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AB15051

Anti-CRALBP 抗体 [B2]

Anti-CRALBP antibody [B2]

4

(11 Reviews)

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(121 Publications)

Anti-CRALBP antibody [B2] (ab15051) is a mouse monoclonal antibody detecting CRALBP in Western Blot, IHC-P. Suitable for Mouse, Rat.

- Over 100 publications
- Trusted since 2004

查看别名

CRALBP, RLBP1, Retinaldehyde-binding protein 1, Cellular retinaldehyde-binding protein

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [B2] (AB15051)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [B2] (AB15051)

Immunohistochemistry image of CRALBP staining in a section of formalin-fixed paraffin-embedded normal mouse eye performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab15051, 0.5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Western blot - Anti-CRALBP antibody [B2] (AB15051)
  • WB

Project7942****

Western blot - Anti-CRALBP antibody [B2] (AB15051)

All lanes:

Western blot - Anti-CRALBP antibody [B2] (ab15051) at 1 µg/mL

Lane 1:

Western blot - Rat eye tissue lysate - total protein (<a href='/products/tissue-lysates/rat-eye-tissue-lysate-total-protein-ab4035'>ab4035</a>) at 10 µg

Lane 2:

Rat retina tissue lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 36 kDa

Observed band size: 35 kDa

true

Exposure time: 30s

不同偶联物与剂型 (1)

  • HRP

    HRP Anti-CRALBP antibody [B2]

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

B2

亚型

IgG1

轻链类型

kappa

不含载体蛋白

No

反应种属

Rat, Mouse

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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产品详情

What is this antibody validated in?
Anti-CRALBP antibody [B2] (ab15051) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Mouse, Rat samples.

What is the molecular weight of CRALBP?
Anti-CRALBP [B2] (ab15051) specifically detects a band for CRALBP (UniProt: P12271) at a molecular weight of 36kDa.

Trusted by the scientific community
Anti-CRALBP [B2] (ab15051) was first used in a scientific publication in 2004 and has been cited over 100 times in peer-reviewed journals.

Reviewed by scientists
Anti-CRALBP [B2] (ab15051) has over 5 independent reviews from customers.

Other related products
We have a range of other formats of antibody clone [B2] also available for your convenience: ab15051, HRP - ab199992, FITC - ab323746

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

'CRALBP' also known as cellular retinaldehyde-binding protein is a protein involved in the visual cycle. It weighs approximately 36 kDa. Expressed mainly in the retina CRALBP is found in retinal pigment epithelial (RPE) cells and Muller glial cells. It binds to retinoids which are critical for proper vision functioning. CRALBP also goes by the synonyms such as 'BSA b 2' which is used in specific contexts related to its role in retinal metabolism.
Biological function summary

The protein aids in the effective conversion of all-trans-retinal to 11-cis-retinal an important step in the visual cycle. It acts as a chaperone that stabilizes retinoid intermediates facilitating their transport between various retinal cell types. CRALBP forms part of a complex with retinoid isomerohydrolase and 11-cis-retinol dehydrogenase enhancing its binding and transport function. This ensures smooth retinoid flux necessary for regenerating the visual pigment rhodopsin.

Pathways

CRALBP is integral to two main pathways; the retinoid cycle in cones and the rhodopsin regeneration pathway in rods. In these pathways it interacts with proteins such as RPE65 and 11-cis-retinol dehydrogenase ensuring efficient retinoid transfer and isomerization. This important involvement supports the regeneration of visual pigments necessary for photoreceptor cell function under various lighting conditions.

CRALBP associates with retina-related conditions notably congenital stationary night blindness (CSNB) and retinitis pigmentosa (RP). Mutations affecting CRALBP can alter retinoid transport and contribute to these disorders. Additionally CRALBP dysfunction links to other proteins like RPE65 and RDH5 which can also play roles in the pathogenesis of these visual impairments. Understanding these relationships helps in developing targeted treatments for retinoid-related visual disorders.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Soluble retinoid carrier essential the proper function of both rod and cone photoreceptors. Participates in the regeneration of active 11-cis-retinol and 11-cis-retinaldehyde, from the inactive 11-trans products of the rhodopsin photocycle and in the de novo synthesis of these retinoids from 11-trans metabolic precursors. The cycling of retinoids between photoreceptor and adjacent pigment epithelium cells is known as the 'visual cycle'.
See full target information RLBP1
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文献 (121)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40943594

2025

Human Retinal Organoid Modeling Defines Developmental Window and Therapeutic Vulnerabilities in MYCN-Amplified Retinoblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jinkyu Park,Gang Cui,Jiyun Hong,Han Jeong,Minseok Han,Min Seok Choi,Jeong Ah Lim,Sanguk Han,Christopher Seungkyu Lee,Min Kim,Sangwoo Kim,Junwon Lee,Suk Ho Byeon

Materials today. Bio 32:101880 PubMed40520565

2025

Transplantation of human embryonic stem cell-derived retinal pigment epithelial cells via injectable microfluidic-templated microgels for retinal regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Wei,Cong Ma,Chuanfeng An,Yujie Zhang,Haoyue Zhang,Qiyou Li,Li Kong,Huanan Wang,Xiang Ma

Methods in molecular biology (Clifton, N.J.) 2924:101-112 PubMed40307638

2025

Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelial Cells for the Study of Macular Degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Almar Neiteler,Shyamanga Borooah,James A Ross

Investigative ophthalmology & visual science 66:53 PubMed40131295

2025

Neuronal Degeneration and Glial Activation in the Absence of Vascular Changes in Human Retinas of Patients With Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Henar Albertos-Arranz,Natalia Martínez-Gil,Xavier Sánchez-Sáez,Julio Cesar Molina-Martín,Pedro Lax,Nicolas Cuenca

International journal of molecular sciences 26: PubMed39795914

2025

Conventional and Tropism-Modified High-Capacity Adenoviral Vectors Exhibit Similar Transduction Profiles in Human iPSC-Derived Retinal Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew McDonald,Carmen Gallego,Charlotte Andriessen,Michaela Orlová,Manuel A F V Gonçalves,Jan Wijnholds

Scientific reports 15:412 PubMed39747958

2025

PEGDA-based HistoBrick for increasing throughput of cryosectioning and immunohistochemistry in organoid and small tissue studies.

Applications

Unspecified application

Species

Unspecified reactive species

Emilie Vuille-Dit-Bille,Larissa Utz,Fiona E Müllner,Valeria J Arteaga-Moreta,Yanyan Hou,Stefan E Spirig,Diane Ledroit-Paic,Sarah Heub,Jonas Goldowsky,Gilles Weder,Magdalena Renner

Current biology : CB 35:109-120.e4 PubMed39689705

2024

Layer-specific anatomical and physiological features of the retina's neurovascular unit.

Applications

Unspecified application

Species

Unspecified reactive species

William N Grimes,David M Berson,Adit Sabnis,Mrinalini Hoon,Raunak Sinha,Hua Tian,Jeffrey S Diamond

Investigative ophthalmology & visual science 65:23 PubMed39535788

2024

Genetic and Cellular Basis of Impaired Phagocytosis and Photoreceptor Degeneration in CLN3 Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jimin Han,Sueanne Chear,Jana Talbot,Vicki Swier,Clarissa Booth,Cheyenne Reuben-Thomas,Sonal Dalvi,Jill M Weimer,Alex W Hewitt,Anthony L Cook,Ruchira Singh

JCI insight 9: PubMed39171529

2024

Evaluating precision medicine approaches for gene therapy in patient-specific cellular models of Bietti crystalline dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Li,Richard R Yang,Yong-Shi Li,Chun-Wei Hsu,Laura A Jenny,Yang Kong,Merry Zc Ruan,Janet R Sparrow,Stephen H Tsang

Stem cell research & therapy 14:346 PubMed38049871

2023

CRX haploinsufficiency compromises photoreceptor precursor translocation and differentiation in human retinal organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Deng Pan,Xiao Zhang,Kangxin Jin,Zi-Bing Jin
View all publications
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