000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Mouse cerebellum lysates
Lane 3 : Mouse brain lysates
Lane 4 : C6 (Rat glial tumor glial cell) whole cell lysates
Lane 5 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human astrocytoma tissue sections labeling Creatine kinase B type with purified ab92452 at 1/1000 dilution (0.101 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Creatine kinase B type with purified ab92452 at 1:50 dilution (2.0 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Creatine kinase B type with purified ab92452 at 1/20 dilution (5 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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All lanes : Anti-Creatine kinase B type antibody [EPR3927] (ab92452) at 1/10000 dilution (unpurified)
Lane 1 : SHSY-5Y cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Y-79 cell lysate
Lane 4 : SW840 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 43 kDa -
Paraffin embedded Human glioma tissue labelled with ab92452 (unpurified) at 1/100 dilution.
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Immunofluorescence analysis of HeLa cells labelled with ab92452 (unpurified) at 1/100 dilution.
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Overlay histogram showing HeLa cells stained with ab92452 (unpurified) (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92452, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab92452 被引用在 6 文献中.
- Zhou J et al. Proteomic Analyses Identify Differentially Expressed Proteins and Pathways Between Low-Risk and High-Risk Subtypes of Early-Stage Lung Adenocarcinoma and Their Prognostic Impacts. Mol Cell Proteomics 20:100015 (2021). PubMed: 33508502
- Kurth I et al. Therapeutic targeting of SLC6A8 creatine transporter suppresses colon cancer progression and modulates human creatine levels. Sci Adv 7:eabi7511 (2021). PubMed: 34613776
- Ellery SJ et al. The Effects of Early-Onset Pre-Eclampsia on Placental Creatine Metabolism in the Third Trimester. Int J Mol Sci 21:N/A (2020). PubMed: 31991880
- Kepchia D et al. Diverse proteins aggregate in mild cognitive impairment and Alzheimer's disease brain. Alzheimers Res Ther 12:75 (2020). PubMed: 32560738
- Ellery SJ et al. Placental creatine metabolism in cases of placental insufficiency and reduced fetal growth. Mol Hum Reprod 25:495-505 (2019). PubMed: 31323678
- Wang Z et al. Proteomic analysis reveals energy metabolic dysfunction and neurogenesis in the prefrontal cortex of a lipopolysaccharide-induced mouse model of depression. Mol Med Rep 13:1813-20 (2016). PubMed: 26718926