Anti-CPS1抗体[2E4AE11] (ab110303)
![Flow Cytometry - Anti-CPS1 antibody [2E4AE11] (ab110303)](https://www.abcam.cn/ps/products/110/ab110303/Images/ab110303-123558-anti-cps1-antibody-2e4ae11-flow-cytometry.gif "Flow Cytometry - Anti-CPS1 antibody [2E4AE11] (ab110303)")
Key features and details
- Mouse monoclonal [2E4AE11] to CPS1
- Suitable for: IHC-P, Flow Cyt, ICC, IP
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-CPS1抗体[2E4AE11]
参阅全部 CPS1 一抗 -
描述
小鼠单克隆抗体[2E4AE11] to CPS1 -
宿主
Mouse -
经测试应用
适用于: IHC-P, Flow Cyt, ICC, IPmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HDFn and HeLa cells; Human liver mitochondria lysate.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
ab110303 was produced in vitro using hybridomas grown in serum-free medium. Purity: >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
2E4AE11 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110303于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
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| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| ICC |
Use a concentration of 10 µg/ml.
|
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| IP |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
ICC
Use a concentration of 10 µg/ml. |
|
IP
Use at an assay dependent concentration. |
靶标
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功能
Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell. -
组织特异性
Primarily in the liver and small intestine. -
疾病相关
Defects in CPS1 are the cause of carbamoyl phosphate synthetase 1 deficiency (CPS1D) [MIM:237300]. CPS1D is an autosomal recessive disorder of the urea cycle causing hyperammonemia. Clinical features include protein intolerance, intermittent ataxia, seizures, lethargy, developmental delay and mental retardation.
Note=Genetic variations in CPS1 influence the availability of precursors for nitric oxide (NO) synthesis and play a role in clinical situations where endogenous NO production is critically important, such as neonatal pulmonary hypertension, increased pulmonary artery pressure following surgical repair of congenital heart defects or hepatovenocclusive disease following bone marrow transplantation. Infants with neonatal pulmonary hypertension homozygous for Thr-1406 have lower L-arginine concentrations than neonates homozygous for Asn-1406. -
序列相似性
Contains 2 ATP-grasp domains.
Contains 1 glutamine amidotransferase type-1 domain. -
结构域
The type-1 glutamine amidotransferase domain is defective. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 1373 Human
- Omim: 608307 Human
- SwissProt: P31327 Human
- Unigene: 149252 Human
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别名
- Carbamoyl phosphate synthase [ammonia] antibody
- Carbamoyl phosphate synthase [ammonia] mitochondrial antibody
- Carbamoyl phosphate synthase antibody
see all
图片
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Flow Cytometry - Anti-CPS1 antibody [2E4AE11] (ab110303)Flow cytometric analysis using ab110303 at 1µg/ml staining CPS1 in HeLa cells (blue). Isotype control antibody (red).
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![Immunocytochemistry - Anti-CPS1 antibody [2E4AE11] (ab110303)](https://www.abcam.cn/ps/products/110/ab110303/Images/ab110303-123560-anti-cps1-antibody-2e4ae11-immunocytochemistry.jpg)
Immunocytochemistry - Anti-CPS1 antibody [2E4AE11] (ab110303)Immunocytochemistry analysis using ab110303 at 10µg/ml staining CPS1 in HDFn cells (paraformaldehyde fixed and Triton X-100 permeabilized). The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria. -
![Immunoprecipitation - Anti-CPS1 antibody [2E4AE11] (ab110303)](https://www.abcam.cn/ps/products/110/ab110303/Images/ab110303-123559-anti-cps1-antibody-2e4ae11-immunoprecipitation.gif)
Immunoprecipitation - Anti-CPS1 antibody [2E4AE11] (ab110303)Detection of ab110303 by immunoprecipitation staining of 164kDa CPS1, a liver mitochondrial marker in human liver mitochondrial lysate. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPS1 antibody [2E4AE11] (ab110303)](https://www.abcam.cn/ps/products/110/ab110303/Images/ab110303-123557-anti-cps1-antibody-2e4ae11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPS1 antibody [2E4AE11] (ab110303)IHC image of ab110303 staining in human duodenum formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110303, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab110303 被引用在 2 文献中.
- Sun B et al. miR-2392 functions as tumour suppressor and inhibits malignant progression of hepatocellular carcinoma via directly targeting JAG2. Liver Int 42:1658-1673 (2022). PubMed: 35485355
- Li J et al. Detection of circulating tumor cells in hepatocellular carcinoma using antibodies against asialoglycoprotein receptor, carbamoyl phosphate synthetase 1 and pan-cytokeratin. PLoS One 9:e96185 (2014). Flow Cyt ; Human . PubMed: 24763545