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Anti-CPS1抗体(ab3682)

  • Datasheet
  • SDS
Reviews (9)Q&A (3)References (21)

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Western blot - Anti-CPS1 antibody (ab3682)
  • Immunocytochemistry/ Immunofluorescence - Anti-CPS1 antibody (ab3682)
  • Western blot - Anti-CPS1 antibody (ab3682)

Key features and details

  • Rabbit polyclonal to CPS1
  • Suitable for: IHC-Fr, IHC-P, IP, ICC/IF, WB
  • Reacts with: Mouse, Rat, African green monkey
  • Isotype: IgG

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概述

  • 产品名称

    Anti-CPS1抗体
    参阅全部 CPS1 一抗
  • 描述

    兔多克隆抗体to CPS1
  • 宿主

    Rabbit
  • 特异性

    When the antibody is used at 1/2000 some lower molecular weight bands appear in COS-7 cells, the extent of which is significantly reduced when the antibody is used at 1/10,000.
  • 经测试应用

    适用于: IHC-Fr, IHC-P, IP, ICC/IF, WBmore details
  • 种属反应性

    与反应: Mouse, Rat, African green monkey
  • 免疫原

    Full length native protein (purified) corresponding to Rat CPS1.

  • 常规说明

    OMIM reference: 114010 Alternative name: carbamoylphosphate synthetase 1, CAD

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • 纯度

    Whole antiserum
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) (ab99697)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human CPS1 protein (ab152296)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3682于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-Fr (2)
Use at an assay dependent concentration.
IHC-P (1)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ICC/IF (1)
Use at an assay dependent concentration.
WB (5)
Use at an assay dependent concentration.
说明
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

靶标

  • 功能

    Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell.
  • 组织特异性

    Primarily in the liver and small intestine.
  • 疾病相关

    Defects in CPS1 are the cause of carbamoyl phosphate synthetase 1 deficiency (CPS1D) [MIM:237300]. CPS1D is an autosomal recessive disorder of the urea cycle causing hyperammonemia. Clinical features include protein intolerance, intermittent ataxia, seizures, lethargy, developmental delay and mental retardation.
    Note=Genetic variations in CPS1 influence the availability of precursors for nitric oxide (NO) synthesis and play a role in clinical situations where endogenous NO production is critically important, such as neonatal pulmonary hypertension, increased pulmonary artery pressure following surgical repair of congenital heart defects or hepatovenocclusive disease following bone marrow transplantation. Infants with neonatal pulmonary hypertension homozygous for Thr-1406 have lower L-arginine concentrations than neonates homozygous for Asn-1406.
  • 序列相似性

    Contains 2 ATP-grasp domains.
    Contains 1 glutamine amidotransferase type-1 domain.
  • 结构域

    The type-1 glutamine amidotransferase domain is defective.
  • 细胞定位

    Mitochondrion.
  • Target information above from: UniProt accession P31327 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 227231 Mouse
    • Entrez Gene: 497840 Rat
    • SwissProt: Q8C196 Mouse
    • SwissProt: P07756 Rat
    • Unigene: 343942 Mouse
    • Unigene: 53968 Rat
    • 别名

      • Carbamoyl phosphate synthase [ammonia] antibody
      • Carbamoyl phosphate synthase [ammonia] mitochondrial antibody
      • Carbamoyl phosphate synthase antibody
      • Carbamoyl phosphate synthetase 1 antibody
      • Carbamoyl phosphate synthetase 1 mitochondrial antibody
      • Carbamoyl phosphate synthetase I antibody
      • Carbamoyl-phosphate synthase [ammonia] antibody
      • Carbamoyl-phosphate synthetase I antibody
      • Carbamoylphosphate synthase antibody
      • Carbamoylphosphate synthetase 1 antibody
      • Carbamoylphosphate synthetase I antibody
      • CPS 1 antibody
      • Cps1 antibody
      • CPSase 1 antibody
      • CPSase I antibody
      • CPSASE1 antibody
      • CPSM_HUMAN antibody
      • mitochondrial antibody
      • MS738 antibody
      see all

    图片

    • Western blot - Anti-CPS1 antibody (ab3682)
      Western blot - Anti-CPS1 antibody (ab3682)

      Lane 1: 5 ul of 2ml lysate cos-7 cells transfected with full length CPS 1. Before IP
      Lane 2: cos-7 cells transfected with full length CPS 1. IP with 5 ul of pre-immune serum
      Lane 3: cos-7 cells transfected with full length CPS 1. IP with 2 ul of polyclonal antibody anti CPS 1 (ab3682)
      Lane 4: cos-7 cells transfected with full length CPS 1. IP with 5 ul of polyclonal antibody anti CPS 1 (ab3682)
      Lane 5: 5 ul of 2ml lysate cos-7 cells transfected with empty vector (pCMV5-tr). Before IP
      Lane 6: cos-7 cells transfected with empty vector (pCMV5-tr). IP with 5 ul of polyclonal antibody anti CPS 1 (ab3682)
      Lane 7: 5 ul of 2ml lysate untransfected cos-7 cells. Before IP
      Lane 8: untransfected cos-7 cells. IP with 5 ul of polyclonal antibody anti CPS 1 (ab3682) Ab3682 can IP CPS1 transiently expressed in COS-7 cells.

      Lane 1: 5 ul of 2ml lysate cos-7 cells transfected with full length CPS 1. Before IP
      Lane 2: cos-7 cells transfected with full length CPS 1. IP

    • Immunocytochemistry/ Immunofluorescence - Anti-CPS1 antibody (ab3682)
      Immunocytochemistry/ Immunofluorescence - Anti-CPS1 antibody (ab3682)

      CPS-1 localisation is consistent with the reticulated pattern of liver mitonchondria.

      Rabbit polyclonal to CPS-1 (ab3682), at 1/5000 on COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV5-CPS 1 plasmid (Corvi, M.M and Berthiaume, L.G. unpublished data).

      Secondary: donkey anti-rabbit (Texas Red).

    • Western blot - Anti-CPS1 antibody (ab3682)
      Western blot - Anti-CPS1 antibody (ab3682)

      Western blot using Rabbit polyclonal to CPS 1 (ab3682). All lanes loaded with 100 ng of purified CPS 1
      Lane 1: ab3682 1/500
      Lane 2: ab3682 1/1000
      Lane 3: ab3682 1/2000 Western blot using Rabbit polyclonal to CPS 1 (ab3682).

      All lanes loaded with 100 ng of purified CPS 1

      Lane 1: ab3682 1/500
      Lane 2: ab3682 1/1000
      Lane 3: ab3682 1/2000

    实验方案

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (21)

    发表研究结果有使用 ab3682?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3682 被引用在 21 文献中.

    • Kim J  et al. The hexosamine biosynthesis pathway is a targetable liability in KRAS/LKB1 mutant lung cancer. Nat Metab 2:1401-1412 (2020). PubMed: 33257855
    • Amano H  et al. Telomere Dysfunction Induces Sirtuin Repression that Drives Telomere-Dependent Disease. Cell Metab 29:1274-1290.e9 (2019). PubMed: 30930169
    • Alsaeedi F  et al. Expression of serine/threonine protein kinase SGK1F promotes an hepatoblast state in stem cells directed to differentiate into hepatocytes. PLoS One 14:e0218135 (2019). PubMed: 31242206
    • Tata PR  et al. Developmental History Provides a Roadmap for the Emergence of Tumor Plasticity. Dev Cell 44:679-693.e5 (2018). PubMed: 29587142
    • Wang T  et al. L-leucine stimulates glutamate dehydrogenase activity and glutamate synthesis by regulating mTORC1/SIRT4 pathway in pig liver. Anim Nutr 4:329-337 (2018). PubMed: 30175263
    View all Publications for this product

    客户评价及客户问答

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    1-10 of 12 Abreviews or Q&A

    Western blot abreview for Anti-CPS1 antibody - Liver Mitochondrial Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (12%)
    Sample
    Rat Tissue lysate - whole (Liver)
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Nov 11 2013

    Western blot abreview for Anti-CPS1 antibody - Liver Mitochondrial Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (12%)
    Sample
    Mouse Tissue lysate - whole (Liver)
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Nov 07 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CPS1 antibody - Liver Mitochondrial Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Hepatocyte)
    Specification
    Hepatocyte
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2%
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 12 2013

    Western blot abreview for Anti-CPS1 antibody - Liver Mitochondrial Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Liver)
    Loading amount
    50 µg
    Specification
    Liver
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 25 2012

    Western blot abreview for Anti-CPS1 antibody - Liver Mitochondrial Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Liver)
    Loading amount
    50 µg
    Specification
    Liver
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 25 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-CPS1 antibody - Liver Mitochondrial Marker

    Good
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Liver)
    Specification
    Liver
    Fixative
    Acetone
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 06 2012

    Western blot abreview for Anti-CPS1 antibody - Liver Mitochondrial Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (liver)
    Loading amount
    40 µg
    Specification
    liver
    Treatment
    17.5 mg/kg retrorsine for 24 h
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Junyi Xue

    Verified customer

    提交于 Jul 03 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CPS1 antibody - Liver Mitochondrial Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (liver E18.5)
    Specification
    liver E18.5
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Permeabilization
    No
    Blocking step
    (agent) for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Asha Seth

    Verified customer

    提交于 Jun 23 2009

    Immunohistochemistry (Frozen sections) abreview for Anti-CPS1 antibody - Liver Mitochondrial Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Liver PO)
    Specification
    Liver PO
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    maleate blocking solution as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Asha Seth

    Verified customer

    提交于 Jun 23 2009

    Question

    Rezepte für Melate-Puffer

    Read More

    Abcam community

    Verified customer

    Asked on Aug 13 2012

    Answer

    Vielen Dank für Ihren Anruf heute Morgen.
    Ich habe mir die Beschreibung des IHC-P Bildes nochmals angeschaut und muss ihnen leider mitteilen, dass ich Ihnen keine genauen Angaben über dem Maleate Puffer machen kann, da dieses Bild und die Beschreibung nicht aus unsrem Labor stammt. Eine Kundin hat einen Abreview als Feedback mi t diesem Bild eingeschickt.
    https://www.abcam.com/CPS1-antibody-ab3682.html&tab=abreviews&intabreviewid=14931
    Ich habe allerding Rezepte für Melate-Puffer gefunden:
    http://www.nrims.harvard.edu/protocols/Uranyl_Acetate_Stain_in_Maleate_Buffer.pdf
    http://www.ou.edu/research/electron/bmz5364/maleate.html
    Ich hoffe, diese helfen Ihnen weiter und wünsche Ihnen viel Erfolg mit Ihren Experimenten.

    Read More

    Abcam Scientific Support

    回复于 Aug 13 2012

    1-10 of 12 Abreviews or Q&A

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