重组Anti-CPSF73抗体[EPR7468] (ab131245)
![Western blot - Anti-CPSF73 antibody [EPR7468] (ab131245)](https://www.abcam.cn/ps/products/131/ab131245/Images/ab131245-145567-anti-cpsf73-antibody-epr7468-western-blot.jpg "Western blot - Anti-CPSF73 antibody [EPR7468] (ab131245)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7468] to CPSF73
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-CPSF73抗体[EPR7468]
参阅全部 CPSF73 一抗 -
描述
兔单克隆抗体[EPR7468] to CPSF73 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IFmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human CPSF73 aa 650-750. The exact sequence is proprietary.
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阳性对照
- WB: HeLa, 293T, Jurkat, Caco-2, RAW 264.7, PC-12, and NIH/3T3 cell lysates. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 1.70 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR7468 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131245于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/250.
|
|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 77 kDa.
|
| ICC/IF | (1) |
1/50 - 1/100.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/250. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 77 kDa. |
|
ICC/IF
1/50 - 1/100. |
应用说明
Is unsuitable for IHC-P or IP.
靶标
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功能
Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. -
序列相似性
Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF3 subfamily. -
翻译后修饰
Sumoylated on Lys-462, Lys-465 and Lys-545, preferentially by SUMO3. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 51692 Human
- Entrez Gene: 54451 Mouse
- Entrez Gene: 298916 Rat
- Omim: 606029 Human
- SwissProt: Q9UKF6 Human
- SwissProt: Q9QXK7 Mouse
- Unigene: 515972 Human
- Unigene: 356778 Mouse
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别名
- Cleavage and polyadenylation specific factor 3 73kDa antibody
- Cleavage and polyadenylation specificity factor 73 kDa subunit antibody
- Cleavage and polyadenylation specificity factor subunit 3 antibody
see all
图片
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Western blot - Anti-CPSF73 antibody [EPR7468] (ab131245)All lanes : Anti-CPSF73 antibody [EPR7468] (ab131245) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Caco-2 cell lysate
Lane 5 : RAW 264.7 cell lysate
Lane 6 : PC-12 cell lysate
Lane 7 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 77 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-CPSF73 antibody [EPR7468] (ab131245)](https://www.abcam.cn/ps/products/131/ab131245/Images/ab131245-145566-anti-cpsf73-antibody-epr7468-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CPSF73 antibody [EPR7468] (ab131245)Immunofluorescence analysis of HeLa cells labelling CPSF73 with ab131245 at 1/50 dilution. -
![Flow Cytometry (Intracellular) - Anti-CPSF73 antibody [EPR7468] (ab131245)](https://www.abcam.cn/ps/products/131/ab131245/Images/ab131245-280419-anti-cpsf73-antibody-epr7468-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-CPSF73 antibody [EPR7468] (ab131245)Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling CPSF73 (red) with ab131245 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.
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![Western blot - Anti-CPSF73 antibody [EPR7468] (ab131245)](https://www.abcam.cn/ps/products/131/ab131245/Images/ab131245-145565-anti-cpsf73-antibody-epr7468-western-blot.jpg)
Western blot - Anti-CPSF73 antibody [EPR7468] (ab131245)Anti-CPSF73 antibody [EPR7468] (ab131245) at 1/1000 dilution + U2OS whole cell lysate at 100 µg
Predicted band size: 77 kDa
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![OI-RD Scanning - Anti-CPSF73 antibody [EPR7468] (ab131245)](https://www.abcam.cn/ps/products/131/ab131245/Images/ab131245-145564-anti-cpsf73-antibody-epr7468.jpg)
OI-RD Scanning - Anti-CPSF73 antibody [EPR7468] (ab131245) -
![Anti-CPSF73 antibody [EPR7468] (ab131245)](https://www.abcam.cn/ps/products/131/ab131245/Images/ab131245-6-benefits-of-recombinant-antibodies.png)
Anti-CPSF73 antibody [EPR7468] (ab131245)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab131245 被引用在 2 文献中.
- Yang L et al. SP1 induced long non-coding RNA LINC00958 overexpression facilitate cell proliferation, migration and invasion in lung adenocarcinoma via mediating miR-625-5p/CPSF7 axis. Cancer Cell Int 20:24 (2020). PubMed: 31997940
- Davidson L et al. Rapid Depletion of DIS3, EXOSC10, or XRN2 Reveals the Immediate Impact of Exoribonucleolysis on Nuclear RNA Metabolism and Transcriptional Control. Cell Rep 26:2779-2791.e5 (2019). PubMed: 30840897