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Anti-CPT1A抗体(ab53532)

Submit a review Q&A (3)References (4)
Western blot - Anti-CPT1A antibody (ab53532)

    Key features and details

    • Goat polyclonal to CPT1A
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术，可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-CPT1A抗体
      参阅全部 CPT1A 一抗

    • 描述

      山羊多克隆抗体to CPT1A

    • 宿主

      Goat

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Human
      预测可用于: Chimpanzee, Rhesus monkey

    • 免疫原

      Synthetic peptide:

      C-DPAQTVEQRLKLFK

      , corresponding to internal sequence amino acids 621-634 of Human CPT1A
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 阳性对照

      • Human Liver lysate

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab53532于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 86 kDa).

    1 hour primary incubation is recommended for this product.
    说明
    WB
    Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 86 kDa).

    1 hour primary incubation is recommended for this product.

    靶标

    • 组织特异性

      Strong expression in kidney and heart, and lower in liver and skeletal muscle.

    • 通路

      Lipid metabolism; fatty acid beta-oxidation.

    • 疾病相关

      Defects in CPT1A are the cause of carnitine palmitoyltransferase 1A deficiency (CPT1AD) [MIM:255120]; also known as CPT-I deficiency or CPT1A deficiency. CPT1AD is a rare autosomal recessive metabolic disorder of long-chain fatty acid oxidation characterized by severe episodes of hypoketotic hypoglycemia usually occurring after fasting or illness. Onset is in infancy or early childhood.

    • 序列相似性

      Belongs to the carnitine/choline acetyltransferase family.

    • 细胞定位

      Mitochondrion outer membrane.
    • Information by UniProt

    • 数据库链接

      • 别名

        • Carnitine O palmitoyltransferase 1 liver isoform antibody
        • Carnitine O palmitoyltransferase I antibody
        • Carnitine O palmitoyltransferase I liver isoform antibody
        • Carnitine O-palmitoyltransferase 1 antibody
        • Carnitine O-palmitoyltransferase I antibody
        • Carnitine palmitoyltransferase 1A (liver) antibody
        • Carnitine palmitoyltransferase 1A antibody
        • Carnitine palmitoyltransferase I antibody
        • Carnitine palmitoyltransferase I liver antibody
        • CPT 1 antibody
        • CPT I antibody
        • CPT1 antibody
        • CPT1 L antibody
        • CPT1-L antibody
        • Cpt1a antibody
        • CPT1A_HUMAN antibody
        • CPTI antibody
        • CPTI-L antibody
        • L CPT1 antibody
        • liver isoform antibody
        see all

      图片

      • Western blot - Anti-CPT1A antibody (ab53532)
        Western blot - Anti-CPT1A antibody (ab53532)
        Anti-CPT1A antibody (ab53532) at 0.1 µg/ml + Human Liver lysate at 35 µg

        Predicted band size: 86 kDa
        Observed band size: 80 kDa why is the actual band size different from the predicted?



        Primary incubation was 1 hour. Detected by chemiluminescence. RIPA buffer used. 

      文献 (4)

      发表研究结果有使用 ab53532？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab53532 被引用在 4 文献中.

      • Shatoor AS  et al. Short-term administration of C. aronia stimulates insulin signaling, suppresses fatty acids metabolism, and increases glucose uptake and utilization in the hearts of healthy rats. Saudi J Biol Sci 28:1966-1977 (2021). PubMed: 33732083
      • Nyandwi JB  et al. Rosmarinic Acid Exhibits a Lipid-Lowering Effect by Modulating the Expression of Reverse Cholesterol Transporters and Lipid Metabolism in High-Fat Diet-Fed Mice. Biomolecules 11:N/A (2021). PubMed: 34680102
      • Mazibuko-Mbeje SE  et al. Aspalathin-Enriched Green Rooibos Extract Reduces Hepatic Insulin Resistance by Modulating PI3K/AKT and AMPK Pathways. Int J Mol Sci 20:N/A (2019). PubMed: 30717198
      • Samudio I  et al. Pharmacologic inhibition of fatty acid oxidation sensitizes human leukemia cells to apoptosis induction. J Clin Invest 120:142-56 (2010). WB ; Human . PubMed: 20038799

      客户评价及客户问答

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      1-3 of 3 Abreviews or Q&A

      Question

      I will attempt to answer your questions below:
      1. What's the species you were using to detect the proteins: human,
      mouse, etc. proteins with a tag? HEK-293 lysates (human clones with
      N-terminal V5-tag). Some abs from abcam (ab60332, ab80464) worked well.
      2. Almost all of our antibodies are developed to work under denatured
      conditions or otherwise it will be stated on the datasheet. It may be
      that the antibody cannot detect its epitope as it may be simply not
      accessible. Please boil your samples to provide optimal conditions.
      The same goes for the reducing conditions of the gel which are
      usually necessary for the most our antibodies to work properly.
      I have tried denatured conditions are results were the same!
      3. In order to avoid over-loading and therefore to decrease the side
      bands, I would like to suggest loading 20 to 30 ug protein per lane.
      I have loaded 20-40 ug protein per lane.
      3. What are the listed primaries antibodies concentration? Or do you
      use 1:5000 for all of them?
      As you know, the primary antibody concentranctions vary, for instance:
      ab15575 (1:5000), ab53532 (1:1000), ab96615 (1:1000) and ab106460 (1:1000)
      4. What kind of secondary antibodies are you using for the listed
      primaries? Do you perform a non-primary control with them beforehand?
      depending on primary antibody used, secondary antibodies were: goat
      anti-mouse IgGHRP (170-6516), goat anti-rabbit IgGHRP (170-6515) and
      rabbit anti-goat IgGHRP (172-1034) from biorad.
      5. Is it possible to send us an image?
      6. How did you store the antibodies?
      antibodies are stored as recommended, usually -20 C or sometimes 4C
      P.s. note that I have already tested these antibodies at least 3 times
      over the last 4 months. Hope to get replacement aliquots shortly.

      Read More
      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

      I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused.

      As I have mentioned before, this two orders are way out of our Abpromise. However, I can see from the details you kindly provided the antibodies should have worked under these conditions, and I am therefore willing to make an exception in this case.


      To check the status of the order please contact our Customer Service team and reference this number.

      Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

      I wish you the best of luck with your research.

      Read More

      Question

      Please find the WB questionnaire below:
      1) Abcam product code ab: (ab15575, ab53532, ab96615 and ab106460)

      3) Description of the problem: the mentioned antibodies are
      non-specific i.e. several bands appear, none of which is the right size.
      4) Sample preparation:
      Type of sample (whole cell lysates, fraction, recombinant protein…):
      cytoplasmic extract
      Lysis buffer : Lysis buffer (50 mM HEPES, pH 7.4, 150 mM NaCl, 1 mM
      EDTA, 1% Nonidet P-40, 1 mM DTT and 10% glycerol) supplemented with
      benzonase nuclease (250U, E1014, Sigma),
      Protease inhibitors: 1 mM phenylmethylsulfonyl fluoride (P7626, Sigma)
      and 1X protease inhibitor cocktail (Cat. No. 04693116001, Roche
      Applied Science),
      Phosphatase inhibitors
      Reducing agent: 1 mM DTT
      Boiling for ≥5 min? no
      Protein loaded ug/lane or cells/lane: 60 ug
      Positive control: V5-tagged constructs were detected with anti-V5 antibody
      Negative control: empty cells
      5) Percentage of gel: 10%
      Type of membrane : nitrocellulose membrane
      Protein transfer verified: by ponceau stain
      Blocking agent and concentration: 5% Milk in TBS + 0.1% Tween 20
      Blocking time: 2 h
      Blocking temperature : RT
      6) Was a fresh membrane used or had it been probed and stripped with a
      different antibody? Yes
      7) Primary antibody (If more than one was used, describe in
      “additional notes”) : Mouse monoclonal anti-V5 (R960-25, Invitrogen)
      Concentration or dilution: 1:5,000
      Diluent buffer : 3% BSA in TBS
      Incubation time: 16 h
      Incubation temperature: 8 C
      8) Secondary antibody: goat anti-mouse IgGHRP (biorad# 170-6516)
      Species:
      Reacts against: mouse
      Concentration or dilution: 1:25,000
      Diluent buffer : 3% BSA in TBS
      Incubation time: 45 min
      Incubation temperature: RT
      Fluorochrome or enzyme conjugate:IgG-HRP
      9) Washing after primary and secondary antibodies:
      Buffer
      Number of washes: 2
      10)Detection method: autoradiography
      11) How many times have you run this staining? 3
      Do you obtain the same results every time? Yes
      What steps have you altered to try and optimize the use of this antibody?
      the anti-V5 antibody used has already been tested in the lab and found
      to work well.
      BR
      Enzo
      --
      Enzo Scifo, PhD candidate
      Meilahti Clinical Proteomics Core Facility
      Institute of Biomedicine/Anatomy
      P.O.Box 63 (Haartmaninkatu 8)
      FI-00014 University of Helsinki
      Helsinki, Finland
      Tel: +358919125202
      Fax: +358919125206
      Mobile: +358466370309

      Read More
      Answer

      Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

      The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I can confirm that unfortunately, as you purchased this product farover 180 days ago it is will no longer be covered by our Abpromise guarantee on this occasion. Details of our guarantee are on our website and we encourage customers to contact us as soon as possible if they experience any difficulties.If a particular antibody causes problems we do encourage customers to contact us as soon as possible to save time and material. Although not covered by our guarantee, we would still be pleased to offer some suggestions to help optimize the results

      Having reviewed this case, I would like to offer some suggestions to help optimize the resultsandI would also appreciate if you can confirm some further details:

      1. What's the species you were using to detect the proteins: human, mouse,etc. proteins with a tag?

      2. Almost all of our antibodies are developed to work under denatured conditions or otherwise it will be stated on the datasheet. It may be that the antibody cannot detect itsepitope as it may be simply not accessible. Please boil your samples to provide optimal conditions. The same goes for the reducing conditions of the gel which are usually necessary forthe most our antibodies to work properly.

      3.In order to avoid over-loading and therefore to decrease the side bands, I would like to suggest loading 20 to 30 ug protein per lane.

      3. What arethe listedprimaries antibodies concentration?Or do you use 1:5000 for all of them?

      4. What kind of secondary antibodies are you using for the listed primaries? Do you perform a non-primary control with them beforehand?

      5. Is it possible to send us an image?

      6. How did you store the antibodies?


      Should the suggestions not improve the results, please do let me know.


      I hope this information is helpful, and I thank you for your cooperation.

      Read More

      Question

      Product code: 15575
      Lot number: GR38115
      Inquiry: Dear Sir/Madam, I have previously made orders for primary antibodies (ref: #xxxxx)and #xxxx) and lately realised that they are non-specific. The tests were done by western blot on V5-tagged constructs expressing SEC61A, CPT1A, DBH and SYNGR3. Probing with the anti-V5 antibody identified the proteins of interest, which were undectable by the mentioned antibodies. 1 x SEC61A antibody ab15575 100.0UL EUR 360.00 1 x CPT1A antibody ab53532 100.0UL EUR 360.00 1 x Dopamine beta Hydroxylase antibody ab96615 100.0UL EUR 360.00 1 x Synaptogyrin 3 antibody ab106460 100.0UG EUR 360.00 Kindly inform me how to obtain replacements for the above mentioned antibodies. Best Regards

      Read More
      Answer

      Thank you for contacting us.

      I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.


      If you wouldn't mind, I would like to try to understand what may be contributing to the problems encountered in order to hopefully resolve the situation. To this end, could you please fill in the questionnaire I have attached to this email. If you could include images of the results obtained (including that using the V5 antibody) that would be very helpful.

      I also have a few additional questions:

      1. How were the V5-protein construct made? Which sequences were used to construct the fusion protein (human/mouse/rat etc)?

      2. Which V5 antibody was used? And which secondary antibody was used with this antibody?

      3. From your orders I can see that you also purchased ab80464, ab60332 and ab84589, were you using the same protocol using these (V5 tagging?)? Did you have any problems in using these antibodies as well?

      OnceIhavereceive this information and the completed questionnaire,I will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary.

      I look forward to receiving your reply.

      Read More

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