重组Anti-CPSF30/NAR抗体[EPR7433] (ab131218)
![Western blot - Anti-CPSF30/NAR antibody [EPR7433] (ab131218)](https://www.abcam.cn/ps/products/131/ab131218/Images/CPSF30-Primary-antibodies-ab131218-1.jpg "Western blot - Anti-CPSF30/NAR antibody [EPR7433] (ab131218)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7433] to CPSF30/NAR
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-CPSF30/NAR抗体[EPR7433]
参阅全部 CPSF30/NAR 一抗 -
描述
兔单克隆抗体[EPR7433] to CPSF30/NAR -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
-
免疫原
Synthetic peptide within Human CPSF30/NAR aa 1-100. The exact sequence is proprietary.
Database link: O95639 -
阳性对照
- Jurkat, 293T, and HepG2 cell lysates.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 2.69 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7433 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131218于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 30 kDa. |
应用说明
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
靶标
-
功能
Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. CPSF4 binds RNA polymers with a preference for poly(U). -
序列相似性
Belongs to the CPSF4/YTH1 family.
Contains 5 C3H1-type zinc fingers.
Contains 1 CCHC-type zinc finger. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 10898 Human
- Entrez Gene: 54188 Mouse
- Entrez Gene: 304277 Rat
- Omim: 603052 Human
- SwissProt: O95639 Human
- SwissProt: Q8BQZ5 Mouse
- SwissProt: Q5FVR7 Rat
- Unigene: 489287 Human
see all -
别名
- cleavage and polyadenylation specific factor 4, 30kDa antibody
- Cleavage and polyadenylation specificity factor 30 kDa subunit antibody
- Cleavage and polyadenylation specificity factor subunit 4 antibody
see all
图片
-
Western blot - Anti-CPSF30/NAR antibody [EPR7433] (ab131218)All lanes : Anti-CPSF30/NAR antibody [EPR7433] (ab131218) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 30 kDa -
![OI-RD Scanning - Anti-CPSF30/NAR antibody [EPR7433] (ab131218)](https://www.abcam.cn/ps/products/131/ab131218/Images/CPSF30-Primary-antibodies-ab131218-2.jpg)
OI-RD Scanning - Anti-CPSF30/NAR antibody [EPR7433] (ab131218) -
![Anti-CPSF30/NAR antibody [EPR7433] (ab131218)](https://www.abcam.cn/ps/products/131/ab131218/Images/ab131218-3-benefits-of-recombinant-antibodies.png)
Anti-CPSF30/NAR antibody [EPR7433] (ab131218)
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab131218 被引用在 1 文献中.
- Bergant V et al. mRNA 3'UTR lengthening by alternative polyadenylation attenuates inflammatory responses and correlates with virulence of Influenza A virus. Nat Commun 14:4906 (2023). PubMed: 37582777