重组Anti-CPSF2/CPSF100抗体[EPR7599] (ab126760)
![Western blot - Anti-CPSF2/CPSF100 antibody [EPR7599] (ab126760)](https://www.abcam.cn/ps/products/126/ab126760/Images/ab126760-140788-anti-cpsf2-antibody-epr7599-western-blot.jpg "Western blot - Anti-CPSF2/CPSF100 antibody [EPR7599] (ab126760)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7599] to CPSF2/CPSF100
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CPSF2/CPSF100抗体[EPR7599]
参阅全部 CPSF2/CPSF100 一抗 -
描述
兔单克隆抗体[EPR7599] to CPSF2/CPSF100 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human CPSF2/CPSF100 aa 700-800. The exact sequence is proprietary.
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阳性对照
- HeLa, Ramos, Jurkat, and 293T cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7599 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126760于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (4) |
1/1000 - 1/10000. Detects a band of approximately 103 kDa (predicted molecular weight: 88 kDa).
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 103 kDa (predicted molecular weight: 88 kDa). |
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
靶标
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功能
Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Involved in the histone 3' end pre-mRNA processing. -
序列相似性
Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF2/YSH1 subfamily. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 53981 Human
- Entrez Gene: 51786 Mouse
- Entrez Gene: 299256 Rat
- Omim: 606028 Human
- SwissProt: Q9P2I0 Human
- SwissProt: O35218 Mouse
- SwissProt: D3Z9E6 Rat
- Unigene: 657632 Human
see all -
别名
- Cleavage and polyadenylation specific factor 2 antibody
- cleavage and polyadenylation specific factor 2, 100kDa antibody
- Cleavage and polyadenylation specificity factor 100 kDa subunit antibody
see all
图片
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Western blot - Anti-CPSF2/CPSF100 antibody [EPR7599] (ab126760)All lanes : Anti-CPSF2/CPSF100 antibody [EPR7599] (ab126760) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 88 kDa -
![Anti-CPSF2/CPSF100 antibody [EPR7599] (ab126760)](https://www.abcam.cn/ps/products/126/ab126760/Images/ab126760-2-benefits-of-recombinant-antibodies.png)
Anti-CPSF2/CPSF100 antibody [EPR7599] (ab126760)
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab126760 被引用在 2 文献中.
- Yu S et al. SWI/SNF interacts with cleavage and polyadenylation factors and facilitates pre-mRNA 3' end processing. Nucleic Acids Res 46:8557-8573 (2018). PubMed: 29860334
- Guillén-Boixet J et al. CPEB4 is regulated during cell cycle by ERK2/Cdk1-mediated phosphorylation and its assembly into liquid-like droplets. Elife 5:N/A (2016). PubMed: 27802129