Anti-CRB3抗体[14F9] (ab180835)
![Immunocytochemistry/ Immunofluorescence - Anti-CRB3 antibody [14F9] (ab180835)](https://www.abcam.cn/ps/products/180/ab180835/Images/ab180835-209561-anti-crb3-antibody-14f9-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CRB3 antibody [14F9] (ab180835)")
Key features and details
- Rat monoclonal [14F9] to CRB3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG2c
概述
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产品名称
Anti-CRB3抗体[14F9] -
描述
大鼠单克隆抗体[14F9] to CRB3 -
宿主
Rat -
特异性
The antibody recognizes the full length CRB3 protein but not the extra cellular domain of CRB3. Recognize the overexpressed human form and the endogenous form in mouse.
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经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant full length protein corresponding to Rat CRB3.
Database link: 301112 -
阳性对照
- Mouse kidney and colon tissue lysates; HEK293T cells overexpressed with CRB3. Mouse intestine tissue. HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
PFHM culture media -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Lyophilized protein G purified in PBS pH7.4 -
克隆
单克隆 -
克隆编号
14F9 -
同种型
IgG2c -
研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180835于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 13 kDa.
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| ICC/IF |
1/100.
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| 说明 |
|---|
|
WB
1/1000. Predicted molecular weight: 13 kDa. |
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ICC/IF
1/100. |
靶标
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功能
Involved in the establishment of cell polarity in mammalian epithelial cells. Regulates the morphogenesis of tight junctions. -
组织特异性
Preferentially expressed in epithelial tissues. Expressed at high levels in lung, kidney, retina, colon and mammary glands. Expressed at moderate levels in liver, spleen, pancreas, placenta and prostate. -
结构域
The PDZ-binding motif is involved in the interactions with PARD6A and MPP5. -
细胞定位
Apical cell membrane. Cell junction > tight junction. Localizes primarily to the apical membrane with a small fraction in the upper part of tight junctions of epithelial cells. - Information by UniProt
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数据库链接
- Entrez Gene: 92359 Human
- Entrez Gene: 224912 Mouse
- Omim: 609737 Human
- SwissProt: Q9BUF7 Human
- SwissProt: Q8QZT4 Mouse
- Unigene: 150319 Human
- Unigene: 391027 Mouse
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别名
- CRB3 antibody
- CRUM3_HUMAN antibody
- crumbs homolog 3 (Drosophila) antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-CRB3 antibody [14F9] (ab180835)
Immunofluorescence analysis of HeLa cell labeling CRB3 with ab1808350 at a 1/100 dilution. DAPI was used to stain the nuclei.
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![Western blot - Anti-CRB3 antibody [14F9] (ab180835)](https://www.abcam.cn/ps/products/180/ab180835/Images/ab180835-209559-anti-crb3-antibody-14f9-western-blot.png)
Western blot - Anti-CRB3 antibody [14F9] (ab180835)All lanes : Anti-CRB3 antibody [14F9] (ab180835) at 1/1000 dilution
Lane 1 : HEK293T cells overexpressed with control vector
Lane 2 : HEK293T cells overexpressed with CRB3
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse colon tissue lysate
Predicted band size: 13 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-CRB3 antibody [14F9] (ab180835)](https://www.abcam.cn/ps/products/180/ab180835/Images/ab180835-209560-anti-crb3-antibody-14f9-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CRB3 antibody [14F9] (ab180835)Immunofluorescence analysis of Mouse intestine labeling CRB3 with ab1808350 at a 1/100 dilution. E-cadherin staining was used to highlight the lateral membrane in mouse intestine.
实验方案
数据表及文件
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Datasheet download
文献 (4)
ab180835 被引用在 4 文献中.
- McFaline-Figueroa JL et al. A pooled single-cell genetic screen identifies regulatory checkpoints in the continuum of the epithelial-to-mesenchymal transition. Nat Genet 51:1389-1398 (2019). PubMed: 31477929
- Feldner A et al. Loss of Mpdz impairs ependymal cell integrity leading to perinatal-onset hydrocephalus in mice. EMBO Mol Med 9:890-905 (2017). ICC/IF ; Mouse . PubMed: 28500065
- Tapia R et al. EPEC effector EspF promotes Crumbs3 endocytosis and disrupts epithelial cell polarity. Cell Microbiol 19:N/A (2017). PubMed: 28618099
- Huang C et al. Analysis of different components in the peritumoral tissue microenvironment of colorectal cancer: A potential prospect in tumorigenesis. Mol Med Rep 14:2555-65 (2016). IHC . PubMed: 27484148
