重组Anti-CPS1抗体[EPR7494] (ab128942)
![Western blot - Anti-CPS1 antibody [EPR7494] (ab128942)](https://www.abcam.cn/ps/products/128/ab128942/Images/ab128942-142818-anti-cps1-antibody-epr7494-western-blot.jpg "Western blot - Anti-CPS1 antibody [EPR7494] (ab128942)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7494] to CPS1
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CPS1抗体[EPR7494]
参阅全部 CPS1 一抗 -
描述
兔单克隆抗体[EPR7494] to CPS1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, Flow Cyt (Intra)more details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human CPS1 aa 1450-1550. The exact sequence is proprietary.
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阳性对照
- HeLa cell lysate; Human fetal liver lysate; Human liver tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7494 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128942于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 165 kDa).
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 165 kDa). |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
靶标
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功能
Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell. -
组织特异性
Primarily in the liver and small intestine. -
疾病相关
Defects in CPS1 are the cause of carbamoyl phosphate synthetase 1 deficiency (CPS1D) [MIM:237300]. CPS1D is an autosomal recessive disorder of the urea cycle causing hyperammonemia. Clinical features include protein intolerance, intermittent ataxia, seizures, lethargy, developmental delay and mental retardation.
Note=Genetic variations in CPS1 influence the availability of precursors for nitric oxide (NO) synthesis and play a role in clinical situations where endogenous NO production is critically important, such as neonatal pulmonary hypertension, increased pulmonary artery pressure following surgical repair of congenital heart defects or hepatovenocclusive disease following bone marrow transplantation. Infants with neonatal pulmonary hypertension homozygous for Thr-1406 have lower L-arginine concentrations than neonates homozygous for Asn-1406. -
序列相似性
Contains 2 ATP-grasp domains.
Contains 1 glutamine amidotransferase type-1 domain. -
结构域
The type-1 glutamine amidotransferase domain is defective. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 1373 Human
- Omim: 608307 Human
- SwissProt: P31327 Human
- Unigene: 149252 Human
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别名
- Carbamoyl phosphate synthase [ammonia] antibody
- Carbamoyl phosphate synthase [ammonia] mitochondrial antibody
- Carbamoyl phosphate synthase antibody
see all
图片
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Western blot - Anti-CPS1 antibody [EPR7494] (ab128942)All lanes : Anti-CPS1 antibody [EPR7494] (ab128942) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Human fetal liver tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 165 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted? -
![Flow Cytometry (Intracellular) - Anti-CPS1 antibody [EPR7494] (ab128942)](https://www.abcam.cn/ps/products/128/ab128942/Images/ab128942-275856-anti-cps1-antibody-epr7494-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-CPS1 antibody [EPR7494] (ab128942)Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling CPS1 with unpurified ab128942 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti-rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPS1 antibody [EPR7494] (ab128942)](https://www.abcam.cn/ps/products/128/ab128942/Images/ab128942-142817-anti-cps1-antibody-epr7494-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPS1 antibody [EPR7494] (ab128942)ab128942, at 1/250 dilution, staining CPS1 in paraffin-embedded Human liver tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-CPS1 antibody [EPR7494] (ab128942)](https://www.abcam.cn/ps/products/128/ab128942/Images/ab128942-4-benefits-of-recombinant-antibodies.png)
Anti-CPS1 antibody [EPR7494] (ab128942)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab128942 被引用在 2 文献中.
- Fang X et al. Expression profiling of CPS1 in Correa's cascade and its association with gastric cancer prognosis. Oncol Lett 21:441 (2021). PubMed: 33868479
- Hu CL et al. 3D Culture of Circulating Tumor Cells for Evaluating Early Recurrence and Metastasis in Patients with Hepatocellular Carcinoma. Onco Targets Ther 14:2673-2688 (2021). PubMed: 33888992