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重组RabMAb

重组Anti-CRALBP抗体[EPR23448-119] - BSA and Azide free (ab272171)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)
  • Flow Cytometry (Intracellular) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)
  • Immunoprecipitation - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)
  • Immunohistochemistry (Frozen sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)
  • Immunohistochemistry (Frozen sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)
  • Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23448-119] to CRALBP - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-Fr, IP, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-CRALBP抗体[EPR23448-119] - BSA and Azide free
    参阅全部 CRALBP 一抗

  • 描述

    兔单克隆抗体[EPR23448-119] to CRALBP - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), IHC-Fr, IP, WB, IHC-Pmore details
    不适用于: ICC/IF

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Human eyeball tissue lysate; Mouse eyeball tissue and P20 retina lysates; Rat eyeball tissue lysate; SK-MEL-2 and SK-MEL-28 whole cell lysates. IHC-P: Rat retina tissue, Mouse retina tissue; Human retina tissue. IHC-Fr: Mouse retina tissue; Rat retina tissue. Flow Cyt (intra): SK-MEL-28 cells. IP: SK-MEL-28 whole cell lysate.

  • 常规说明

    ab272171 is the carrier-free version of ab243664.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab272171于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
说明
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
应用说明
Is unsuitable for ICC/IF.

靶标

  • 功能

    Soluble retinoid carrier essential the proper function of both rod and cone photoreceptors. Participates in the regeneration of active 11-cis-retinol and 11-cis-retinaldehyde, from the inactive 11-trans products of the rhodopsin photocycle and in the de novo synthesis of these retinoids from 11-trans metabolic precursors. The cycling of retinoids between photoreceptor and adjacent pigment epithelium cells is known as the 'visual cycle'.

  • 组织特异性

    Retina and pineal gland. Not present in photoreceptor cells but is expressed abundantly in the adjacent retinal pigment epithelium (RPE) and in the Mueller glial cells of the retina.

  • 疾病相关

    Defects in RLBP1 are a cause of retinitis pigmentosa autosomal recessive (ARRP) [MIM:268000]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
    Defects in RLBP1 are the cause of Bothnia retinal dystrophy (BRD) [MIM:607475]; also known as Vasterbotten dystrophy. Affected individuals show night blindness from early childhood with features consistent with retinitis punctata albescens and macular degeneration.
    Defects in RLBP1 are the cause of rod-cone dystrophy Newfoundland (NFRCD) [MIM:607476]. NFRCD is a retinal dystrophy reminiscent of retinitis punctata albescens but with a substantially lower age at onset and more-rapid and distinctive progression. Rod-cone dystrophies results from initial loss of rod photoreceptors, later followed by cone photoreceptors loss.
    Defects in RLBP1 are a cause of fundus albipunctatus (FA) [MIM:136880]. FA is a rare form of stationary night blindness characterized by a delay in the regeneration of cone and rod photopigments.

  • 序列相似性

    Contains 1 CRAL-TRIO domain.

  • 细胞定位

    Cytoplasm.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Cellular retinaldehyde binding protein 1 antibody
      • Cellular retinaldehyde binding protein antibody
      • Cellular retinaldehyde-binding protein antibody
      • MGC3663 antibody
      • Retinaldehyde binding protein 1 antibody
      • Retinaldehyde-binding protein 1 antibody
      • RLBP 1 antibody
      • RLBP1 antibody
      • RLBP1_HUMAN antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)

      Immunohistochemical analysis of paraffin-embedded Human retina tissue labeling CRALBP with ab243664 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human retina. The section was incubated with ab243664 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243664).

    • Flow Cytometry (Intracellular) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)
      Flow Cytometry (Intracellular) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SK-MEL-28 (Human malignant melanoma) cells labelling CRALBP with ab243664 at 1/700 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor®488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243664). 

       

       

    • Immunoprecipitation - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)
      Immunoprecipitation - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)

      CRALBP was immunoprecipitated from 0.35 mg SK-MEL-28 (human malignant melanoma), whole cell lysate with ab243664 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab243664 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: SK-MEL-28 (human malignant melanoma), whole cell lysate 10 ug

      Lane 2: ab243664 IP in SK-MEL-28 whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab243664 in SK-MEL-28 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 100 seconds

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243664).

    • Immunohistochemistry (Frozen sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)
      Immunohistochemistry (Frozen sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse retina tissue labeling CRALBP with ab243664 at 250 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1000 dilution (Green). Positive staining on mouse retina especially on retinal pigment epithelial cell (RPE) and Muller cells. is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondaryat 1000 dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243664).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)

      Immunohistochemical analysis of paraffin-embedded Rat retina tissue labeling CRALBP with ab243664 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat retina. The section was incubated with ab243664 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243664).

    • Immunohistochemistry (Frozen sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)
      Immunohistochemistry (Frozen sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat retina tissue labeling CRALBP with ab243664 at 250 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1000 dilution (Green). Positive staining on rat retina especially on retinal pigment epithelial cell (RPE) and Muller cells. is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243664).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)

      Immunohistochemical analysis of paraffin-embedded Mouse retina tissue labeling CRALBP with ab243664 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse retina. The section was incubated with ab243664 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243664).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CRALBP with ab243664 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on human kidney. The section was incubated with ab243664 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243664).

    • Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)
      Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab272171？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab272171 尚未被引用在任何文献中。

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