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Anti-CPT2抗体[1C2AE6] (ab110293)

Reviews (1) Submit a question References (7)
Immunocytochemistry/ Immunofluorescence - Anti-CPT2 antibody [1C2AE6] (ab110293)
  • Flow Cytometry - Anti-CPT2 antibody [1C2AE6] (ab110293)
  • Immunoprecipitation - Anti-CPT2 antibody [1C2AE6] (ab110293)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [1C2AE6] (ab110293)

Key features and details

  • Mouse monoclonal [1C2AE6] to CPT2
  • Suitable for: IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-CPT2抗体[1C2AE6]
    参阅全部 CPT2 一抗

  • 描述

    小鼠单克隆抗体[1C2AE6] to CPT2

  • 宿主

    Mouse

  • 经测试应用

    适用于: IHC-P, ICC/IF, Flow Cyt, IPmore details
    不适用于: WB

  • 种属反应性

    与反应: Mouse, Rat, Cow, Human

  • 免疫原

    Synthetic peptide. This information is considered to be commercially sensitive.

  • 阳性对照

    • HepG2 cells. HL-60 cells. Human normal colon FFPE tissue

  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab110293于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use a concentration of 10 µg/ml.
ICC/IF
Use a concentration of 4 µg/ml.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP
Use at an assay dependent concentration.
说明
IHC-P
Use a concentration of 10 µg/ml.
ICC/IF
Use a concentration of 4 µg/ml.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP
Use at an assay dependent concentration.
应用说明
Is unsuitable for WB.

靶标

  • 通路

    Lipid metabolism; fatty acid beta-oxidation.

  • 疾病相关

    Defects in CPT2 are the cause of carnitine palmitoyltransferase 2 deficiency (CPT2D) [MIM:255110, 600649]; also known as CPT-II deficiency or CPT2 deficiency. CPT2D is an autosomal recessive disorder characterized by recurrent myoglobinuria, episodes of muscle pain, stiffness, and rhabdomyolysis. These symptoms are triggered by prolonged exercise, fasting or viral infection and patients are usually young adults. In addition to this classical, late-onset, muscular type, a hepatic or hepatocardiomuscular form has been reported in infants. Clinical pictures in these children or neonates include hypoketotic hypoglycemia, liver dysfunction, cardiomyopathy and sudden death.
    Defects in CPT2 are the cause of carnitine palmitoyltransferase 2 deficiency, lethal neonatal (CPT2D-LN) [MIM:608836]; also known as lethal neonatal CPT-II deficiency. It is a lethal neonatal form of CPT2D. This rarely presentation is antenatal with cerebral periventricular cysts and cystic dysplastic kidneys. The clinical variability of the disease is likely attributed to the variable residual enzymatic activity.

  • 序列相似性

    Belongs to the carnitine/choline acetyltransferase family.

  • 细胞定位

    Mitochondrion inner membrane.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Carnitine O palmitoyltransferase 2 antibody
    • Carnitine O palmitoyltransferase 2 mitochondrial antibody
    • Carnitine O-palmitoyltransferase 2 antibody
    • Carnitine palmitoyltransferase 2 antibody
    • Carnitine palmitoyltransferase II antibody
    • CPT 1 antibody
    • CPT 2 antibody
    • CPT II antibody
    • CPT1 antibody
    • CPT2 antibody
    • CPT2_HUMAN antibody
    • CPTASE antibody
    • CPTII antibody
    • IIAE4 antibody
    • mitochondrial antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-CPT2 antibody [1C2AE6] (ab110293)
    Immunocytochemistry/ Immunofluorescence - Anti-CPT2 antibody [1C2AE6] (ab110293)
    Immunocytochemistry image of ab110293 stained Human HepG2 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with ab110293 at 4 µg/ml for 2h at room temperature, or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
  • Flow Cytometry - Anti-CPT2 antibody [1C2AE6] (ab110293)
    Flow Cytometry - Anti-CPT2 antibody [1C2AE6] (ab110293)
    HL-60 cells were stained with 1 µg/mL of ab110293 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • Immunoprecipitation - Anti-CPT2 antibody [1C2AE6] (ab110293)
    Immunoprecipitation - Anti-CPT2 antibody [1C2AE6] (ab110293)

    SDS-PAGE analysis of IP samples. As an IP antibody, ab110293 works with human, bovine, rat, and mouse samples.

    Lane STD: Molecular weight ladder

    Lane HLM: Human liver mitochondria

    Lane BHM: Bovine heart mitochondria

    Lane RLM: Rat liver mitochondria

    Lane MLM: Mouse liver mitochondria

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [1C2AE6] (ab110293)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [1C2AE6] (ab110293)

    IHC image of CPT2 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110293, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

文献 (7)

发表研究结果有使用 ab110293？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab110293 被引用在 7 文献中.

  • Mao Y  et al. Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation. Cell Res 34:13-30 (2024). PubMed: 38163844
  • Ma B  et al. Albumosomes formed by cytoplasmic pre-folding albumin maintain mitochondrial homeostasis and inhibit nonalcoholic fatty liver disease. Signal Transduct Target Ther 8:229 (2023). PubMed: 37321990
  • Lei W  et al. Notoginsenoside R1 Regulates Ischemic Myocardial Lipid Metabolism by Activating the AKT/mTOR Signaling Pathway. Front Pharmacol 13:905092 (2022). PubMed: 35814216
  • Liu L  et al. Low-Level Saturated Fatty Acid Palmitate Benefits Liver Cells by Boosting Mitochondrial Metabolism via CDK1-SIRT3-CPT2 Cascade. Dev Cell 52:196-209.e9 (2020). PubMed: 31866205
  • Zhang L  et al. The Protective Activities of Dietary Sea Cucumber Cerebrosides against Atherosclerosis through Regulating Inflammation and Cholesterol Metabolism in Male Mice. Mol Nutr Food Res 62:e1800315 (2018). PubMed: 29883529
  • Jernberg JN  et al. Developmental regulation and localization of carnitine palmitoyltransferases (CPTs) in rat brain. J Neurochem 142:407-419 (2017). PubMed: 28512781
  • Townsend KL  et al. Increased Mitochondrial Activity in BMP7-Treated Brown Adipocytes, Due to Increased CPT1- and CD36-Mediated Fatty Acid Uptake. Antioxid Redox Signal : (2012). PubMed: 22938691

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Western blot abreview for Anti-CPT2/CPT1 antibody [1C2AE6]

 Poor
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Treatment
Wild type and Knockout mouse tissue
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Apr 02 2018

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