AB108388

重组Anti-Creatine kinase B type抗体[EPR3926]

Anti-Creatine kinase B type antibody [EPR3926]

RabMAb
Recombinant
KO Validated
了解详情

Be the first to review this product! Submit a review

(11 Publications)

Rabbit Recombinant Monoclonal Creatine kinase B type antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 11 publications.

查看别名

CKBB, CKB, Creatine kinase B-type, Brain creatine kinase, Creatine kinase B chain, Creatine phosphokinase B-type, B-CK, CPK-B

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine kinase B type antibody [EPR3926] (AB108388)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine kinase B type antibody [EPR3926] (AB108388)

ab108388, at 1/100 dilution, staining Creatine Kinase BB in Human heart by Immunohistochemistry, Paraffin-embedded tissue.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine kinase B type antibody [EPR3926] (AB108388)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cerebrum tissue sections labeling Creatine kinase B type with purified ab108388 at 1/500 dilution (1.14 µg/ml). Perform heat mediated antigen retrieval using Citrate buffer, pH 6.0. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Lab

Western blot - Anti-Creatine kinase B type antibody [EPR3926] (AB108388)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : Creatine kinase B knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : SH-SY5Y cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab108388 observed at 45 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab108388 was shown to specifically react with Creatine kinase B when Creatine kinase B knockout samples were used. Wild-type and Creatine kinase B knockout samples were subjected to SDS-PAGE. ab108388 and ab18058 (loading control to vinculin) were diluted 1/50000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Creatine kinase B type antibody [EPR3926] (ab108388)

Predicted band size: 43 kDa

false

Unknown

Western blot - Anti-Creatine kinase B type antibody [EPR3926] (AB108388)

All lanes:

Western blot - Anti-Creatine kinase B type antibody [EPR3926] (ab108388) at 1/50000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

SH SY5Y cell lysate at 10 µg

Lane 3:

Y79 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Predicted band size: 43 kDa

false

Lab

Western blot - Anti-Creatine kinase B type antibody [EPR3926] (AB108388)

All lanes:

Western blot - Anti-Creatine kinase B type antibody [EPR3926] (ab108388) at 1/50000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

Mouse brain lysates at 20 µg

Lane 3:

Rat brain lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 43 kDa

Observed band size: 43 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-Creatine kinase B type antibody [EPR3926] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR3926

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "For unpurified use at 1/100 - 1/250.The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000 - 1/200000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000 - 1/200000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000 - 1/200000", "WB-species-notes": "" } } }

style

left-column

产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Stable for 12 months at -20°C

style

left-column

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate) (PubMed : 8186255). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle : localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation (By similarity). During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work (By similarity).

See full target information CKB

style

left-column

文献 (11)

Recent publications for all applications. Explore the full list and refine your search

Mucosal immunology 16:817-825 PubMed37716510

2023

Fundamental role for the creatine kinase pathway in protection from murine colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline H T Hall,Jordi M Lanis,Alexander S Dowdell,Emily M Murphy,Geetha Bhagavatula,Rane M Neuhart,Kiranmayee Yenugudhati Vijaya Sai,Sean P Colgan

The EMBO journal 42:e111148 PubMed36843552

2023

A Zeb1/MtCK1 metabolic axis controls osteoclast activation and skeletal remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Lingxin Zhu,Yi Tang,Xiao-Yan Li,Samuel A Kerk,Costas A Lyssiotis,Wenqing Feng,Xiaoyue Sun,Geoffrey E Hespe,Zijun Wang,Marc P Stemmler,Simone Brabletz,Thomas Brabletz,Evan T Keller,Jun Ma,Jung-Sun Cho,Jingwen Yang,Stephen J Weiss

American journal of cancer research 12:4652-4665 PubMed36381321

2022

CKB affects human osteosarcoma progression by regulating the p53 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fengxin Zhou,Xinli Dou,Chenguang Li

Cell death & disease 12:1050 PubMed34741026

2021

Mitochondrial Drp1 recognizes and induces excessive mPTP opening after hypoxia through BAX-PiC and LRRK2-HK2.

Applications

Unspecified application

Species

Unspecified reactive species

Chenyang Duan,Lei Kuang,Chen Hong,Xinming Xiang,Jiancang Liu,Qinghui Li,Xiaoyong Peng,Yuanqun Zhou,Hongchen Wang,Liangming Liu,Tao Li

Neoplasia (New York, N.Y.) 23:1147-1165 PubMed34706306

2021

CKB inhibits epithelial-mesenchymal transition and prostate cancer progression by sequestering and inhibiting AKT activation.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Wang,Mohit Hulsurkar,Lijuan Zhuo,Jinbang Xu,Han Yang,Samira Naderinezhad,Lin Wang,Guoliang Zhang,Nanping Ai,Linna Li,Jeffrey T Chang,Songlin Zhang,Ladan Fazli,Chad J Creighton,Fang Bai,Michael M Ittmann,Martin E Gleave,Wenliang Li

Cells 10: PubMed34065411

2021

Establishing a 3D In Vitro Hepatic Model Mimicking Physiologically Relevant to In Vivo State.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun Kyoung Kang,Madina Sarsenova,Da-Hyun Kim,Min Soo Kim,Jin Young Lee,Eun-Ah Sung,Myung Geun Kook,Nam Gyo Kim,Soon Won Choi,Vyacheslav Ogay,Kyung-Sun Kang

Nature metabolism 2:62-80 PubMed32694686

2020

The creatine-phosphagen system is mechanoresponsive in pancreatic adenocarcinoma and fuels invasion and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Vassilis Papalazarou,Tong Zhang,Nikki R Paul,Amelie Juin,Marco Cantini,Oliver D K Maddocks,Manuel Salmeron-Sanchez,Laura M Machesky

Andrology 8:407-420 PubMed31364287

2019

Characterization of seminal plasma proteomic alterations associated with the IVF and rescue-ICSI pregnancy in assisted reproduction.

Applications

Unspecified application

Species

Unspecified reactive species

X Liu,G Liu,P Zhu,Y Wang,J Wang,W Zhang,W Wang,N Li,X Wang,C Zhang,J Liu,X Shen,F Liu

Oncology letters 18:1133-1144 PubMed31423173

2019

Cystic and solitary nodular pulmonary metastases in a patient with low-grade endometrial stromal sarcoma: A case report and literature review.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Xu,Zhi-Xin Liang,Jun-Tang Guo,Xin Su,Yun-Long Lu,Xi-Zhou Guan

Molecular reproduction and development 83:526-40 PubMed27088550

2016

Implications of caveolae in testicular and epididymal myoid cells to sperm motility.

Applications

Unspecified application

Species

Unspecified reactive species

Regiana L Oliveira,Adam Parent,Daniel G Cyr,Mary Gregory,Craig A Mandato,Charles E Smith,Louis Hermo

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-Creatine kinase B type抗体[EPR3926]