AB150405

重组Anti-CPM抗体[EPR8052]

Anti-CPM antibody [EPR8052]

RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal CPM antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications.

查看别名

Carboxypeptidase M, CPM

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPM antibody [EPR8052] (AB150405)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPM antibody [EPR8052] (AB150405)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling CPM with unpurified ab150405 at a dilution of 1/50.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPM antibody [EPR8052] (AB150405)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human uterus tissue labelling CPM with unpurified ab150405 at a dilution of 1/50.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPM antibody [EPR8052] (AB150405)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue labelling CPM with purified ab150405 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051 a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Unknown

Western blot - Anti-CPM antibody [EPR8052] (AB150405)

All lanes:

Western blot - Anti-CPM antibody [EPR8052] (ab150405) at 1/1000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Lane 3:

Human fetal liver tissue lysate at 10 µg

Lane 4:

Human placenta lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 51 kDa

Observed band size: 62 kDa

false

Lab

Western blot - Anti-CPM antibody [EPR8052] (AB150405)

Blocking and dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-CPM antibody [EPR8052] (ab150405) at 1/5000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 51 kDa

Observed band size: 62 kDa

false

Lab

Western blot - Anti-CPM antibody [EPR8052] (AB150405)

Blocking and dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-CPM antibody [EPR8052] (ab150405) at 1/2500 dilution

All lanes:

Human placenta tissue lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 51 kDa

Observed band size: 62 kDa

false

不同偶联物与剂型 (8)

Carrier free

Anti-CPM antibody [EPR8052] - BSA and Azide free
660 APC

APC Anti-CPM antibody [EPR8052]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CPM antibody [EPR8052]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-CPM antibody [EPR8052]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-CPM antibody [EPR8052]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CPM antibody [EPR8052]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-CPM antibody [EPR8052]
578 PE

PE Anti-CPM antibody [EPR8052]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR8052

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/500", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Carboxypeptidase M (CPM) is an enzyme that cleaves C-terminal basic residues from peptides and proteins. Also known as carboxypeptidase M1 CPM has a molecular weight of approximately 50 kDa. It is expressed on the surface of various cell types such as macrophages epithelial cells and specific tumor cells. CPM functions optimally at physiological pH and high levels of expression are seen in the lung placenta and kidney tissues. This broad expression pattern suggests that CPM plays significant roles in diverse physiological contexts.

Biological function summary

CPM is involved in peptide processing and degradation contributing to the regulation of hormonal and neurotransmitter peptides. As part of a larger proteolytic system CPM modifies the activity and half-life of bioactive peptides by removing terminal residues. Its activity influences the regulation of peptide signaling pathways. In such roles it resounds in processes like local inflammatory response and modulation of cell surface receptors. CPM’s enzymatic activity ensures the proper function of the physiological regulatory mechanisms where peptidase activity is required.

Pathways

CPM is instrumental in the kallikrein-kinin and complement pathways. In the kallikrein-kinin system CPM regulates kinin levels influencing blood pressure and inflammation. It also relates to the complement system interacting with components that participate in immune responses. CPM’s function closely links to other carboxypeptidases in these pathways enhancing its role in regulating peptide effects or degradation. Its enzymatic interactions can influence the outcomes of these pathways ensuring an adequate biological response.

CPM has associations with conditions like hypertension and chronic inflammation. Its influence on the kallikrein-kinin system corresponds to implications for blood pressure regulation connecting CPM to pathways affecting hypertension. In chronic inflammatory conditions CPM affects the local inflammatory response by modulating active peptide levels. Related proteins like carboxypeptidase N and other members of the metallocarboxypeptidase family augment this functional network highlighting CPM’s relevance in managing peptide-mediated pathological processes.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Specifically removes C-terminal basic residues (Arg or Lys) from peptides and proteins. It is believed to play important roles in the control of peptide hormone and growth factor activity at the cell surface, and in the membrane-localized degradation of extracellular proteins.

See full target information Carboxypeptidase M

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica 147:65 PubMed38557897

2024

Landscape of brain myeloid cell transcriptome along the spatiotemporal progression of Alzheimer's disease reveals distinct sequential responses to Aβ and tau.

Applications

Unspecified application

Species

Unspecified reactive species

Astrid Wachter,Maya E Woodbury,Sylvia Lombardo,Aicha Abdourahman,Carolin Wuest,Emily McGlame,Timothy Pastika,Joseph Tamm,Nandini Romanul,Kiran Yanamandra,Rachel Bennett,Gen Lin,Taekyung Kwon,Fan Liao,Corinna Klein,Yelena Grinberg,Methasit Jaisa-Aad,Huan Li,Matthew P Frosch,Markus P Kummer,Sudeshna Das,Tammy Dellovade,Eric H Karran,Xavier Langlois,Janina S Ried,Alberto Serrano-Pozo,Robert V Talanian,Knut Biber,Bradley T Hyman

Antioxidants & redox signaling 35:917-950 PubMed34269613

2021

Oxidation of Hemoglobin Drives a Proatherogenic Polarization of Macrophages in Human Atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

László Potor,Zoltán Hendrik,Andreas Patsalos,Éva Katona,Gábor Méhes,Szilárd Póliska,Éva Csősz,Gergő Kalló,István Komáromi,Zsolt Combi,Niké Posta,Katalin Éva Sikura,Dávid Pethő,Melinda Oros,György Vereb,Csaba Tóth,Péter Gergely,László Nagy,György Balla,József Balla

Oncotarget 8:22674-22684 PubMed28186967

2017

MicroRNA-146a promote cell migration and invasion in human colorectal cancer via carboxypeptidase M/src-FAK pathway.

Applications

IHC-P, WB

Species

Human, Human

Di Lu,Qunyan Yao,Cheng Zhan,Zhang Le-Meng,Hongchun Liu,Yu Cai,Chuantao Tu,Xi Li,Yanting Zou,Shuncai Zhang

View all publications

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重组Anti-CPM抗体[EPR8052]